Amino acid dipepetide frequency for Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.145AlaAla: 6.145 ± 0.088	0.679AlaCys: 0.679 ± 0.024	3.525AlaAsp: 3.525 ± 0.052	4.987AlaGlu: 4.987 ± 0.075	3.632AlaPhe: 3.632 ± 0.066	5.269AlaGly: 5.269 ± 0.071	1.543AlaHis: 1.543 ± 0.042	6.177AlaIle: 6.177 ± 0.081	4.572AlaLys: 4.572 ± 0.075	7.95AlaLeu: 7.95 ± 0.09	2.164AlaMet: 2.164 ± 0.044	2.83AlaAsn: 2.83 ± 0.054	2.299AlaPro: 2.299 ± 0.05	2.416AlaGln: 2.416 ± 0.042	3.037AlaArg: 3.037 ± 0.061	4.103AlaSer: 4.103 ± 0.057	4.099AlaThr: 4.099 ± 0.068	5.561AlaVal: 5.561 ± 0.075	0.728AlaTrp: 0.728 ± 0.028	2.441AlaTyr: 2.441 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.451CysAla: 0.451 ± 0.018	0.099CysCys: 0.099 ± 0.009	0.374CysAsp: 0.374 ± 0.019	0.47CysGlu: 0.47 ± 0.021	0.382CysPhe: 0.382 ± 0.018	0.671CysGly: 0.671 ± 0.027	0.232CysHis: 0.232 ± 0.014	0.433CysIle: 0.433 ± 0.02	0.337CysLys: 0.337 ± 0.018	0.748CysLeu: 0.748 ± 0.025	0.175CysMet: 0.175 ± 0.012	0.25CysAsn: 0.25 ± 0.014	0.368CysPro: 0.368 ± 0.016	0.293CysGln: 0.293 ± 0.018	0.364CysArg: 0.364 ± 0.019	0.475CysSer: 0.475 ± 0.023	0.382CysThr: 0.382 ± 0.018	0.421CysVal: 0.421 ± 0.02	0.08CysTrp: 0.08 ± 0.007	0.251CysTyr: 0.251 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.277AspAla: 3.277 ± 0.057	0.338AspCys: 0.338 ± 0.018	2.835AspAsp: 2.835 ± 0.061	4.82AspGlu: 4.82 ± 0.079	2.103AspPhe: 2.103 ± 0.037	3.73AspGly: 3.73 ± 0.068	1.507AspHis: 1.507 ± 0.033	3.273AspIle: 3.273 ± 0.05	2.176AspLys: 2.176 ± 0.049	5.0AspLeu: 5.0 ± 0.068	1.302AspMet: 1.302 ± 0.042	1.303AspAsn: 1.303 ± 0.034	2.337AspPro: 2.337 ± 0.049	2.348AspGln: 2.348 ± 0.042	2.811AspArg: 2.811 ± 0.044	2.289AspSer: 2.289 ± 0.043	2.396AspThr: 2.396 ± 0.048	4.372AspVal: 4.372 ± 0.06	0.7AspTrp: 0.7 ± 0.03	1.97AspTyr: 1.97 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.502GluAla: 6.502 ± 0.087	0.418GluCys: 0.418 ± 0.017	3.761GluAsp: 3.761 ± 0.063	7.659GluGlu: 7.659 ± 0.125	2.215GluPhe: 2.215 ± 0.048	5.003GluGly: 5.003 ± 0.079	1.83GluHis: 1.83 ± 0.037	4.714GluIle: 4.714 ± 0.062	5.816GluLys: 5.816 ± 0.086	7.431GluLeu: 7.431 ± 0.078	2.304GluMet: 2.304 ± 0.049	2.933GluAsn: 2.933 ± 0.056	2.437GluPro: 2.437 ± 0.056	4.392GluGln: 4.392 ± 0.078	4.893GluArg: 4.893 ± 0.072	3.402GluSer: 3.402 ± 0.053	4.292GluThr: 4.292 ± 0.061	5.407GluVal: 5.407 ± 0.069	1.068GluTrp: 1.068 ± 0.034	1.962GluTyr: 1.962 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.371PheAla: 3.371 ± 0.056	0.359PheCys: 0.359 ± 0.016	2.259PheAsp: 2.259 ± 0.043	2.78PheGlu: 2.78 ± 0.045	2.266PhePhe: 2.266 ± 0.052	3.178PheGly: 3.178 ± 0.063	1.225PheHis: 1.225 ± 0.032	3.4PheIle: 3.4 ± 0.067	1.838PheLys: 1.838 ± 0.039	4.577PheLeu: 4.577 ± 0.077	1.119PheMet: 1.119 ± 0.032	1.525PheAsn: 1.525 ± 0.041	1.67PhePro: 1.67 ± 0.039	1.851PheGln: 1.851 ± 0.047	1.894PheArg: 1.894 ± 0.038	3.083PheSer: 3.083 ± 0.048	2.556PheThr: 2.556 ± 0.047	3.383PheVal: 3.383 ± 0.061	0.483PheTrp: 0.483 ± 0.023	1.69PheTyr: 1.69 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	5.361GlyAla: 5.361 ± 0.085	0.606GlyCys: 0.606 ± 0.028	3.448GlyAsp: 3.448 ± 0.065	5.046GlyGlu: 5.046 ± 0.073	3.482GlyPhe: 3.482 ± 0.063	4.998GlyGly: 4.998 ± 0.076	1.634GlyHis: 1.634 ± 0.044	5.224GlyIle: 5.224 ± 0.071	4.398GlyLys: 4.398 ± 0.061	6.923GlyLeu: 6.923 ± 0.093	2.163GlyMet: 2.163 ± 0.037	2.356GlyAsn: 2.356 ± 0.053	1.97GlyPro: 1.97 ± 0.038	2.501GlyGln: 2.501 ± 0.045	3.059GlyArg: 3.059 ± 0.054	3.852GlySer: 3.852 ± 0.062	4.249GlyThr: 4.249 ± 0.063	5.542GlyVal: 5.542 ± 0.077	0.839GlyTrp: 0.839 ± 0.028	2.778GlyTyr: 2.778 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.52HisAla: 1.52 ± 0.044	0.227HisCys: 0.227 ± 0.014	1.238HisAsp: 1.238 ± 0.037	1.767HisGlu: 1.767 ± 0.038	1.236HisPhe: 1.236 ± 0.03	1.732HisGly: 1.732 ± 0.035	0.853HisHis: 0.853 ± 0.027	1.431HisIle: 1.431 ± 0.036	0.888HisLys: 0.888 ± 0.031	2.611HisLeu: 2.611 ± 0.048	0.551HisMet: 0.551 ± 0.025	0.687HisAsn: 0.687 ± 0.022	1.3HisPro: 1.3 ± 0.034	1.028HisGln: 1.028 ± 0.029	1.216HisArg: 1.216 ± 0.031	1.366HisSer: 1.366 ± 0.037	1.18HisThr: 1.18 ± 0.029	2.027HisVal: 2.027 ± 0.046	0.284HisTrp: 0.284 ± 0.014	0.988HisTyr: 0.988 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.684IleAla: 5.684 ± 0.084	0.569IleCys: 0.569 ± 0.025	4.056IleAsp: 4.056 ± 0.057	5.389IleGlu: 5.389 ± 0.088	2.672IlePhe: 2.672 ± 0.063	5.826IleGly: 5.826 ± 0.076	1.757IleHis: 1.757 ± 0.038	4.622IleIle: 4.622 ± 0.08	3.326IleLys: 3.326 ± 0.063	6.105IleLeu: 6.105 ± 0.082	1.537IleMet: 1.537 ± 0.039	2.322IleAsn: 2.322 ± 0.045	3.276IlePro: 3.276 ± 0.049	2.891IleGln: 2.891 ± 0.043	3.384IleArg: 3.384 ± 0.054	4.078IleSer: 4.078 ± 0.063	3.655IleThr: 3.655 ± 0.059	5.549IleVal: 5.549 ± 0.071	0.626IleTrp: 0.626 ± 0.027	2.097IleTyr: 2.097 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.328LysAla: 4.328 ± 0.064	0.276LysCys: 0.276 ± 0.017	2.855LysAsp: 2.855 ± 0.057	6.033LysGlu: 6.033 ± 0.087	1.228LysPhe: 1.228 ± 0.035	4.231LysGly: 4.231 ± 0.074	1.364LysHis: 1.364 ± 0.035	3.161LysIle: 3.161 ± 0.055	4.699LysLys: 4.699 ± 0.079	4.875LysLeu: 4.875 ± 0.076	1.761LysMet: 1.761 ± 0.039	2.193LysAsn: 2.193 ± 0.044	2.079LysPro: 2.079 ± 0.043	3.183LysGln: 3.183 ± 0.059	3.867LysArg: 3.867 ± 0.067	2.549LysSer: 2.549 ± 0.047	3.046LysThr: 3.046 ± 0.054	4.159LysVal: 4.159 ± 0.067	0.705LysTrp: 0.705 ± 0.028	1.459LysTyr: 1.459 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	8.012LeuAla: 8.012 ± 0.087	0.713LeuCys: 0.713 ± 0.027	5.077LeuAsp: 5.077 ± 0.068	7.114LeuGlu: 7.114 ± 0.081	5.06LeuPhe: 5.06 ± 0.097	6.513LeuGly: 6.513 ± 0.102	2.389LeuHis: 2.389 ± 0.047	6.774LeuIle: 6.774 ± 0.098	5.707LeuLys: 5.707 ± 0.074	10.905LeuLeu: 10.905 ± 0.129	2.557LeuMet: 2.557 ± 0.051	3.579LeuAsn: 3.579 ± 0.056	4.228LeuPro: 4.228 ± 0.067	3.983LeuGln: 3.983 ± 0.067	4.237LeuArg: 4.237 ± 0.059	6.54LeuSer: 6.54 ± 0.085	5.991LeuThr: 5.991 ± 0.072	6.674LeuVal: 6.674 ± 0.084	0.913LeuTrp: 0.913 ± 0.029	3.201LeuTyr: 3.201 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.16MetAla: 2.16 ± 0.042	0.138MetCys: 0.138 ± 0.012	1.447MetAsp: 1.447 ± 0.034	2.104MetGlu: 2.104 ± 0.048	1.151MetPhe: 1.151 ± 0.032	1.689MetGly: 1.689 ± 0.04	0.422MetHis: 0.422 ± 0.018	2.165MetIle: 2.165 ± 0.052	2.326MetLys: 2.326 ± 0.044	2.492MetLeu: 2.492 ± 0.043	0.91MetMet: 0.91 ± 0.028	1.517MetAsn: 1.517 ± 0.034	0.956MetPro: 0.956 ± 0.034	0.816MetGln: 0.816 ± 0.026	1.186MetArg: 1.186 ± 0.032	1.659MetSer: 1.659 ± 0.036	1.859MetThr: 1.859 ± 0.041	1.87MetVal: 1.87 ± 0.041	0.227MetTrp: 0.227 ± 0.014	0.664MetTyr: 0.664 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.272AsnAla: 2.272 ± 0.047	0.211AsnCys: 0.211 ± 0.012	2.029AsnAsp: 2.029 ± 0.048	3.252AsnGlu: 3.252 ± 0.059	1.174AsnPhe: 1.174 ± 0.031	3.11AsnGly: 3.11 ± 0.057	1.027AsnHis: 1.027 ± 0.028	2.439AsnIle: 2.439 ± 0.048	1.788AsnLys: 1.788 ± 0.044	3.032AsnLeu: 3.032 ± 0.053	1.025AsnMet: 1.025 ± 0.027	1.346AsnAsn: 1.346 ± 0.043	1.855AsnPro: 1.855 ± 0.039	1.737AsnGln: 1.737 ± 0.037	1.973AsnArg: 1.973 ± 0.04	1.606AsnSer: 1.606 ± 0.039	1.626AsnThr: 1.626 ± 0.039	3.052AsnVal: 3.052 ± 0.051	0.462AsnTrp: 0.462 ± 0.021	1.18AsnTyr: 1.18 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	2.492ProAla: 2.492 ± 0.046	0.208ProCys: 0.208 ± 0.014	2.11ProAsp: 2.11 ± 0.044	3.133ProGlu: 3.133 ± 0.051	2.266ProPhe: 2.266 ± 0.046	2.367ProGly: 2.367 ± 0.053	0.969ProHis: 0.969 ± 0.028	2.8ProIle: 2.8 ± 0.051	1.909ProLys: 1.909 ± 0.042	4.009ProLeu: 4.009 ± 0.068	0.948ProMet: 0.948 ± 0.032	1.602ProAsn: 1.602 ± 0.039	1.242ProPro: 1.242 ± 0.034	1.147ProGln: 1.147 ± 0.036	1.343ProArg: 1.343 ± 0.032	2.549ProSer: 2.549 ± 0.063	2.251ProThr: 2.251 ± 0.051	3.029ProVal: 3.029 ± 0.052	0.457ProTrp: 0.457 ± 0.02	1.496ProTyr: 1.496 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.385GlnAla: 3.385 ± 0.059	0.215GlnCys: 0.215 ± 0.012	1.634GlnAsp: 1.634 ± 0.039	3.277GlnGlu: 3.277 ± 0.062	1.637GlnPhe: 1.637 ± 0.039	2.557GlnGly: 2.557 ± 0.046	0.938GlnHis: 0.938 ± 0.032	2.367GlnIle: 2.367 ± 0.046	2.569GlnLys: 2.569 ± 0.05	4.861GlnLeu: 4.861 ± 0.08	1.342GlnMet: 1.342 ± 0.037	1.215GlnAsn: 1.215 ± 0.031	1.466GlnPro: 1.466 ± 0.042	2.349GlnGln: 2.349 ± 0.066	1.928GlnArg: 1.928 ± 0.043	2.261GlnSer: 2.261 ± 0.045	2.403GlnThr: 2.403 ± 0.048	2.836GlnVal: 2.836 ± 0.049	0.56GlnTrp: 0.56 ± 0.024	1.225GlnTyr: 1.225 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.103ArgAla: 3.103 ± 0.053	0.365ArgCys: 0.365 ± 0.02	2.358ArgAsp: 2.358 ± 0.05	3.966ArgGlu: 3.966 ± 0.061	2.269ArgPhe: 2.269 ± 0.048	2.858ArgGly: 2.858 ± 0.05	1.105ArgHis: 1.105 ± 0.034	3.165ArgIle: 3.165 ± 0.051	3.3ArgLys: 3.3 ± 0.053	5.061ArgLeu: 5.061 ± 0.066	1.559ArgMet: 1.559 ± 0.039	1.766ArgAsn: 1.766 ± 0.041	1.684ArgPro: 1.684 ± 0.041	2.023ArgGln: 2.023 ± 0.039	2.405ArgArg: 2.405 ± 0.053	2.603ArgSer: 2.603 ± 0.049	2.487ArgThr: 2.487 ± 0.051	3.138ArgVal: 3.138 ± 0.05	0.591ArgTrp: 0.591 ± 0.021	1.916ArgTyr: 1.916 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.444SerAla: 3.444 ± 0.055	0.42SerCys: 0.42 ± 0.019	2.634SerAsp: 2.634 ± 0.047	3.784SerGlu: 3.784 ± 0.057	3.296SerPhe: 3.296 ± 0.06	4.112SerGly: 4.112 ± 0.061	1.33SerHis: 1.33 ± 0.04	4.434SerIle: 4.434 ± 0.065	2.822SerLys: 2.822 ± 0.051	6.116SerLeu: 6.116 ± 0.081	1.761SerMet: 1.761 ± 0.04	1.945SerAsn: 1.945 ± 0.039	2.201SerPro: 2.201 ± 0.044	1.91SerGln: 1.91 ± 0.043	2.508SerArg: 2.508 ± 0.047	3.632SerSer: 3.632 ± 0.061	2.917SerThr: 2.917 ± 0.045	4.121SerVal: 4.121 ± 0.059	0.711SerTrp: 0.711 ± 0.024	2.187SerTyr: 2.187 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	3.877ThrAla: 3.877 ± 0.066	0.422ThrCys: 0.422 ± 0.021	2.721ThrAsp: 2.721 ± 0.049	3.72ThrGlu: 3.72 ± 0.059	3.046ThrPhe: 3.046 ± 0.049	4.179ThrGly: 4.179 ± 0.069	1.108ThrHis: 1.108 ± 0.031	4.546ThrIle: 4.546 ± 0.071	3.066ThrLys: 3.066 ± 0.055	5.681ThrLeu: 5.681 ± 0.075	1.458ThrMet: 1.458 ± 0.04	2.289ThrAsn: 2.289 ± 0.046	2.304ThrPro: 2.304 ± 0.048	1.406ThrGln: 1.406 ± 0.035	2.056ThrArg: 2.056 ± 0.037	3.238ThrSer: 3.238 ± 0.056	3.087ThrThr: 3.087 ± 0.049	4.682ThrVal: 4.682 ± 0.065	0.59ThrTrp: 0.59 ± 0.023	2.103ThrTyr: 2.103 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.921ValAla: 5.921 ± 0.07	0.599ValCys: 0.599 ± 0.023	3.978ValAsp: 3.978 ± 0.066	5.619ValGlu: 5.619 ± 0.08	3.163ValPhe: 3.163 ± 0.058	5.128ValGly: 5.128 ± 0.088	1.703ValHis: 1.703 ± 0.042	5.619ValIle: 5.619 ± 0.072	4.264ValLys: 4.264 ± 0.065	7.077ValLeu: 7.077 ± 0.096	1.959ValMet: 1.959 ± 0.041	2.871ValAsn: 2.871 ± 0.051	2.916ValPro: 2.916 ± 0.047	2.727ValGln: 2.727 ± 0.055	3.245ValArg: 3.245 ± 0.057	4.538ValSer: 4.538 ± 0.058	4.727ValThr: 4.727 ± 0.072	5.674ValVal: 5.674 ± 0.07	0.697ValTrp: 0.697 ± 0.025	2.312ValTyr: 2.312 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.716TrpAla: 0.716 ± 0.028	0.091TrpCys: 0.091 ± 0.01	0.553TrpAsp: 0.553 ± 0.023	0.717TrpGlu: 0.717 ± 0.026	0.589TrpPhe: 0.589 ± 0.024	0.712TrpGly: 0.712 ± 0.024	0.257TrpHis: 0.257 ± 0.015	0.817TrpIle: 0.817 ± 0.032	0.731TrpLys: 0.731 ± 0.022	1.402TrpLeu: 1.402 ± 0.039	0.381TrpMet: 0.381 ± 0.017	0.521TrpAsn: 0.521 ± 0.022	0.325TrpPro: 0.325 ± 0.018	0.5TrpGln: 0.5 ± 0.02	0.507TrpArg: 0.507 ± 0.018	0.631TrpSer: 0.631 ± 0.023	0.622TrpThr: 0.622 ± 0.027	0.704TrpVal: 0.704 ± 0.028	0.163TrpTrp: 0.163 ± 0.013	0.375TrpTyr: 0.375 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.135TyrAla: 2.135 ± 0.041	0.301TyrCys: 0.301 ± 0.016	1.995TyrAsp: 1.995 ± 0.041	2.8TyrGlu: 2.8 ± 0.054	1.641TyrPhe: 1.641 ± 0.04	2.566TyrGly: 2.566 ± 0.05	0.938TyrHis: 0.938 ± 0.028	1.861TyrIle: 1.861 ± 0.038	1.521TyrLys: 1.521 ± 0.037	3.313TyrLeu: 3.313 ± 0.055	0.777TyrMet: 0.777 ± 0.022	1.145TyrAsn: 1.145 ± 0.034	1.415TyrPro: 1.415 ± 0.041	1.523TyrGln: 1.523 ± 0.04	1.809TyrArg: 1.809 ± 0.04	1.833TyrSer: 1.833 ± 0.042	1.755TyrThr: 1.755 ± 0.04	2.539TyrVal: 2.539 ± 0.052	0.43TyrTrp: 0.43 ± 0.019	1.309TyrTyr: 1.309 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4006 proteins (1169503 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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