Heterobasidion partitivirus 13

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8PA42|W8PA42_9VIRU RNA-dependent RNA polymerase OS=Heterobasidion partitivirus 13 OX=1469906 PE=4 SV=1
MM1 pKa = 7.79PSYY4 pKa = 10.17TFDD7 pKa = 3.79PVSVMSMNEE16 pKa = 3.66TEE18 pKa = 4.28KK19 pKa = 11.25AKK21 pKa = 10.63FLADD25 pKa = 3.79LLEE28 pKa = 4.79GRR30 pKa = 11.84HH31 pKa = 5.07EE32 pKa = 4.31TQAPTKK38 pKa = 10.14SAEE41 pKa = 3.92QADD44 pKa = 4.7PIGARR49 pKa = 11.84PVVAQARR56 pKa = 11.84RR57 pKa = 11.84NVTAANSDD65 pKa = 3.85GPSKK69 pKa = 10.17LTYY72 pKa = 9.96TKK74 pKa = 10.47VKK76 pKa = 10.2PFTRR80 pKa = 11.84DD81 pKa = 2.7SDD83 pKa = 3.46RR84 pKa = 11.84DD85 pKa = 3.25AANAVRR91 pKa = 11.84LALGSSAFRR100 pKa = 11.84TVTHH104 pKa = 6.86FGLNTFIPDD113 pKa = 3.75FTLLFQALAALDD125 pKa = 3.97FAMLSTDD132 pKa = 3.45KK133 pKa = 10.68FCRR136 pKa = 11.84ASEE139 pKa = 4.19FWTPAVSRR147 pKa = 11.84IYY149 pKa = 10.31IAVICYY155 pKa = 7.42VQVMRR160 pKa = 11.84SMRR163 pKa = 11.84ATSTNFGVEE172 pKa = 3.74IQQFLDD178 pKa = 2.9WFEE181 pKa = 3.96EE182 pKa = 4.17HH183 pKa = 6.89FPLNTLPVPGPLVNFISTISSCHH206 pKa = 6.5ISTLDD211 pKa = 3.55YY212 pKa = 11.32KK213 pKa = 10.54VMCPILPRR221 pKa = 11.84LVPCAANGRR230 pKa = 11.84WVIHH234 pKa = 6.27NNLSLRR240 pKa = 11.84FPPVPTLFQQLSEE253 pKa = 4.43HH254 pKa = 6.16YY255 pKa = 10.47NYY257 pKa = 11.05ANPAAAAPQTAEE269 pKa = 4.7QIGRR273 pKa = 11.84WADD276 pKa = 3.44YY277 pKa = 11.01GRR279 pKa = 11.84TQYY282 pKa = 11.41GITAWNANAATSQTHH297 pKa = 5.27RR298 pKa = 11.84TTLTALSNATHH309 pKa = 6.98AYY311 pKa = 9.66AWAAPGNSTPYY322 pKa = 9.19RR323 pKa = 11.84TNTSVGRR330 pKa = 11.84NLIDD334 pKa = 3.19YY335 pKa = 9.54GMAMDD340 pKa = 4.99SLIPPEE346 pKa = 4.41FNSAAANGPAAANPTWQTYY365 pKa = 10.58LGFEE369 pKa = 4.52TTHH372 pKa = 4.89EE373 pKa = 4.23WFPEE377 pKa = 4.08FARR380 pKa = 11.84IMSVYY385 pKa = 10.5SKK387 pKa = 10.17FWKK390 pKa = 10.31EE391 pKa = 3.88STSLDD396 pKa = 3.62TISPVGSTACLAVAEE411 pKa = 4.51ATAQLVQPTTRR422 pKa = 11.84YY423 pKa = 7.99PALTLSRR430 pKa = 11.84NFVVRR435 pKa = 11.84ISQLPEE441 pKa = 4.87ADD443 pKa = 4.47LLDD446 pKa = 4.15SQLSAIHH453 pKa = 6.35CADD456 pKa = 3.4TGTVTAHH463 pKa = 7.31ASTPNVNVSTGPYY476 pKa = 9.57LDD478 pKa = 4.81LAIQEE483 pKa = 4.2RR484 pKa = 11.84ARR486 pKa = 11.84AVDD489 pKa = 3.16ASGGLRR495 pKa = 11.84QIIEE499 pKa = 4.57DD500 pKa = 4.31YY501 pKa = 11.36YY502 pKa = 11.22HH503 pKa = 5.67VQNPKK508 pKa = 10.42AA509 pKa = 3.57

Molecular weight: 55.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8PA42|W8PA42_9VIRU RNA-dependent RNA polymerase OS=Heterobasidion partitivirus 13 OX=1469906 PE=4 SV=1
MM1 pKa = 7.48LPIISQVTDD10 pKa = 2.99YY11 pKa = 11.25VYY13 pKa = 11.05RR14 pKa = 11.84KK15 pKa = 9.55FIKK18 pKa = 9.79PAPTQFKK25 pKa = 11.02NNYY28 pKa = 9.04LFQNWLSPLTTPHH41 pKa = 7.36RR42 pKa = 11.84DD43 pKa = 2.93ATKK46 pKa = 8.54YY47 pKa = 10.84AEE49 pKa = 4.03YY50 pKa = 10.08QAYY53 pKa = 9.87LEE55 pKa = 3.96KK56 pKa = 10.48HH57 pKa = 5.57IRR59 pKa = 11.84SNLLGSDD66 pKa = 3.24AEE68 pKa = 4.32YY69 pKa = 10.37VIKK72 pKa = 10.67RR73 pKa = 11.84FHH75 pKa = 6.73HH76 pKa = 6.99PIATIDD82 pKa = 3.86SVNEE86 pKa = 3.64TLQRR90 pKa = 11.84GDD92 pKa = 4.64LPDD95 pKa = 3.98HH96 pKa = 6.61PVPKK100 pKa = 10.1DD101 pKa = 3.24EE102 pKa = 4.57HH103 pKa = 6.75YY104 pKa = 11.17YY105 pKa = 10.39RR106 pKa = 11.84ALTEE110 pKa = 4.03TTKK113 pKa = 10.77RR114 pKa = 11.84FAPPQLIRR122 pKa = 11.84PIHH125 pKa = 5.51FADD128 pKa = 3.24LRR130 pKa = 11.84YY131 pKa = 10.25YY132 pKa = 8.96EE133 pKa = 4.82WNWHH137 pKa = 6.08PNVEE141 pKa = 4.18EE142 pKa = 4.71PYY144 pKa = 10.85VSNSQLKK151 pKa = 8.07TAVQDD156 pKa = 3.71AYY158 pKa = 10.93HH159 pKa = 6.96AGLLEE164 pKa = 4.77DD165 pKa = 3.92GRR167 pKa = 11.84MSFGNLKK174 pKa = 9.74NHH176 pKa = 5.22VFMDD180 pKa = 3.46VRR182 pKa = 11.84HH183 pKa = 5.5FLHH186 pKa = 7.02RR187 pKa = 11.84IKK189 pKa = 10.42RR190 pKa = 11.84GQISDD195 pKa = 3.89PHH197 pKa = 5.66TLWPLINMHH206 pKa = 6.27VKK208 pKa = 9.83PALTEE213 pKa = 3.82TDD215 pKa = 3.19TTKK218 pKa = 10.15IRR220 pKa = 11.84LVFGVSKK227 pKa = 10.55RR228 pKa = 11.84HH229 pKa = 4.51VLPRR233 pKa = 11.84AMFFWPLFRR242 pKa = 11.84YY243 pKa = 10.21YY244 pKa = 11.03LDD246 pKa = 4.45NRR248 pKa = 11.84DD249 pKa = 4.1KK250 pKa = 11.57SPMLWGFEE258 pKa = 4.25TILGGMMLLNSEE270 pKa = 4.44MLLSRR275 pKa = 11.84LYY277 pKa = 8.9YY278 pKa = 8.56QTFVTVDD285 pKa = 2.6WSGFDD290 pKa = 3.55LRR292 pKa = 11.84SLFSIIRR299 pKa = 11.84EE300 pKa = 4.27DD301 pKa = 4.29IFPAWRR307 pKa = 11.84TYY309 pKa = 10.78FDD311 pKa = 4.19FNNGYY316 pKa = 8.53MPTKK320 pKa = 10.26FYY322 pKa = 10.95KK323 pKa = 10.59SSTADD328 pKa = 3.37PDD330 pKa = 3.44QLEE333 pKa = 4.6RR334 pKa = 11.84LWNWTNEE341 pKa = 4.33AVFKK345 pKa = 10.79MPFRR349 pKa = 11.84TMDD352 pKa = 3.04GATFLRR358 pKa = 11.84LFRR361 pKa = 11.84GIPSGLFEE369 pKa = 4.32TQFLDD374 pKa = 3.42SFYY377 pKa = 11.35NMLMILTILDD387 pKa = 3.29AMGFDD392 pKa = 3.54ISTIYY397 pKa = 10.42IRR399 pKa = 11.84VQGDD403 pKa = 3.42DD404 pKa = 3.87SLLLLTFFLPADD416 pKa = 3.26QHH418 pKa = 8.68AEE420 pKa = 4.28FKK422 pKa = 11.15AQFEE426 pKa = 4.26ALAAYY431 pKa = 10.24YY432 pKa = 9.5FDD434 pKa = 4.17HH435 pKa = 7.29VARR438 pKa = 11.84QDD440 pKa = 3.22KK441 pKa = 10.5TDD443 pKa = 3.43ISNTSQNVAVLGYY456 pKa = 10.47SNDD459 pKa = 3.07NGYY462 pKa = 9.77PSRR465 pKa = 11.84DD466 pKa = 2.97WRR468 pKa = 11.84KK469 pKa = 9.46LLAQLFHH476 pKa = 7.04PRR478 pKa = 11.84SQRR481 pKa = 11.84PTLSLLKK488 pKa = 10.38ARR490 pKa = 11.84CCGIQYY496 pKa = 11.14ASMYY500 pKa = 10.13KK501 pKa = 9.84YY502 pKa = 10.17PQVTNVAKK510 pKa = 9.81ATFNQLDD517 pKa = 3.93SEE519 pKa = 4.94GVQPVKK525 pKa = 10.52LAAQRR530 pKa = 11.84DD531 pKa = 4.6VILHH535 pKa = 4.62SHH537 pKa = 6.75KK538 pKa = 10.81DD539 pKa = 3.64FYY541 pKa = 11.75VPTDD545 pKa = 3.53HH546 pKa = 7.39FPTLNEE552 pKa = 3.48VTRR555 pKa = 11.84YY556 pKa = 10.13LRR558 pKa = 11.84IPYY561 pKa = 8.48TRR563 pKa = 11.84TEE565 pKa = 4.31ADD567 pKa = 3.27SEE569 pKa = 4.79TYY571 pKa = 10.89YY572 pKa = 10.6PMSHH576 pKa = 7.34FLSQFF581 pKa = 3.01

Molecular weight: 68.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1090

509

581

545.0

62.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.266 ± 2.418

0.826 ± 0.359

5.505 ± 0.773

3.945 ± 0.01

5.872 ± 0.756

3.853 ± 0.178

3.119 ± 0.498

4.495 ± 0.113

3.394 ± 0.806

9.266 ± 0.92

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.335

4.679 ± 0.28

6.422 ± 0.297

4.22 ± 0.062

5.872 ± 0.371

6.697 ± 0.63

8.073 ± 0.887

5.413 ± 0.443

1.835 ± 0.044

4.771 ± 0.807
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski