Streptococcus satellite phage Javan328
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKI9|A0A4D5ZKI9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan328 OX=2558640 GN=JavanS328_0015 PE=4 SV=1
MM1 pKa = 6.29 TTKK4 pKa = 10.57 KK5 pKa = 10.43 AISSQQVLLSAKK17 pKa = 10.33 KK18 pKa = 8.96 LTEE21 pKa = 4.45 LGNEE25 pKa = 4.17 LTDD28 pKa = 3.22 IMNVLEE34 pKa = 4.34 MNNLALEE41 pKa = 4.35 GLEE44 pKa = 4.63 FALQNDD50 pKa = 3.86 TTTFLWLAKK59 pKa = 10.0 KK60 pKa = 9.12 YY61 pKa = 9.45 TDD63 pKa = 2.9 TAYY66 pKa = 10.61 AQNEE70 pKa = 4.14 KK71 pKa = 10.83 LYY73 pKa = 11.24 DD74 pKa = 3.88 RR75 pKa = 11.84 LNEE78 pKa = 4.03 IAFLLLNNDD87 pKa = 3.72 DD88 pKa = 4.25 AKK90 pKa = 10.99 EE91 pKa = 3.95 LEE93 pKa = 4.25 AMQQ96 pKa = 4.63
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.629
IPC2_protein 4.546
IPC_protein 4.393
Toseland 4.24
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.495
Wikipedia 4.202
Rodwell 4.228
Grimsley 4.151
Solomon 4.329
Lehninger 4.279
Nozaki 4.444
DTASelect 4.584
Thurlkill 4.24
EMBOSS 4.228
Sillero 4.495
Patrickios 4.088
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.423
Protein with the highest isoelectric point:
>tr|A0A4D5ZJK8|A0A4D5ZJK8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan328 OX=2558640 GN=JavanS328_0011 PE=4 SV=1
MM1 pKa = 7.84 KK2 pKa = 9.12 ITEE5 pKa = 4.39 VIKK8 pKa = 10.77 KK9 pKa = 10.02 DD10 pKa = 3.19 GSKK13 pKa = 10.33 VYY15 pKa = 10.14 RR16 pKa = 11.84 ANVYY20 pKa = 10.6 LGVDD24 pKa = 3.13 QVTGKK29 pKa = 10.2 KK30 pKa = 10.32 VKK32 pKa = 9.81 TKK34 pKa = 9.67 VTGRR38 pKa = 11.84 TQKK41 pKa = 10.03 EE42 pKa = 4.46 VKK44 pKa = 10.09 QKK46 pKa = 9.49 ATQEE50 pKa = 4.02 KK51 pKa = 10.11 IAFQKK56 pKa = 10.81 AGSTRR61 pKa = 11.84 QKK63 pKa = 11.11 ASTIKK68 pKa = 10.57 NYY70 pKa = 10.28 QEE72 pKa = 4.69 LANLWLEE79 pKa = 4.39 SYY81 pKa = 11.14 KK82 pKa = 10.15 NTVKK86 pKa = 10.71 PNTQGNVRR94 pKa = 11.84 KK95 pKa = 10.09 LIDD98 pKa = 3.46 NHH100 pKa = 6.27 ILPIFGAYY108 pKa = 9.61 KK109 pKa = 10.19 LDD111 pKa = 3.67 KK112 pKa = 10.12 LTTPLIQSIINEE124 pKa = 4.36 LADD127 pKa = 3.28 KK128 pKa = 10.13 TNRR131 pKa = 11.84 GEE133 pKa = 4.1 KK134 pKa = 10.44 GAFLHH139 pKa = 6.19 YY140 pKa = 10.82 DD141 pKa = 4.02 KK142 pKa = 11.05 IHH144 pKa = 6.59 ALNKK148 pKa = 10.18 RR149 pKa = 11.84 ILQYY153 pKa = 10.89 GVTMQAIPSNPARR166 pKa = 11.84 DD167 pKa = 3.48 VVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.19 AKK178 pKa = 8.99 RR179 pKa = 11.84 QKK181 pKa = 10.16 VKK183 pKa = 10.5 HH184 pKa = 5.86 FEE186 pKa = 3.84 NQEE189 pKa = 3.57 IKK191 pKa = 10.82 KK192 pKa = 10.24 FLGYY196 pKa = 10.47 LDD198 pKa = 5.12 NLDD201 pKa = 3.56 SDD203 pKa = 4.32 RR204 pKa = 11.84 YY205 pKa = 8.96 RR206 pKa = 11.84 YY207 pKa = 10.17 YY208 pKa = 11.31 YY209 pKa = 8.28 EE210 pKa = 3.62 TTLYY214 pKa = 10.88 KK215 pKa = 10.46 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.88 IDD236 pKa = 4.45 LDD238 pKa = 3.82 NAVVHH243 pKa = 5.2 VTKK246 pKa = 10.18 TLNRR250 pKa = 11.84 DD251 pKa = 3.49 LEE253 pKa = 4.53 INSPKK258 pKa = 10.62 SKK260 pKa = 10.68 ASYY263 pKa = 10.37 RR264 pKa = 11.84 DD265 pKa = 2.95 IDD267 pKa = 3.41 IDD269 pKa = 3.65 QATVSMLKK277 pKa = 9.91 QYY279 pKa = 11.27 KK280 pKa = 9.69 LRR282 pKa = 11.84 QTKK285 pKa = 7.97 EE286 pKa = 3.19 AWKK289 pKa = 9.48 IGQRR293 pKa = 11.84 EE294 pKa = 4.21 SVVFSDD300 pKa = 6.46 FIHH303 pKa = 7.06 EE304 pKa = 4.42 YY305 pKa = 9.91 PSSSRR310 pKa = 11.84 LKK312 pKa = 10.58 RR313 pKa = 11.84 RR314 pKa = 11.84 LQTHH318 pKa = 6.76 FKK320 pKa = 10.46 RR321 pKa = 11.84 ADD323 pKa = 3.23 VPNIGFHH330 pKa = 6.1 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.03 KK348 pKa = 10.3 EE349 pKa = 3.58 LQYY352 pKa = 11.41 RR353 pKa = 11.84 LGHH356 pKa = 5.67 STLSMTMDD364 pKa = 3.58 IYY366 pKa = 11.68 SHH368 pKa = 6.98 LSKK371 pKa = 11.0 EE372 pKa = 4.25 NAKK375 pKa = 10.37 KK376 pKa = 10.37 AVSFYY381 pKa = 9.42 EE382 pKa = 4.24 TALKK386 pKa = 10.67 ALL388 pKa = 4.15
Molecular weight: 44.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.066
IPC2_protein 9.326
IPC_protein 9.224
Toseland 10.028
ProMoST 9.663
Dawson 10.218
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.76
Grimsley 10.277
Solomon 10.233
Lehninger 10.189
Nozaki 9.999
DTASelect 9.853
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.116
Patrickios 10.321
IPC_peptide 10.233
IPC2_peptide 8.302
IPC2.peptide.svr19 8.295
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2569
51
488
151.1
17.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.566 ± 0.375
0.272 ± 0.087
6.306 ± 0.334
8.33 ± 0.648
3.581 ± 0.34
4.905 ± 0.368
1.985 ± 0.292
6.734 ± 0.543
9.031 ± 0.66
10.782 ± 0.684
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.383
5.917 ± 0.675
2.647 ± 0.372
4.009 ± 0.518
4.905 ± 0.402
6.033 ± 0.35
5.917 ± 0.45
5.372 ± 0.366
0.895 ± 0.159
4.438 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here