Microviridae IME-16

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088NCE2|A0A088NCE2_9VIRU Replication initiator OS=Microviridae IME-16 OX=1544364 GN=IME-002 PE=4 SV=1
MM1 pKa = 6.97FTRR4 pKa = 11.84VVPPVPVEE12 pKa = 4.03VQDD15 pKa = 4.0VVIKK19 pKa = 10.76RR20 pKa = 11.84PVEE23 pKa = 3.99VVTDD27 pKa = 3.45NGVDD31 pKa = 2.95IRR33 pKa = 11.84YY34 pKa = 7.59TDD36 pKa = 3.68KK37 pKa = 11.59EE38 pKa = 4.24EE39 pKa = 4.36ALPPYY44 pKa = 10.18DD45 pKa = 3.77SYY47 pKa = 12.0SVEE50 pKa = 4.22SLSAAGVPLKK60 pKa = 10.66AVNPAILDD68 pKa = 3.99SVDD71 pKa = 3.18VAAVNEE77 pKa = 4.47FVSRR81 pKa = 11.84EE82 pKa = 3.81SAKK85 pKa = 10.78AEE87 pKa = 3.8KK88 pKa = 10.78AEE90 pKa = 4.2FSSTVVEE97 pKa = 5.05DD98 pKa = 3.65NTSTSQTEE106 pKa = 4.01PVTNSS111 pKa = 2.72

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088NC48|A0A088NC48_9VIRU M16p1 OS=Microviridae IME-16 OX=1544364 GN=IME-001 PE=4 SV=1
MM1 pKa = 7.24FNVYY5 pKa = 9.8KK6 pKa = 9.96EE7 pKa = 4.02KK8 pKa = 10.89RR9 pKa = 11.84FIASFEE15 pKa = 4.0RR16 pKa = 11.84LKK18 pKa = 10.84DD19 pKa = 3.07ARR21 pKa = 11.84AYY23 pKa = 7.27VTSVLFSEE31 pKa = 4.99YY32 pKa = 10.16SRR34 pKa = 11.84KK35 pKa = 10.14GYY37 pKa = 10.5EE38 pKa = 4.2VVCVNFPRR46 pKa = 11.84RR47 pKa = 11.84GDD49 pKa = 3.41PYY51 pKa = 10.41IYY53 pKa = 10.34TFGKK57 pKa = 10.27KK58 pKa = 10.13GICKK62 pKa = 9.4VVTIYY67 pKa = 10.71RR68 pKa = 11.84VLL70 pKa = 3.64

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1714

39

706

244.9

27.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.709 ± 1.128

1.634 ± 0.517

7.176 ± 0.562

3.501 ± 0.964

5.776 ± 0.81

4.551 ± 0.738

1.692 ± 0.3

5.309 ± 0.361

6.184 ± 0.894

8.81 ± 0.888

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.575 ± 0.201

5.951 ± 0.523

4.434 ± 0.438

2.217 ± 0.534

4.842 ± 0.363

11.085 ± 1.116

5.543 ± 0.414

6.943 ± 1.399

0.467 ± 0.123

5.601 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski