Torque teno virus (isolate Human/Japan/SANBAN/1999) (TTV)
Average proteome isoelectric point is 8.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9WSV8|ORF23_TTVV5 Probable protein VP2 OS=Torque teno virus (isolate Human/Japan/SANBAN/1999) OX=486277 GN=ORF2/3 PE=3 SV=2
MM1 pKa = 7.44 AWGWWRR7 pKa = 11.84 RR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 WPARR15 pKa = 11.84 RR16 pKa = 11.84 WRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 PLRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGRR33 pKa = 11.84 PARR36 pKa = 11.84 RR37 pKa = 11.84 YY38 pKa = 8.56 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 TVRR45 pKa = 11.84 TRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 WGRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 YY56 pKa = 8.99 RR57 pKa = 11.84 RR58 pKa = 11.84 GWRR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 TYY65 pKa = 9.77 VRR67 pKa = 11.84 KK68 pKa = 9.82 GRR70 pKa = 11.84 HH71 pKa = 4.43 RR72 pKa = 11.84 KK73 pKa = 8.58 KK74 pKa = 10.35 KK75 pKa = 9.62 KK76 pKa = 10.13 RR77 pKa = 11.84 LILRR81 pKa = 11.84 QWQPATRR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 CTITGYY96 pKa = 10.9 LPIVFCGHH104 pKa = 5.39 TKK106 pKa = 10.38 GNKK109 pKa = 9.43 NYY111 pKa = 10.35 ALHH114 pKa = 6.91 SDD116 pKa = 4.09 DD117 pKa = 4.72 YY118 pKa = 10.39 TPQGQPFGGALSTTSFSLKK137 pKa = 10.54 VLFDD141 pKa = 2.86 QHH143 pKa = 6.7 QRR145 pKa = 11.84 GLNKK149 pKa = 9.43 WSFPNDD155 pKa = 3.33 QLDD158 pKa = 3.49 LARR161 pKa = 11.84 YY162 pKa = 8.61 RR163 pKa = 11.84 GCKK166 pKa = 9.83 FIFYY170 pKa = 7.93 RR171 pKa = 11.84 TKK173 pKa = 8.03 QTDD176 pKa = 3.23 WIGQYY181 pKa = 10.53 DD182 pKa = 3.21 ISEE185 pKa = 4.46 PYY187 pKa = 10.63 KK188 pKa = 10.32 LDD190 pKa = 3.81 KK191 pKa = 11.02 YY192 pKa = 10.83 SCPNYY197 pKa = 10.43 HH198 pKa = 7.26 PGNLIKK204 pKa = 10.6 AKK206 pKa = 10.17 HH207 pKa = 5.88 KK208 pKa = 10.45 FLIPSYY214 pKa = 9.03 DD215 pKa = 3.43 TNPRR219 pKa = 11.84 GRR221 pKa = 11.84 QKK223 pKa = 10.5 IIVKK227 pKa = 10.06 IPPPDD232 pKa = 3.44 LFVDD236 pKa = 3.59 KK237 pKa = 10.35 WYY239 pKa = 8.82 TQEE242 pKa = 4.91 DD243 pKa = 4.53 LCSVNLVSLAVSAASFLHH261 pKa = 6.42 PFGSPQTDD269 pKa = 3.38 NPCYY273 pKa = 9.34 TFQVLKK279 pKa = 10.39 EE280 pKa = 4.24 FYY282 pKa = 8.67 YY283 pKa = 10.4 QAIGFSATDD292 pKa = 3.17 EE293 pKa = 4.13 SRR295 pKa = 11.84 NYY297 pKa = 9.48 VFNVLYY303 pKa = 10.44 EE304 pKa = 4.18 EE305 pKa = 4.04 NSYY308 pKa = 9.95 WEE310 pKa = 4.4 SNITPFYY317 pKa = 10.0 VINVKK322 pKa = 10.39 KK323 pKa = 10.83 GSNTXDD329 pKa = 3.46 YY330 pKa = 7.7 MSPXISDD337 pKa = 3.07 SHH339 pKa = 6.55 FRR341 pKa = 11.84 NKK343 pKa = 9.96 VNTNYY348 pKa = 10.32 NWYY351 pKa = 8.09 TYY353 pKa = 8.8 NAKK356 pKa = 9.05 SHH358 pKa = 6.75 KK359 pKa = 10.18 NDD361 pKa = 3.0 LHH363 pKa = 5.16 XLRR366 pKa = 11.84 RR367 pKa = 11.84 AYY369 pKa = 9.99 FKK371 pKa = 10.84 QLTTEE376 pKa = 5.26 GPQQTSSEE384 pKa = 4.13 KK385 pKa = 10.72 GYY387 pKa = 10.77 ASQWTTPTTDD397 pKa = 2.49 AYY399 pKa = 9.96 EE400 pKa = 4.0 YY401 pKa = 9.93 HH402 pKa = 7.78 LGMFSTIFLAPDD414 pKa = 3.23 RR415 pKa = 11.84 PVPRR419 pKa = 11.84 FPCAYY424 pKa = 10.13 QDD426 pKa = 3.4 VTYY429 pKa = 10.93 NPLMDD434 pKa = 4.37 KK435 pKa = 11.08 GVGNHH440 pKa = 5.86 VWFQYY445 pKa = 7.57 NTKK448 pKa = 10.57 ADD450 pKa = 3.56 TQLIVTGGSCKK461 pKa = 10.55 AHH463 pKa = 7.31 IEE465 pKa = 5.03 DD466 pKa = 3.86 IPLWAAFYY474 pKa = 10.09 GYY476 pKa = 10.81 SDD478 pKa = 4.68 FIEE481 pKa = 4.89 SEE483 pKa = 4.01 LGPFVDD489 pKa = 4.9 AEE491 pKa = 4.69 TVGLICVICPYY502 pKa = 9.55 TKK504 pKa = 10.07 PPMYY508 pKa = 10.75 NKK510 pKa = 8.53 TNPMMGYY517 pKa = 9.93 VFYY520 pKa = 10.94 DD521 pKa = 3.3 RR522 pKa = 11.84 NFGDD526 pKa = 4.44 GKK528 pKa = 10.1 WIDD531 pKa = 3.65 GRR533 pKa = 11.84 GKK535 pKa = 9.76 IEE537 pKa = 4.95 PYY539 pKa = 8.88 WQVRR543 pKa = 11.84 WRR545 pKa = 11.84 PEE547 pKa = 3.47 MLFQEE552 pKa = 4.6 TVMADD557 pKa = 3.2 IVQTGPFSYY566 pKa = 10.56 KK567 pKa = 10.63 DD568 pKa = 3.45 EE569 pKa = 4.92 LKK571 pKa = 11.1 NSTLVAKK578 pKa = 10.44 YY579 pKa = 10.31 KK580 pKa = 10.65 FYY582 pKa = 9.95 FTWGGNMMFQQTIKK596 pKa = 10.75 NPCKK600 pKa = 9.64 TDD602 pKa = 3.15 GRR604 pKa = 11.84 PTDD607 pKa = 3.43 SDD609 pKa = 3.35 RR610 pKa = 11.84 HH611 pKa = 5.0 PRR613 pKa = 11.84 GIQVADD619 pKa = 3.96 PEE621 pKa = 4.54 QMGPRR626 pKa = 11.84 WVFHH630 pKa = 5.99 SFDD633 pKa = 2.91 WRR635 pKa = 11.84 RR636 pKa = 11.84 GYY638 pKa = 10.88 LSEE641 pKa = 4.14 GAIKK645 pKa = 10.39 RR646 pKa = 11.84 LHH648 pKa = 6.42 EE649 pKa = 4.53 KK650 pKa = 10.18 PLDD653 pKa = 3.56 YY654 pKa = 10.75 EE655 pKa = 5.01 SYY657 pKa = 8.49 FTQPKK662 pKa = 9.16 RR663 pKa = 11.84 PRR665 pKa = 11.84 IFPPTEE671 pKa = 3.54 AAEE674 pKa = 4.62 GEE676 pKa = 4.1 FRR678 pKa = 11.84 EE679 pKa = 4.5 PEE681 pKa = 3.87 KK682 pKa = 10.94 GSYY685 pKa = 9.93 SEE687 pKa = 4.54 EE688 pKa = 3.9 EE689 pKa = 3.94 RR690 pKa = 11.84 SQASAEE696 pKa = 4.09 EE697 pKa = 4.14 QATEE701 pKa = 4.07 EE702 pKa = 4.21 TVLLLKK708 pKa = 10.53 RR709 pKa = 11.84 RR710 pKa = 11.84 LRR712 pKa = 11.84 EE713 pKa = 3.8 QRR715 pKa = 11.84 KK716 pKa = 9.48 LEE718 pKa = 3.91 QQLQFLTRR726 pKa = 11.84 EE727 pKa = 4.07 MFKK730 pKa = 9.1 TQAGLHH736 pKa = 5.98 INPMLLSQRR745 pKa = 3.87
Molecular weight: 88.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.842
IPC2_protein 9.443
IPC_protein 9.633
Toseland 9.867
ProMoST 9.721
Dawson 10.16
Bjellqvist 9.897
Wikipedia 10.365
Rodwell 10.394
Grimsley 10.248
Solomon 10.175
Lehninger 10.116
Nozaki 9.911
DTASelect 9.882
Thurlkill 9.984
EMBOSS 10.306
Sillero 10.072
Patrickios 5.334
IPC_peptide 10.16
IPC2_peptide 8.726
IPC2.peptide.svr19 8.44
Protein with the highest isoelectric point:
>sp|Q9WSV8|ORF23_TTVV5 Probable protein VP2 OS=Torque teno virus (isolate Human/Japan/SANBAN/1999) OX=486277 GN=ORF2/3 PE=3 SV=2
MM1 pKa = 7.17 SWRR4 pKa = 11.84 PPVHH8 pKa = 6.97 DD9 pKa = 4.22 APGIEE14 pKa = 4.18 RR15 pKa = 11.84 NWYY18 pKa = 6.33 EE19 pKa = 3.73 ACFRR23 pKa = 11.84 AHH25 pKa = 7.17 AGSCGCGNFIAHH37 pKa = 6.83 INLLAGRR44 pKa = 11.84 YY45 pKa = 8.5 GFTGGPPPPGGPPPGTPQVRR65 pKa = 11.84 ASRR68 pKa = 11.84 NSPAAPQQPPALPWHH83 pKa = 6.77 GDD85 pKa = 3.2 GGEE88 pKa = 4.37 GGAAGPPGAGGDD100 pKa = 3.76 AAADD104 pKa = 3.26 AHH106 pKa = 7.23 LGDD109 pKa = 5.07 EE110 pKa = 4.49 EE111 pKa = 5.42 LADD114 pKa = 4.33 LLDD117 pKa = 5.27 AIEE120 pKa = 6.17 DD121 pKa = 3.9 DD122 pKa = 4.22 AQCSNRR128 pKa = 11.84 RR129 pKa = 11.84 SKK131 pKa = 9.6 TRR133 pKa = 11.84 ARR135 pKa = 11.84 RR136 pKa = 11.84 TDD138 pKa = 3.64 GPPTPIDD145 pKa = 3.35 TLEE148 pKa = 4.0 EE149 pKa = 4.41 YY150 pKa = 10.12 KK151 pKa = 10.33 WRR153 pKa = 11.84 TRR155 pKa = 11.84 NKK157 pKa = 8.83 WDD159 pKa = 3.55 PAGCSTPLTGEE170 pKa = 3.93 GAILARR176 pKa = 11.84 EE177 pKa = 4.27 LSNACTRR184 pKa = 11.84 NLSTMKK190 pKa = 10.63 AILHH194 pKa = 5.21 NQKK197 pKa = 10.5 DD198 pKa = 4.2 LEE200 pKa = 4.4 SFLQQRR206 pKa = 11.84 QQRR209 pKa = 11.84 EE210 pKa = 4.13 SSEE213 pKa = 4.41 SPKK216 pKa = 10.15 KK217 pKa = 10.79 AHH219 pKa = 5.95 IQRR222 pKa = 11.84 KK223 pKa = 9.1 KK224 pKa = 10.14 GRR226 pKa = 11.84 KK227 pKa = 8.14 PLQKK231 pKa = 9.3 SRR233 pKa = 11.84 RR234 pKa = 11.84 RR235 pKa = 11.84 RR236 pKa = 11.84 RR237 pKa = 11.84 QYY239 pKa = 11.01 SSSSDD244 pKa = 3.41 DD245 pKa = 3.87 SEE247 pKa = 5.16 SSGSSSSSSNSSPEE261 pKa = 3.73 KK262 pKa = 8.75 CSKK265 pKa = 10.13 RR266 pKa = 11.84 KK267 pKa = 9.46 RR268 pKa = 11.84 VSTT271 pKa = 3.96
Molecular weight: 29.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.92
IPC2_protein 8.595
IPC_protein 8.551
Toseland 9.282
ProMoST 9.092
Dawson 9.56
Bjellqvist 9.428
Wikipedia 9.663
Rodwell 9.809
Grimsley 9.531
Solomon 9.648
Lehninger 9.619
Nozaki 9.706
DTASelect 9.297
Thurlkill 9.472
EMBOSS 9.75
Sillero 9.633
Patrickios 5.677
IPC_peptide 9.648
IPC2_peptide 8.331
IPC2.peptide.svr19 7.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1016
271
745
508.0
58.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.102 ± 1.99
1.772 ± 0.418
5.02 ± 0.266
5.118 ± 0.215
4.232 ± 1.421
7.087 ± 1.483
2.362 ± 0.114
3.74 ± 0.406
6.004 ± 0.432
6.201 ± 0.153
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.575 ± 0.431
4.035 ± 0.178
7.382 ± 1.14
4.823 ± 0.204
10.236 ± 0.331
6.988 ± 2.294
6.004 ± 0.812
3.346 ± 1.154
2.756 ± 0.47
4.921 ± 1.776
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here