Ruminococcus albus 8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; Ruminococcus albus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9S7F5|E9S7F5_RUMAL Putative lipoprotein OS=Ruminococcus albus 8 OX=246199 GN=CUS_6166 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.44KK3 pKa = 10.44LIAVICAVALCCGGLAGCGDD23 pKa = 4.12KK24 pKa = 10.84KK25 pKa = 10.83SEE27 pKa = 4.33KK28 pKa = 10.29ADD30 pKa = 3.4STAEE34 pKa = 4.36KK35 pKa = 8.7ITSSAADD42 pKa = 3.34EE43 pKa = 4.5SVTNAEE49 pKa = 4.29SEE51 pKa = 4.35TTEE54 pKa = 4.18TEE56 pKa = 4.23EE57 pKa = 3.8LTMAEE62 pKa = 5.0AYY64 pKa = 8.01TKK66 pKa = 10.56SIEE69 pKa = 4.18NANFRR74 pKa = 11.84TLMVTSSDD82 pKa = 3.66FTDD85 pKa = 3.76DD86 pKa = 3.05TTTMVEE92 pKa = 4.02VSGDD96 pKa = 3.9DD97 pKa = 3.4YY98 pKa = 11.77HH99 pKa = 7.27MSVGDD104 pKa = 4.05GEE106 pKa = 4.72NQTEE110 pKa = 4.64LYY112 pKa = 10.58VIGGVMYY119 pKa = 9.73MLSHH123 pKa = 7.06AEE125 pKa = 3.43KK126 pKa = 10.57SYY128 pKa = 11.26IKK130 pKa = 10.65DD131 pKa = 3.4EE132 pKa = 4.76TPDD135 pKa = 3.41EE136 pKa = 4.59MYY138 pKa = 11.45LNMDD142 pKa = 3.99TSNYY146 pKa = 8.71TMGVDD151 pKa = 3.9DD152 pKa = 5.57SYY154 pKa = 11.98IFIDD158 pKa = 3.76SVIAEE163 pKa = 4.41GDD165 pKa = 4.27LICEE169 pKa = 4.55TYY171 pKa = 10.25HH172 pKa = 8.07APDD175 pKa = 5.49LISGEE180 pKa = 4.23MPSGDD185 pKa = 3.5EE186 pKa = 4.23DD187 pKa = 5.3GEE189 pKa = 4.65VTVYY193 pKa = 10.48KK194 pKa = 10.79YY195 pKa = 11.12YY196 pKa = 10.68FEE198 pKa = 6.11KK199 pKa = 10.77DD200 pKa = 2.55GKK202 pKa = 8.36TPVKK206 pKa = 10.12IEE208 pKa = 3.6MSAYY212 pKa = 10.07GLEE215 pKa = 4.0QTTTFKK221 pKa = 10.87EE222 pKa = 4.35FSFDD226 pKa = 2.84IDD228 pKa = 4.31SIVIPDD234 pKa = 4.01LSDD237 pKa = 3.04WTDD240 pKa = 3.38DD241 pKa = 3.9TEE243 pKa = 4.91SSGFGLDD250 pKa = 3.04TDD252 pKa = 4.27VEE254 pKa = 4.4SEE256 pKa = 4.27EE257 pKa = 4.51LDD259 pKa = 3.51EE260 pKa = 5.8SEE262 pKa = 4.55FDD264 pKa = 4.18ADD266 pKa = 3.89IDD268 pKa = 4.49VEE270 pKa = 4.22KK271 pKa = 11.02ADD273 pKa = 4.03AAEE276 pKa = 4.36TEE278 pKa = 4.27

Molecular weight: 30.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9SDG0|E9SDG0_RUMAL Diguanylate cyclase (GGDEF) domain protein OS=Ruminococcus albus 8 OX=246199 GN=CUS_7146 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.19RR3 pKa = 11.84PNFMQGRR10 pKa = 11.84YY11 pKa = 10.25GFDD14 pKa = 2.85TLFFANTALCLLIALLGYY32 pKa = 9.76IVLWRR37 pKa = 11.84IPHH40 pKa = 5.7VDD42 pKa = 3.08AHH44 pKa = 6.29SVIALSGLYY53 pKa = 9.44MIINISRR60 pKa = 11.84VFSKK64 pKa = 10.85DD65 pKa = 2.33IGKK68 pKa = 9.68RR69 pKa = 11.84EE70 pKa = 3.92NEE72 pKa = 3.82NLRR75 pKa = 11.84FTMRR79 pKa = 11.84MNSLFRR85 pKa = 11.84HH86 pKa = 5.77SKK88 pKa = 8.49PANSFDD94 pKa = 3.35PRR96 pKa = 11.84SAVRR100 pKa = 11.84PVRR103 pKa = 11.84GAHH106 pKa = 5.2TASARR111 pKa = 11.84CSCGAKK117 pKa = 10.32LPTPEE122 pKa = 3.82GSGRR126 pKa = 11.84QIVVCPVCGSRR137 pKa = 11.84NMVKK141 pKa = 10.38KK142 pKa = 10.72

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3826

0

3826

1141692

34

3065

298.4

33.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.048

1.723 ± 0.019

6.548 ± 0.033

7.222 ± 0.044

4.263 ± 0.028

7.079 ± 0.039

1.649 ± 0.017

6.929 ± 0.04

6.848 ± 0.037

8.247 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.021

4.507 ± 0.037

3.228 ± 0.021

2.581 ± 0.023

4.423 ± 0.04

6.267 ± 0.036

5.688 ± 0.045

6.797 ± 0.035

0.893 ± 0.017

4.203 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski