Massilia sp. ONC3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9T868|A0A4Y9T868_9BURK Uncharacterized protein OS=Massilia sp. ONC3 OX=2562153 GN=E4O92_01090 PE=4 SV=1
MM1 pKa = 7.15NAVAEE6 pKa = 4.24AQEE9 pKa = 4.75TIPSPIIFTDD19 pKa = 3.54SAAQKK24 pKa = 9.24VAQLIEE30 pKa = 4.37EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.58NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.48QYY84 pKa = 11.78LLGAEE89 pKa = 4.25IDD91 pKa = 4.0YY92 pKa = 11.26KK93 pKa = 11.41DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.56NPNAQSTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9SY34|A0A4Y9SY34_9BURK Cupin domain-containing protein OS=Massilia sp. ONC3 OX=2562153 GN=E4O92_14170 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84CPFKK6 pKa = 10.75LSRR9 pKa = 11.84AAPRR13 pKa = 11.84IRR15 pKa = 11.84AHH17 pKa = 6.9RR18 pKa = 11.84ARR20 pKa = 11.84ARR22 pKa = 11.84AAPRR26 pKa = 11.84ARR28 pKa = 11.84PAHH31 pKa = 6.48RR32 pKa = 11.84APPAPPAPARR42 pKa = 11.84ASSRR46 pKa = 11.84LSPLPPPLPRR56 pKa = 11.84TTRR59 pKa = 11.84RR60 pKa = 11.84PKK62 pKa = 8.44NTKK65 pKa = 9.63RR66 pKa = 11.84PPRR69 pKa = 11.84AAPAAPAAPAAPAVQPARR87 pKa = 11.84PAPAVARR94 pKa = 11.84AVLVAPAAPAAPAAPAAQRR113 pKa = 11.84GPAAA117 pKa = 4.35

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4650

0

4650

1599580

16

2145

344.0

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.213 ± 0.049

0.917 ± 0.012

5.325 ± 0.023

5.382 ± 0.03

3.628 ± 0.021

8.174 ± 0.033

2.215 ± 0.018

4.663 ± 0.026

3.58 ± 0.026

10.385 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.016

3.11 ± 0.023

5.193 ± 0.03

3.951 ± 0.021

6.729 ± 0.028

5.474 ± 0.027

5.204 ± 0.03

7.439 ± 0.031

1.372 ± 0.014

2.565 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski