Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Scheffersomyces; Scheffersomyces stipitis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3LZS4|A3LZS4_PICST Uncharacterized protein OS=Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) OX=322104 GN=PICST_33652 PE=4 SV=2
MM1 pKa = 7.7LLFQLIFAATALSSAVNWKK20 pKa = 9.68RR21 pKa = 11.84ASPFGDD27 pKa = 3.2FTGSNCNSACVNAGKK42 pKa = 8.26TLANSCAKK50 pKa = 9.84EE51 pKa = 4.02LQDD54 pKa = 3.22TTDD57 pKa = 3.18YY58 pKa = 11.26VSYY61 pKa = 10.7VKK63 pKa = 10.08CFCNQNSGFWDD74 pKa = 3.57NVAKK78 pKa = 10.42CDD80 pKa = 4.31CADD83 pKa = 3.8GANSGLTGSEE93 pKa = 3.72LRR95 pKa = 11.84AQICEE100 pKa = 3.9DD101 pKa = 3.34SGIFSALSEE110 pKa = 4.22FSDD113 pKa = 4.04LSINFSLTDD122 pKa = 3.81SLDD125 pKa = 3.45LTATNEE131 pKa = 4.12NTDD134 pKa = 3.21ATEE137 pKa = 4.11TNEE140 pKa = 4.28TDD142 pKa = 5.58DD143 pKa = 5.16SDD145 pKa = 4.02TDD147 pKa = 3.67SDD149 pKa = 4.11TGTGTAEE156 pKa = 4.16DD157 pKa = 4.76TDD159 pKa = 4.15TGTSGSGTAKK169 pKa = 10.48SSGSGTKK176 pKa = 10.11DD177 pKa = 2.94SSGSGTTTSTGTSATSGSTTTTRR200 pKa = 11.84TTGTSATSEE209 pKa = 4.47SEE211 pKa = 4.11TSATTNGAKK220 pKa = 10.5GLTCGISLYY229 pKa = 11.0LLALFILL236 pKa = 4.62

Molecular weight: 24.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3LZG0|A3LZG0_PICST Uncharacterized protein OS=Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) OX=322104 GN=PICST_37052 PE=4 SV=2
SS1 pKa = 7.07SPLQTFMGLVQRR13 pKa = 11.84RR14 pKa = 11.84FKK16 pKa = 11.25SRR18 pKa = 11.84GNTYY22 pKa = 10.24QPSTLKK28 pKa = 10.47RR29 pKa = 11.84KK30 pKa = 8.63RR31 pKa = 11.84TFGFLARR38 pKa = 11.84LRR40 pKa = 11.84TKK42 pKa = 10.4NGRR45 pKa = 11.84KK46 pKa = 8.86ILSRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 10.11AKK54 pKa = 9.98GRR56 pKa = 11.84WYY58 pKa = 9.08LTHH61 pKa = 7.23

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5797

0

5797

2852044

49

4979

492.0

55.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.88 ± 0.029

1.047 ± 0.01

5.927 ± 0.024

6.64 ± 0.028

4.561 ± 0.021

5.253 ± 0.031

2.116 ± 0.014

6.64 ± 0.024

6.855 ± 0.028

9.423 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.779 ± 0.01

5.747 ± 0.032

4.387 ± 0.029

3.81 ± 0.023

4.244 ± 0.018

9.298 ± 0.042

5.687 ± 0.036

6.127 ± 0.021

1.028 ± 0.009

3.551 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski