Munguba virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Munguba phlebovirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1T343|I1T343_9VIRU Nonstructural protein OS=Munguba virus OX=1048854 PE=4 SV=1
MM1 pKa = 7.37SFLWNLPVVRR11 pKa = 11.84PSSCFLGLPSVNYY24 pKa = 10.0VAFNKK29 pKa = 9.24MATFEE34 pKa = 4.11ACKK37 pKa = 10.79VNGAEE42 pKa = 5.15IPLKK46 pKa = 10.34HH47 pKa = 6.8LKK49 pKa = 10.06LCYY52 pKa = 9.94DD53 pKa = 3.49KK54 pKa = 11.22RR55 pKa = 11.84PTLFDD60 pKa = 3.49FLRR63 pKa = 11.84NRR65 pKa = 11.84EE66 pKa = 4.2VPVSWGDD73 pKa = 3.48HH74 pKa = 5.87TDD76 pKa = 3.54DD77 pKa = 4.74QILNRR82 pKa = 11.84SPRR85 pKa = 11.84TFEE88 pKa = 3.79GLMFGLSEE96 pKa = 4.14IEE98 pKa = 3.79EE99 pKa = 4.39VEE101 pKa = 3.92YY102 pKa = 11.2GRR104 pKa = 11.84YY105 pKa = 9.02NEE107 pKa = 4.8PYY109 pKa = 9.96LKK111 pKa = 10.55KK112 pKa = 10.46AVSWPLSYY120 pKa = 10.31PSHH123 pKa = 6.11TFFNLAVRR131 pKa = 11.84KK132 pKa = 8.16TEE134 pKa = 3.75MGNWLHH140 pKa = 7.04SSMWATMMLRR150 pKa = 11.84MSHH153 pKa = 6.01TDD155 pKa = 3.12NLEE158 pKa = 3.52EE159 pKa = 4.96SIIKK163 pKa = 8.91VRR165 pKa = 11.84KK166 pKa = 9.81RR167 pKa = 11.84IIEE170 pKa = 4.05KK171 pKa = 10.71ANDD174 pKa = 3.32MGLDD178 pKa = 3.7LSLFTGEE185 pKa = 4.39NLILEE190 pKa = 4.57IAHH193 pKa = 6.04LQCLRR198 pKa = 11.84LIYY201 pKa = 10.32ASRR204 pKa = 11.84KK205 pKa = 6.85EE206 pKa = 3.87RR207 pKa = 11.84EE208 pKa = 3.77RR209 pKa = 11.84GVNHH213 pKa = 6.65SKK215 pKa = 9.48TYY217 pKa = 10.4SIVRR221 pKa = 11.84SYY223 pKa = 11.89DD224 pKa = 3.47CFSVISDD231 pKa = 3.65VNFDD235 pKa = 3.72PMIADD240 pKa = 3.87SLFGSNDD247 pKa = 2.61GTMSGEE253 pKa = 3.97SDD255 pKa = 3.18VDD257 pKa = 3.41QLRR260 pKa = 11.84VDD262 pKa = 3.92LEE264 pKa = 4.66RR265 pKa = 11.84YY266 pKa = 9.71LSLL269 pKa = 5.29

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1T343|I1T343_9VIRU Nonstructural protein OS=Munguba virus OX=1048854 PE=4 SV=1
MM1 pKa = 7.2SADD4 pKa = 3.58YY5 pKa = 10.81QRR7 pKa = 11.84IAIEE11 pKa = 4.55FGSEE15 pKa = 3.99PIDD18 pKa = 3.8RR19 pKa = 11.84DD20 pKa = 5.19AITLWVNEE28 pKa = 3.91FAYY31 pKa = 10.56QGFDD35 pKa = 2.87ASNVLRR41 pKa = 11.84LLQQRR46 pKa = 11.84GGATWKK52 pKa = 10.48EE53 pKa = 4.07DD54 pKa = 3.36AKK56 pKa = 11.53KK57 pKa = 10.17MIILVLTRR65 pKa = 11.84GNKK68 pKa = 8.28PEE70 pKa = 3.96NMVKK74 pKa = 10.29RR75 pKa = 11.84MSEE78 pKa = 3.59AGKK81 pKa = 8.65RR82 pKa = 11.84TVEE85 pKa = 3.96ALVVKK90 pKa = 10.05YY91 pKa = 8.12QLKK94 pKa = 10.21SGNPGRR100 pKa = 11.84DD101 pKa = 3.51DD102 pKa = 3.36LTLARR107 pKa = 11.84VAAALAPWTCQAAFVVSEE125 pKa = 4.16FMPVTGRR132 pKa = 11.84AMDD135 pKa = 3.93DD136 pKa = 3.34HH137 pKa = 6.5AASYY141 pKa = 9.17PRR143 pKa = 11.84GMMHH147 pKa = 6.83TSFAGLIDD155 pKa = 3.56PTLPQSTYY163 pKa = 9.61SDD165 pKa = 3.49LVAAHH170 pKa = 6.89SLFLYY175 pKa = 10.08YY176 pKa = 10.47FSKK179 pKa = 9.82TINVGLRR186 pKa = 11.84GKK188 pKa = 8.6TKK190 pKa = 11.0AEE192 pKa = 3.97VEE194 pKa = 4.26ASFSQPMQAAINSGFISGSQRR215 pKa = 11.84RR216 pKa = 11.84QFLQNLGIIDD226 pKa = 4.18RR227 pKa = 11.84NSVAVPAVATAAAAYY242 pKa = 7.98RR243 pKa = 11.84ALSS246 pKa = 3.45

Molecular weight: 26.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4023

246

2090

1005.8

113.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.792 ± 1.443

2.784 ± 0.702

5.667 ± 0.486

6.513 ± 0.436

4.599 ± 0.317

5.841 ± 0.447

2.337 ± 0.381

6.985 ± 0.615

6.637 ± 0.537

8.949 ± 0.651

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.68

3.853 ± 0.373

3.604 ± 0.278

3.082 ± 0.546

5.319 ± 0.377

10.241 ± 0.548

4.822 ± 0.205

5.866 ± 0.373

1.193 ± 0.146

2.784 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski