Torque teno virus (isolate Human/Germany/KAV/2001) (TTV)
Average proteome isoelectric point is 9.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q914N3|ORF24_TTVV4 Uncharacterized ORF2/4 protein OS=Torque teno virus (isolate Human/Germany/KAV/2001) OX=687345 GN=ORF2/4 PE=4 SV=2
MM1 pKa = 7.29 SWRR4 pKa = 11.84 PPAHH8 pKa = 5.89 NAEE11 pKa = 4.26 GQEE14 pKa = 4.2 GLWYY18 pKa = 10.43 RR19 pKa = 11.84 SVCQSHH25 pKa = 6.52 GAFCGCGDD33 pKa = 4.29 FVGHH37 pKa = 6.84 LNRR40 pKa = 11.84 LAEE43 pKa = 4.16 RR44 pKa = 11.84 LGRR47 pKa = 11.84 PQPPRR52 pKa = 11.84 PPGGPPGPAIRR63 pKa = 11.84 VLPALPPAEE72 pKa = 4.22 GSPRR76 pKa = 11.84 RR77 pKa = 11.84 HH78 pKa = 5.71 NRR80 pKa = 11.84 TEE82 pKa = 3.76 NQPCGGGDD90 pKa = 3.75 ADD92 pKa = 6.18 FGDD95 pKa = 4.06 QRR97 pKa = 11.84 DD98 pKa = 3.95 AGDD101 pKa = 3.84 GGAAADD107 pKa = 4.57 DD108 pKa = 4.56 LSPADD113 pKa = 5.19 VEE115 pKa = 6.18 DD116 pKa = 4.4 LLDD119 pKa = 3.83 WLDD122 pKa = 3.89 APDD125 pKa = 4.66 RR126 pKa = 11.84 LSKK129 pKa = 10.08 TPVNNPPLTSPVPVEE144 pKa = 4.35 SLVQYY149 pKa = 10.73 KK150 pKa = 10.69 LSTRR154 pKa = 11.84 STSTRR159 pKa = 11.84 ASRR162 pKa = 11.84 STDD165 pKa = 2.79 GTSDD169 pKa = 3.4 VASLGRR175 pKa = 11.84 QLLKK179 pKa = 10.45 EE180 pKa = 4.35 CKK182 pKa = 9.66 QNQQMLSFLQQVQNAQEE199 pKa = 4.14 QRR201 pKa = 11.84 SPWPLQEE208 pKa = 3.98 TPYY211 pKa = 11.19 LPGRR215 pKa = 11.84 EE216 pKa = 3.84 NAKK219 pKa = 10.02 PGKK222 pKa = 8.17 TRR224 pKa = 11.84 PRR226 pKa = 11.84 KK227 pKa = 9.73 KK228 pKa = 9.9 PRR230 pKa = 11.84 AKK232 pKa = 10.02 QKK234 pKa = 7.95 PKK236 pKa = 9.71 KK237 pKa = 8.65 RR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 11.84 YY241 pKa = 8.69 RR242 pKa = 11.84 SSSNSSSKK250 pKa = 11.45 SNDD253 pKa = 2.98 SSDD256 pKa = 4.32 AEE258 pKa = 4.37 SSTCSSNN265 pKa = 3.15
Molecular weight: 28.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.918
IPC2_protein 8.595
IPC_protein 8.58
Toseland 9.297
ProMoST 9.107
Dawson 9.56
Bjellqvist 9.428
Wikipedia 9.692
Rodwell 9.823
Grimsley 9.56
Solomon 9.663
Lehninger 9.633
Nozaki 9.677
DTASelect 9.297
Thurlkill 9.472
EMBOSS 9.765
Sillero 9.633
Patrickios 5.664
IPC_peptide 9.648
IPC2_peptide 8.302
IPC2.peptide.svr19 7.774
Protein with the highest isoelectric point:
>sp|Q914N2|ORF23_TTVV4 Probable protein VP2 OS=Torque teno virus (isolate Human/Germany/KAV/2001) OX=687345 GN=ORF2/3 PE=3 SV=2
MM1 pKa = 6.56 WWWRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 FWRR10 pKa = 11.84 PKK12 pKa = 10.04 RR13 pKa = 11.84 RR14 pKa = 11.84 WRR16 pKa = 11.84 WRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 PLARR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PARR34 pKa = 11.84 LARR37 pKa = 11.84 RR38 pKa = 11.84 PRR40 pKa = 11.84 VRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 FQWGRR50 pKa = 11.84 PRR52 pKa = 11.84 RR53 pKa = 11.84 YY54 pKa = 8.45 RR55 pKa = 11.84 PRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 KK64 pKa = 8.28 RR65 pKa = 11.84 QKK67 pKa = 10.29 IKK69 pKa = 10.68 LKK71 pKa = 9.32 QWNPAVTKK79 pKa = 8.01 FCRR82 pKa = 11.84 IIGYY86 pKa = 9.41 YY87 pKa = 9.57 PLIICGEE94 pKa = 4.23 GTTVRR99 pKa = 11.84 NYY101 pKa = 10.24 NVHH104 pKa = 5.41 SQDD107 pKa = 3.31 YY108 pKa = 8.83 VQYY111 pKa = 8.72 EE112 pKa = 4.59 SYY114 pKa = 10.78 GGGMTTTMFNLKK126 pKa = 9.81 VPYY129 pKa = 9.89 EE130 pKa = 3.94 QFQLHH135 pKa = 4.96 QNFWTRR141 pKa = 11.84 SNIDD145 pKa = 3.46 LDD147 pKa = 3.7 LVRR150 pKa = 11.84 YY151 pKa = 9.61 HH152 pKa = 6.22 SVKK155 pKa = 9.47 FTFFRR160 pKa = 11.84 HH161 pKa = 5.67 PKK163 pKa = 9.26 VDD165 pKa = 5.0 FIVKK169 pKa = 9.51 FNRR172 pKa = 11.84 NPPFTDD178 pKa = 3.3 SVLTGPMLHH187 pKa = 7.03 PGVLMNSKK195 pKa = 10.28 RR196 pKa = 11.84 KK197 pKa = 9.61 ILIPSWEE204 pKa = 3.93 TRR206 pKa = 11.84 PRR208 pKa = 11.84 GKK210 pKa = 10.24 KK211 pKa = 9.69 RR212 pKa = 11.84 IPVRR216 pKa = 11.84 IGPPRR221 pKa = 11.84 LFTDD225 pKa = 3.16 HH226 pKa = 7.43 WYY228 pKa = 9.49 FQRR231 pKa = 11.84 DD232 pKa = 3.74 FCGVPLLTVNATACNLRR249 pKa = 11.84 YY250 pKa = 9.55 PFGSPQTKK258 pKa = 10.13 NICIYY263 pKa = 10.38 FLVLASIYY271 pKa = 10.01 NDD273 pKa = 3.11 KK274 pKa = 11.14 VSIAKK279 pKa = 10.52 DD280 pKa = 3.64 EE281 pKa = 4.18 VTKK284 pKa = 11.05 NYY286 pKa = 7.73 DD287 pKa = 3.64 TLVDD291 pKa = 5.16 AIKK294 pKa = 10.5 KK295 pKa = 9.48 SPQGLTVFNTFKK307 pKa = 10.01 TQEE310 pKa = 4.01 HH311 pKa = 4.86 MQEE314 pKa = 4.36 PSSEE318 pKa = 4.39 TCKK321 pKa = 9.6 EE322 pKa = 4.38 TQKK325 pKa = 9.91 TNPPFKK331 pKa = 10.88 YY332 pKa = 10.25 EE333 pKa = 4.09 NLSSLWGDD341 pKa = 4.04 KK342 pKa = 10.04 IYY344 pKa = 11.03 KK345 pKa = 10.08 EE346 pKa = 4.14 STFNSFKK353 pKa = 10.92 TNAEE357 pKa = 4.22 NLWKK361 pKa = 10.51 ARR363 pKa = 11.84 SKK365 pKa = 9.77 HH366 pKa = 4.97 TLLGSKK372 pKa = 9.21 EE373 pKa = 4.47 LNFKK377 pKa = 9.69 TGMYY381 pKa = 10.13 SAIFLTNGRR390 pKa = 11.84 LSPDD394 pKa = 3.12 YY395 pKa = 9.85 PGIYY399 pKa = 9.23 IEE401 pKa = 4.74 VVYY404 pKa = 11.01 NPLLGQGEE412 pKa = 4.99 GNMIWLEE419 pKa = 3.98 WCPKK423 pKa = 8.68 KK424 pKa = 9.34 TKK426 pKa = 10.13 QYY428 pKa = 11.25 NEE430 pKa = 3.97 RR431 pKa = 11.84 QCYY434 pKa = 10.17 NLIQHH439 pKa = 5.7 VPLWAAIHH447 pKa = 6.72 GYY449 pKa = 9.27 MDD451 pKa = 3.98 FCKK454 pKa = 10.01 KK455 pKa = 8.21 TFKK458 pKa = 11.05 DD459 pKa = 3.46 NNLDD463 pKa = 3.38 KK464 pKa = 10.24 NTRR467 pKa = 11.84 VVLICPYY474 pKa = 9.74 TRR476 pKa = 11.84 PQLYY480 pKa = 10.16 NPQKK484 pKa = 9.84 PDD486 pKa = 2.84 WGYY489 pKa = 11.21 VPYY492 pKa = 10.18 DD493 pKa = 3.51 YY494 pKa = 11.69 NFAQTKK500 pKa = 8.88 MPDD503 pKa = 3.2 GNGYY507 pKa = 9.44 IPEE510 pKa = 4.7 FYY512 pKa = 10.42 RR513 pKa = 11.84 FRR515 pKa = 11.84 WYY517 pKa = 10.0 PSGFHH522 pKa = 5.19 QQNWMNDD529 pKa = 3.26 LDD531 pKa = 3.84 QCGPFSHH538 pKa = 7.11 RR539 pKa = 11.84 GEE541 pKa = 4.17 EE542 pKa = 4.19 KK543 pKa = 10.18 MATLTSKK550 pKa = 11.37 YY551 pKa = 10.07 NFKK554 pKa = 9.65 FTWGGNPIFQQTVKK568 pKa = 10.66 DD569 pKa = 3.69 PCKK572 pKa = 10.37 QSTFDD577 pKa = 3.35 IPGAGGIPRR586 pKa = 11.84 PIQIVNPKK594 pKa = 9.72 YY595 pKa = 10.3 LDD597 pKa = 3.72 PGITFHH603 pKa = 7.39 RR604 pKa = 11.84 WDD606 pKa = 3.5 LRR608 pKa = 11.84 RR609 pKa = 11.84 GFFGPAAVKK618 pKa = 10.38 RR619 pKa = 11.84 MQAEE623 pKa = 4.19 STDD626 pKa = 3.57 ALFPTTGPKK635 pKa = 9.84 RR636 pKa = 11.84 PRR638 pKa = 11.84 TEE640 pKa = 3.62 VPVAVAGDD648 pKa = 3.89 ALPSRR653 pKa = 11.84 EE654 pKa = 4.25 RR655 pKa = 11.84 KK656 pKa = 8.46 RR657 pKa = 11.84 QAWQDD662 pKa = 3.4 STSEE666 pKa = 4.14 EE667 pKa = 4.42 TEE669 pKa = 4.25 SEE671 pKa = 4.3 AEE673 pKa = 3.99 AQEE676 pKa = 4.25 EE677 pKa = 4.36 KK678 pKa = 10.28 TLQEE682 pKa = 4.12 QLQQQLKK689 pKa = 7.19 EE690 pKa = 4.08 QRR692 pKa = 11.84 QLRR695 pKa = 11.84 CGIQYY700 pKa = 7.87 MFQQLTKK707 pKa = 8.95 TQLHH711 pKa = 5.87 LHH713 pKa = 6.14 VPPIPQQ719 pKa = 3.2
Molecular weight: 85.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.288
IPC2_protein 9.604
IPC_protein 9.911
Toseland 10.277
ProMoST 9.955
Dawson 10.452
Bjellqvist 10.145
Wikipedia 10.628
Rodwell 10.818
Grimsley 10.526
Solomon 10.482
Lehninger 10.452
Nozaki 10.277
DTASelect 10.131
Thurlkill 10.306
EMBOSS 10.672
Sillero 10.365
Patrickios 10.394
IPC_peptide 10.482
IPC2_peptide 9.004
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1258
265
719
419.3
48.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.041 ± 1.842
1.908 ± 0.118
5.008 ± 0.856
4.452 ± 0.03
4.054 ± 1.186
6.836 ± 0.98
1.908 ± 0.153
2.941 ± 1.281
5.644 ± 1.321
7.154 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.669 ± 0.307
4.849 ± 0.229
9.777 ± 1.577
5.882 ± 0.375
10.413 ± 0.661
5.882 ± 1.838
5.564 ± 0.855
4.054 ± 0.441
2.623 ± 0.401
3.339 ± 1.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here