Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus delbrueckii; Lactobacillus delbrueckii subsp. bulgaricus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1GB80|Q1GB80_LACDA Conserved hypothetical membrane protein OS=Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) OX=390333 GN=Ldb0564 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.45FNKK5 pKa = 8.41QTPIIVRR12 pKa = 11.84AFHH15 pKa = 6.96YY16 pKa = 10.65DD17 pKa = 3.36LLDD20 pKa = 3.84EE21 pKa = 4.8PEE23 pKa = 4.54EE24 pKa = 4.26KK25 pKa = 11.0NEE27 pKa = 3.72VNVAIRR33 pKa = 11.84QVVATGEE40 pKa = 4.3DD41 pKa = 3.77GVEE44 pKa = 4.15DD45 pKa = 3.73AGEE48 pKa = 3.96AGGYY52 pKa = 9.25YY53 pKa = 9.71EE54 pKa = 4.21VAVVYY59 pKa = 10.47DD60 pKa = 3.69VTPDD64 pKa = 3.25EE65 pKa = 5.02DD66 pKa = 4.24NIIEE70 pKa = 3.98ISGVNTQVVQLLGYY84 pKa = 10.16HH85 pKa = 7.22GDD87 pKa = 3.91GQDD90 pKa = 4.66LDD92 pKa = 3.82QEE94 pKa = 4.87TYY96 pKa = 10.91RR97 pKa = 11.84LLSRR101 pKa = 11.84PLVEE105 pKa = 4.55YY106 pKa = 10.51IEE108 pKa = 4.3TLTYY112 pKa = 10.28EE113 pKa = 4.42VSQVALDD120 pKa = 3.79EE121 pKa = 5.17PINLDD126 pKa = 3.74FEE128 pKa = 5.61PNFF131 pKa = 3.8

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1G9E6|Q1G9E6_LACDA Cysteine synthase OS=Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) OX=390333 GN=cysK1 PE=3 SV=1
MM1 pKa = 7.87PKK3 pKa = 9.59MKK5 pKa = 8.8THH7 pKa = 6.78RR8 pKa = 11.84ASAKK12 pKa = 9.12RR13 pKa = 11.84FKK15 pKa = 10.26RR16 pKa = 11.84TGNGGLKK23 pKa = 9.42RR24 pKa = 11.84HH25 pKa = 6.17HH26 pKa = 7.23AFTGHH31 pKa = 6.77RR32 pKa = 11.84FHH34 pKa = 7.54GKK36 pKa = 6.24TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.45LRR45 pKa = 11.84KK46 pKa = 8.75AAMVSRR52 pKa = 11.84SDD54 pKa = 3.67LKK56 pKa = 10.76RR57 pKa = 11.84IKK59 pKa = 10.66QMLSQMRR66 pKa = 3.59

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1519

0

1519

450174

16

1946

296.4

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.64 ± 0.066

0.666 ± 0.017

5.941 ± 0.063

6.154 ± 0.072

4.186 ± 0.053

6.807 ± 0.053

1.824 ± 0.026

6.269 ± 0.056

7.283 ± 0.067

10.245 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.03

4.161 ± 0.037

3.519 ± 0.034

4.236 ± 0.052

4.354 ± 0.048

6.201 ± 0.071

5.187 ± 0.052

6.976 ± 0.049

0.999 ± 0.022

3.782 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski