Amino acid dipepetide frequency for Ralstonia phage Albius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.114AlaAla: 19.114 ± 1.563
1.082AlaCys: 1.082 ± 0.27
7.501AlaAsp: 7.501 ± 0.675
6.924AlaGlu: 6.924 ± 1.108
3.679AlaPhe: 3.679 ± 0.51
9.737AlaGly: 9.737 ± 1.063
1.587AlaHis: 1.587 ± 0.35
5.626AlaIle: 5.626 ± 0.527
5.842AlaLys: 5.842 ± 0.861
11.685AlaLeu: 11.685 ± 0.98
3.534AlaMet: 3.534 ± 0.495
5.049AlaAsn: 5.049 ± 0.638
4.039AlaPro: 4.039 ± 0.523
6.131AlaGln: 6.131 ± 0.713
5.626AlaArg: 5.626 ± 0.644
8.078AlaSer: 8.078 ± 0.714
7.141AlaThr: 7.141 ± 0.967
8.583AlaVal: 8.583 ± 1.0
1.803AlaTrp: 1.803 ± 0.368
3.534AlaTyr: 3.534 ± 0.534
0.0AlaXaa: 0.0 ± 0.0
Cys
1.01CysAla: 1.01 ± 0.231
0.433CysCys: 0.433 ± 0.196
0.433CysAsp: 0.433 ± 0.198
0.721CysGlu: 0.721 ± 0.217
0.072CysPhe: 0.072 ± 0.074
1.154CysGly: 1.154 ± 0.299
0.361CysHis: 0.361 ± 0.181
0.289CysIle: 0.289 ± 0.142
1.226CysLys: 1.226 ± 0.326
0.505CysLeu: 0.505 ± 0.184
0.144CysMet: 0.144 ± 0.096
0.721CysAsn: 0.721 ± 0.217
0.361CysPro: 0.361 ± 0.152
0.289CysGln: 0.289 ± 0.13
0.938CysArg: 0.938 ± 0.275
0.721CysSer: 0.721 ± 0.292
0.433CysThr: 0.433 ± 0.194
0.361CysVal: 0.361 ± 0.16
0.216CysTrp: 0.216 ± 0.132
0.577CysTyr: 0.577 ± 0.335
0.0CysXaa: 0.0 ± 0.0
Asp
7.285AspAla: 7.285 ± 0.766
0.721AspCys: 0.721 ± 0.259
3.895AspAsp: 3.895 ± 0.578
3.895AspGlu: 3.895 ± 0.66
2.164AspPhe: 2.164 ± 0.422
4.905AspGly: 4.905 ± 0.622
1.082AspHis: 1.082 ± 0.324
2.38AspIle: 2.38 ± 0.393
1.875AspLys: 1.875 ± 0.391
4.761AspLeu: 4.761 ± 0.605
1.154AspMet: 1.154 ± 0.247
1.659AspAsn: 1.659 ± 0.297
2.741AspPro: 2.741 ± 0.455
2.525AspGln: 2.525 ± 0.464
2.597AspArg: 2.597 ± 0.381
3.102AspSer: 3.102 ± 0.451
2.741AspThr: 2.741 ± 0.44
3.246AspVal: 3.246 ± 0.472
0.793AspTrp: 0.793 ± 0.236
1.515AspTyr: 1.515 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
5.77GluAla: 5.77 ± 0.898
0.361GluCys: 0.361 ± 0.152
1.587GluAsp: 1.587 ± 0.331
2.669GluGlu: 2.669 ± 0.523
1.226GluPhe: 1.226 ± 0.352
3.462GluGly: 3.462 ± 0.546
1.154GluHis: 1.154 ± 0.408
3.174GluIle: 3.174 ± 0.559
2.164GluLys: 2.164 ± 0.428
4.039GluLeu: 4.039 ± 0.614
1.515GluMet: 1.515 ± 0.271
1.515GluAsn: 1.515 ± 0.327
2.092GluPro: 2.092 ± 0.371
2.813GluGln: 2.813 ± 0.471
3.174GluArg: 3.174 ± 0.592
2.813GluSer: 2.813 ± 0.569
2.525GluThr: 2.525 ± 0.495
3.39GluVal: 3.39 ± 0.512
1.659GluTrp: 1.659 ± 0.356
2.308GluTyr: 2.308 ± 0.477
0.0GluXaa: 0.0 ± 0.0
Phe
3.895PheAla: 3.895 ± 0.708
0.433PheCys: 0.433 ± 0.187
2.164PheAsp: 2.164 ± 0.353
1.154PheGlu: 1.154 ± 0.343
0.938PhePhe: 0.938 ± 0.285
2.813PheGly: 2.813 ± 0.517
0.721PheHis: 0.721 ± 0.213
0.721PheIle: 0.721 ± 0.223
1.587PheLys: 1.587 ± 0.432
1.587PheLeu: 1.587 ± 0.335
0.649PheMet: 0.649 ± 0.227
1.082PheAsn: 1.082 ± 0.277
1.515PhePro: 1.515 ± 0.374
1.226PheGln: 1.226 ± 0.321
2.236PheArg: 2.236 ± 0.572
1.947PheSer: 1.947 ± 0.294
1.37PheThr: 1.37 ± 0.321
2.452PheVal: 2.452 ± 0.456
0.577PheTrp: 0.577 ± 0.2
1.01PheTyr: 1.01 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
9.377GlyAla: 9.377 ± 1.0
0.866GlyCys: 0.866 ± 0.253
3.462GlyAsp: 3.462 ± 0.579
3.174GlyGlu: 3.174 ± 0.588
2.525GlyPhe: 2.525 ± 0.51
8.078GlyGly: 8.078 ± 1.234
1.226GlyHis: 1.226 ± 0.291
4.472GlyIle: 4.472 ± 0.495
3.318GlyLys: 3.318 ± 0.525
6.708GlyLeu: 6.708 ± 0.629
2.885GlyMet: 2.885 ± 0.438
3.246GlyAsn: 3.246 ± 0.492
2.164GlyPro: 2.164 ± 0.454
3.606GlyGln: 3.606 ± 0.544
4.039GlyArg: 4.039 ± 0.71
4.833GlySer: 4.833 ± 0.818
7.213GlyThr: 7.213 ± 0.985
5.626GlyVal: 5.626 ± 0.607
1.587GlyTrp: 1.587 ± 0.357
3.246GlyTyr: 3.246 ± 0.652
0.0GlyXaa: 0.0 ± 0.0
His
1.875HisAla: 1.875 ± 0.414
0.289HisCys: 0.289 ± 0.16
0.866HisAsp: 0.866 ± 0.237
0.938HisGlu: 0.938 ± 0.336
0.649HisPhe: 0.649 ± 0.202
1.37HisGly: 1.37 ± 0.307
0.721HisHis: 0.721 ± 0.269
1.154HisIle: 1.154 ± 0.3
0.866HisLys: 0.866 ± 0.28
1.01HisLeu: 1.01 ± 0.273
0.361HisMet: 0.361 ± 0.186
0.938HisAsn: 0.938 ± 0.247
1.082HisPro: 1.082 ± 0.281
0.577HisGln: 0.577 ± 0.201
1.154HisArg: 1.154 ± 0.29
1.37HisSer: 1.37 ± 0.304
0.938HisThr: 0.938 ± 0.3
1.01HisVal: 1.01 ± 0.287
0.216HisTrp: 0.216 ± 0.145
0.505HisTyr: 0.505 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
5.049IleAla: 5.049 ± 0.506
0.577IleCys: 0.577 ± 0.261
4.256IleAsp: 4.256 ± 0.579
2.813IleGlu: 2.813 ± 0.388
1.01IlePhe: 1.01 ± 0.197
3.895IleGly: 3.895 ± 0.578
0.721IleHis: 0.721 ± 0.241
2.092IleIle: 2.092 ± 0.384
2.38IleLys: 2.38 ± 0.438
2.813IleLeu: 2.813 ± 0.491
0.866IleMet: 0.866 ± 0.232
2.164IleAsn: 2.164 ± 0.512
1.875IlePro: 1.875 ± 0.417
2.525IleGln: 2.525 ± 0.406
2.092IleArg: 2.092 ± 0.372
2.525IleSer: 2.525 ± 0.469
3.174IleThr: 3.174 ± 0.603
4.4IleVal: 4.4 ± 0.559
0.361IleTrp: 0.361 ± 0.193
1.947IleTyr: 1.947 ± 0.373
0.0IleXaa: 0.0 ± 0.0
Lys
4.977LysAla: 4.977 ± 0.825
0.361LysCys: 0.361 ± 0.174
2.092LysAsp: 2.092 ± 0.458
2.02LysGlu: 2.02 ± 0.521
1.082LysPhe: 1.082 ± 0.263
2.813LysGly: 2.813 ± 0.548
1.01LysHis: 1.01 ± 0.249
2.164LysIle: 2.164 ± 0.551
1.659LysLys: 1.659 ± 0.404
4.111LysLeu: 4.111 ± 0.583
0.866LysMet: 0.866 ± 0.329
1.515LysAsn: 1.515 ± 0.32
2.452LysPro: 2.452 ± 0.484
2.38LysGln: 2.38 ± 0.398
3.246LysArg: 3.246 ± 0.587
2.525LysSer: 2.525 ± 0.358
2.957LysThr: 2.957 ± 0.508
2.308LysVal: 2.308 ± 0.363
0.577LysTrp: 0.577 ± 0.22
1.082LysTyr: 1.082 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
10.747LeuAla: 10.747 ± 1.297
0.793LeuCys: 0.793 ± 0.23
5.121LeuAsp: 5.121 ± 0.516
3.679LeuGlu: 3.679 ± 0.45
2.38LeuPhe: 2.38 ± 0.384
5.554LeuGly: 5.554 ± 0.652
1.947LeuHis: 1.947 ± 0.365
3.751LeuIle: 3.751 ± 0.488
3.029LeuLys: 3.029 ± 0.484
5.41LeuLeu: 5.41 ± 0.795
2.02LeuMet: 2.02 ± 0.437
3.39LeuAsn: 3.39 ± 0.399
3.679LeuPro: 3.679 ± 0.52
4.4LeuGln: 4.4 ± 0.479
6.059LeuArg: 6.059 ± 0.81
4.183LeuSer: 4.183 ± 0.565
5.626LeuThr: 5.626 ± 0.758
4.688LeuVal: 4.688 ± 0.485
0.721LeuTrp: 0.721 ± 0.214
1.587LeuTyr: 1.587 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
3.39MetAla: 3.39 ± 0.498
0.0MetCys: 0.0 ± 0.0
0.793MetAsp: 0.793 ± 0.273
1.443MetGlu: 1.443 ± 0.328
0.793MetPhe: 0.793 ± 0.218
1.803MetGly: 1.803 ± 0.36
0.361MetHis: 0.361 ± 0.159
1.01MetIle: 1.01 ± 0.289
1.226MetLys: 1.226 ± 0.317
2.092MetLeu: 2.092 ± 0.442
0.577MetMet: 0.577 ± 0.238
1.298MetAsn: 1.298 ± 0.414
1.01MetPro: 1.01 ± 0.229
1.01MetGln: 1.01 ± 0.261
1.659MetArg: 1.659 ± 0.401
2.02MetSer: 2.02 ± 0.336
2.452MetThr: 2.452 ± 0.401
1.515MetVal: 1.515 ± 0.387
0.072MetTrp: 0.072 ± 0.067
0.649MetTyr: 0.649 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
5.987AsnAla: 5.987 ± 0.798
0.289AsnCys: 0.289 ± 0.138
1.298AsnAsp: 1.298 ± 0.249
1.01AsnGlu: 1.01 ± 0.272
1.37AsnPhe: 1.37 ± 0.31
4.977AsnGly: 4.977 ± 0.652
0.361AsnHis: 0.361 ± 0.17
1.443AsnIle: 1.443 ± 0.323
1.01AsnLys: 1.01 ± 0.253
2.669AsnLeu: 2.669 ± 0.411
0.866AsnMet: 0.866 ± 0.277
1.226AsnAsn: 1.226 ± 0.338
3.174AsnPro: 3.174 ± 0.725
1.803AsnGln: 1.803 ± 0.38
1.947AsnArg: 1.947 ± 0.346
2.741AsnSer: 2.741 ± 0.501
2.02AsnThr: 2.02 ± 0.558
3.029AsnVal: 3.029 ± 0.489
1.082AsnTrp: 1.082 ± 0.28
1.298AsnTyr: 1.298 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
4.833ProAla: 4.833 ± 0.748
0.289ProCys: 0.289 ± 0.127
2.741ProAsp: 2.741 ± 0.487
2.813ProGlu: 2.813 ± 0.581
1.154ProPhe: 1.154 ± 0.315
2.957ProGly: 2.957 ± 0.502
0.793ProHis: 0.793 ± 0.271
2.236ProIle: 2.236 ± 0.442
1.298ProLys: 1.298 ± 0.276
2.597ProLeu: 2.597 ± 0.405
1.154ProMet: 1.154 ± 0.272
2.452ProAsn: 2.452 ± 0.642
2.236ProPro: 2.236 ± 0.466
1.947ProGln: 1.947 ± 0.435
2.308ProArg: 2.308 ± 0.449
3.246ProSer: 3.246 ± 0.44
4.183ProThr: 4.183 ± 0.617
3.823ProVal: 3.823 ± 0.553
0.361ProTrp: 0.361 ± 0.167
1.154ProTyr: 1.154 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
7.285GlnAla: 7.285 ± 0.701
0.721GlnCys: 0.721 ± 0.163
2.092GlnAsp: 2.092 ± 0.348
2.308GlnGlu: 2.308 ± 0.402
1.587GlnPhe: 1.587 ± 0.266
3.823GlnGly: 3.823 ± 0.575
0.938GlnHis: 0.938 ± 0.237
2.38GlnIle: 2.38 ± 0.549
1.947GlnLys: 1.947 ± 0.358
3.462GlnLeu: 3.462 ± 0.701
1.37GlnMet: 1.37 ± 0.24
1.659GlnAsn: 1.659 ± 0.324
2.308GlnPro: 2.308 ± 0.448
3.174GlnGln: 3.174 ± 0.517
2.669GlnArg: 2.669 ± 0.423
3.318GlnSer: 3.318 ± 0.452
3.102GlnThr: 3.102 ± 0.575
3.246GlnVal: 3.246 ± 0.558
0.505GlnTrp: 0.505 ± 0.192
0.866GlnTyr: 0.866 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
6.492ArgAla: 6.492 ± 0.8
0.866ArgCys: 0.866 ± 0.281
2.957ArgAsp: 2.957 ± 0.468
4.256ArgGlu: 4.256 ± 0.622
2.02ArgPhe: 2.02 ± 0.382
3.606ArgGly: 3.606 ± 0.482
1.01ArgHis: 1.01 ± 0.268
2.957ArgIle: 2.957 ± 0.577
3.534ArgLys: 3.534 ± 0.624
5.338ArgLeu: 5.338 ± 0.624
1.731ArgMet: 1.731 ± 0.503
1.731ArgAsn: 1.731 ± 0.337
2.02ArgPro: 2.02 ± 0.373
2.525ArgGln: 2.525 ± 0.439
3.534ArgArg: 3.534 ± 0.612
3.029ArgSer: 3.029 ± 0.548
2.669ArgThr: 2.669 ± 0.459
3.823ArgVal: 3.823 ± 0.563
1.37ArgTrp: 1.37 ± 0.293
1.154ArgTyr: 1.154 ± 0.265
0.0ArgXaa: 0.0 ± 0.0
Ser
7.79SerAla: 7.79 ± 0.923
0.505SerCys: 0.505 ± 0.189
3.246SerAsp: 3.246 ± 0.533
2.164SerGlu: 2.164 ± 0.378
2.02SerPhe: 2.02 ± 0.287
6.636SerGly: 6.636 ± 0.886
0.721SerHis: 0.721 ± 0.21
4.039SerIle: 4.039 ± 0.548
2.885SerLys: 2.885 ± 0.563
4.761SerLeu: 4.761 ± 0.571
1.443SerMet: 1.443 ± 0.332
2.525SerAsn: 2.525 ± 0.482
2.957SerPro: 2.957 ± 0.392
2.885SerGln: 2.885 ± 0.428
2.597SerArg: 2.597 ± 0.496
4.328SerSer: 4.328 ± 0.69
4.472SerThr: 4.472 ± 0.642
4.472SerVal: 4.472 ± 0.583
0.938SerTrp: 0.938 ± 0.236
1.875SerTyr: 1.875 ± 0.455
0.0SerXaa: 0.0 ± 0.0
Thr
7.862ThrAla: 7.862 ± 0.984
0.433ThrCys: 0.433 ± 0.147
3.534ThrAsp: 3.534 ± 0.434
2.164ThrGlu: 2.164 ± 0.441
2.236ThrPhe: 2.236 ± 0.458
5.698ThrGly: 5.698 ± 0.857
1.082ThrHis: 1.082 ± 0.316
2.669ThrIle: 2.669 ± 0.451
2.02ThrLys: 2.02 ± 0.345
6.924ThrLeu: 6.924 ± 0.76
1.082ThrMet: 1.082 ± 0.272
2.741ThrAsn: 2.741 ± 0.602
3.606ThrPro: 3.606 ± 0.576
2.885ThrGln: 2.885 ± 0.421
3.39ThrArg: 3.39 ± 0.468
4.183ThrSer: 4.183 ± 0.579
4.039ThrThr: 4.039 ± 0.963
5.842ThrVal: 5.842 ± 0.835
1.01ThrTrp: 1.01 ± 0.263
2.02ThrTyr: 2.02 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
9.088ValAla: 9.088 ± 0.987
1.01ValCys: 1.01 ± 0.263
4.4ValAsp: 4.4 ± 0.759
3.679ValGlu: 3.679 ± 0.557
1.731ValPhe: 1.731 ± 0.338
5.338ValGly: 5.338 ± 0.668
1.154ValHis: 1.154 ± 0.335
2.957ValIle: 2.957 ± 0.469
2.452ValLys: 2.452 ± 0.606
4.616ValLeu: 4.616 ± 0.591
1.731ValMet: 1.731 ± 0.419
3.174ValAsn: 3.174 ± 0.58
3.246ValPro: 3.246 ± 0.585
3.679ValGln: 3.679 ± 0.538
3.462ValArg: 3.462 ± 0.556
5.049ValSer: 5.049 ± 0.734
6.275ValThr: 6.275 ± 0.776
5.121ValVal: 5.121 ± 1.051
1.37ValTrp: 1.37 ± 0.261
1.659ValTyr: 1.659 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
1.443TrpAla: 1.443 ± 0.387
0.289TrpCys: 0.289 ± 0.123
0.577TrpAsp: 0.577 ± 0.187
0.577TrpGlu: 0.577 ± 0.171
0.721TrpPhe: 0.721 ± 0.225
0.505TrpGly: 0.505 ± 0.167
0.505TrpHis: 0.505 ± 0.183
0.649TrpIle: 0.649 ± 0.218
0.649TrpLys: 0.649 ± 0.214
1.659TrpLeu: 1.659 ± 0.329
0.361TrpMet: 0.361 ± 0.184
0.721TrpAsn: 0.721 ± 0.217
1.082TrpPro: 1.082 ± 0.285
0.793TrpGln: 0.793 ± 0.244
1.01TrpArg: 1.01 ± 0.306
1.226TrpSer: 1.226 ± 0.29
0.721TrpThr: 0.721 ± 0.211
1.443TrpVal: 1.443 ± 0.267
0.0TrpTrp: 0.0 ± 0.0
0.649TrpTyr: 0.649 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.029TyrAla: 3.029 ± 0.524
0.649TyrCys: 0.649 ± 0.193
2.02TyrAsp: 2.02 ± 0.388
1.01TyrGlu: 1.01 ± 0.348
0.721TyrPhe: 0.721 ± 0.201
2.525TyrGly: 2.525 ± 0.422
0.433TyrHis: 0.433 ± 0.194
1.37TyrIle: 1.37 ± 0.288
1.226TyrLys: 1.226 ± 0.321
2.02TyrLeu: 2.02 ± 0.378
0.793TyrMet: 0.793 ± 0.218
0.793TyrAsn: 0.793 ± 0.272
0.938TyrPro: 0.938 ± 0.217
1.587TyrGln: 1.587 ± 0.314
2.885TyrArg: 2.885 ± 0.462
2.02TyrSer: 2.02 ± 0.395
1.37TyrThr: 1.37 ± 0.302
2.813TyrVal: 2.813 ± 0.417
0.433TyrTrp: 0.433 ± 0.17
1.37TyrTyr: 1.37 ± 0.334
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (13865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski