Cyclovirus PK5034

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Human associated cyclovirus 5

Average proteome isoelectric point is 8.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4N3N9|D4N3N9_9CIRC Capsid protein OS=Cyclovirus PK5034 OX=742916 GN=cap PE=4 SV=1
MM1 pKa = 7.38NSTVRR6 pKa = 11.84RR7 pKa = 11.84FCFTWNNYY15 pKa = 7.15TVADD19 pKa = 3.98TLTVKK24 pKa = 10.59DD25 pKa = 4.44YY26 pKa = 10.5FVKK29 pKa = 9.55YY30 pKa = 9.51CKK32 pKa = 10.39YY33 pKa = 10.33GIAGEE38 pKa = 4.11EE39 pKa = 4.07LAPEE43 pKa = 4.43TGTPHH48 pKa = 6.9LQGFCNLRR56 pKa = 11.84KK57 pKa = 8.63PQRR60 pKa = 11.84FSAIKK65 pKa = 9.62KK66 pKa = 8.32HH67 pKa = 5.81LSDD70 pKa = 5.57RR71 pKa = 11.84IHH73 pKa = 6.88IEE75 pKa = 3.68KK76 pKa = 10.95ANGSDD81 pKa = 3.55EE82 pKa = 4.44QNQSYY87 pKa = 9.98CKK89 pKa = 10.27KK90 pKa = 9.15AGHH93 pKa = 6.4WFEE96 pKa = 4.06QGVPVKK102 pKa = 10.72QGDD105 pKa = 3.7RR106 pKa = 11.84SDD108 pKa = 3.85LKK110 pKa = 11.19SVVSTIADD118 pKa = 3.82GANTAQAIAEE128 pKa = 4.11KK129 pKa = 10.58HH130 pKa = 4.9PVEE133 pKa = 4.68FIKK136 pKa = 10.72YY137 pKa = 7.94YY138 pKa = 10.67KK139 pKa = 10.42GISEE143 pKa = 4.51YY144 pKa = 10.97LRR146 pKa = 11.84LLAPIAPRR154 pKa = 11.84KK155 pKa = 9.1FKK157 pKa = 10.61TYY159 pKa = 10.07VYY161 pKa = 9.24YY162 pKa = 10.7YY163 pKa = 8.96WGPPGSGKK171 pKa = 8.76SRR173 pKa = 11.84RR174 pKa = 11.84ALEE177 pKa = 4.15EE178 pKa = 4.36AEE180 pKa = 4.75SLDD183 pKa = 4.11PDD185 pKa = 4.08SIYY188 pKa = 10.95YY189 pKa = 9.76KK190 pKa = 10.51PRR192 pKa = 11.84GLWWDD197 pKa = 4.51GYY199 pKa = 10.2RR200 pKa = 11.84QQQSVIIDD208 pKa = 4.02DD209 pKa = 4.4FYY211 pKa = 11.87GWIKK215 pKa = 10.75YY216 pKa = 10.28DD217 pKa = 3.96EE218 pKa = 4.46LLKK221 pKa = 10.08ITDD224 pKa = 4.0RR225 pKa = 11.84YY226 pKa = 8.69PYY228 pKa = 9.84KK229 pKa = 10.71VQVKK233 pKa = 10.29GGFEE237 pKa = 4.32EE238 pKa = 4.88FTSTRR243 pKa = 11.84IWFTSNVDD251 pKa = 2.95TDD253 pKa = 3.21RR254 pKa = 11.84LYY256 pKa = 11.08RR257 pKa = 11.84FDD259 pKa = 4.42GFTPEE264 pKa = 3.71AFEE267 pKa = 4.54RR268 pKa = 11.84RR269 pKa = 11.84ITAKK273 pKa = 10.14IHH275 pKa = 4.58ITT277 pKa = 3.41

Molecular weight: 32.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4N3N9|D4N3N9_9CIRC Capsid protein OS=Cyclovirus PK5034 OX=742916 GN=cap PE=4 SV=1
MM1 pKa = 7.79AYY3 pKa = 9.97RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VYY9 pKa = 9.56RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.04PVRR15 pKa = 11.84RR16 pKa = 11.84LGRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84VYY24 pKa = 9.8RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 4.88RR29 pKa = 11.84RR30 pKa = 11.84SAKK33 pKa = 10.13GKK35 pKa = 9.9LLAKK39 pKa = 8.16FTKK42 pKa = 9.01VTLLTVEE49 pKa = 4.57HH50 pKa = 7.2KK51 pKa = 9.69STSVWPVSFKK61 pKa = 11.4CNDD64 pKa = 3.0FKK66 pKa = 11.31EE67 pKa = 4.35FEE69 pKa = 4.08RR70 pKa = 11.84LKK72 pKa = 10.48TSFEE76 pKa = 4.11YY77 pKa = 10.28IKK79 pKa = 10.24FYY81 pKa = 11.07KK82 pKa = 10.23VVATVYY88 pKa = 10.26PLQNVSNNSTSMCPCYY104 pKa = 11.01AMAPWHH110 pKa = 6.16TSIAMPKK117 pKa = 10.27DD118 pKa = 3.54FEE120 pKa = 6.34AYY122 pKa = 9.11MSLDD126 pKa = 3.14RR127 pKa = 11.84AKK129 pKa = 9.93MYY131 pKa = 10.6RR132 pKa = 11.84QTQVGRR138 pKa = 11.84QVYY141 pKa = 9.04VPSTQLVTDD150 pKa = 4.14WKK152 pKa = 11.1SNEE155 pKa = 3.66TSYY158 pKa = 11.67GQVQWRR164 pKa = 11.84PEE166 pKa = 4.22LKK168 pKa = 10.42CNLIGTQEE176 pKa = 4.29HH177 pKa = 7.15PNIQCGAVAFQGNLGATDD195 pKa = 3.5TKK197 pKa = 11.14SYY199 pKa = 10.95FSIKK203 pKa = 10.25LDD205 pKa = 3.68VYY207 pKa = 11.16CKK209 pKa = 10.75LKK211 pKa = 10.51GQNTMIVV218 pKa = 3.17

Molecular weight: 25.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

495

218

277

247.5

28.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.859 ± 0.234

2.02 ± 0.484

4.646 ± 1.252

5.051 ± 0.913

4.848 ± 0.476

5.657 ± 1.01

2.02 ± 0.122

4.848 ± 1.385

8.485 ± 0.152

5.859 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 1.054

3.434 ± 0.459

4.444 ± 0.209

4.444 ± 0.397

8.081 ± 1.329

6.263 ± 0.409

6.869 ± 0.311

6.667 ± 1.354

2.222 ± 0.256

6.667 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski