Amino acid dipepetide frequency for Acetobacter sp. CAG:977

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.977AlaAla: 9.977 ± 0.176	1.251AlaCys: 1.251 ± 0.049	5.768AlaAsp: 5.768 ± 0.099	7.195AlaGlu: 7.195 ± 0.132	4.487AlaPhe: 4.487 ± 0.108	6.519AlaGly: 6.519 ± 0.111	1.343AlaHis: 1.343 ± 0.051	3.292AlaIle: 3.292 ± 0.092	5.942AlaLys: 5.942 ± 0.105	10.334AlaLeu: 10.334 ± 0.164	2.293AlaMet: 2.293 ± 0.067	2.613AlaAsn: 2.613 ± 0.075	3.677AlaPro: 3.677 ± 0.093	3.27AlaGln: 3.27 ± 0.08	4.582AlaArg: 4.582 ± 0.093	5.28AlaSer: 5.28 ± 0.103	3.282AlaThr: 3.282 ± 0.083	8.338AlaVal: 8.338 ± 0.16	0.771AlaTrp: 0.771 ± 0.037	2.655AlaTyr: 2.655 ± 0.075	0.003AlaXaa: 0.003 ± 0.002
Cys	1.2CysAla: 1.2 ± 0.05	0.191CysCys: 0.191 ± 0.021	0.686CysAsp: 0.686 ± 0.04	0.719CysGlu: 0.719 ± 0.035	0.633CysPhe: 0.633 ± 0.038	1.219CysGly: 1.219 ± 0.051	0.212CysHis: 0.212 ± 0.02	0.669CysIle: 0.669 ± 0.036	0.543CysLys: 0.543 ± 0.038	1.205CysLeu: 1.205 ± 0.049	0.228CysMet: 0.228 ± 0.017	0.371CysAsn: 0.371 ± 0.026	0.74CysPro: 0.74 ± 0.045	0.343CysGln: 0.343 ± 0.024	0.858CysArg: 0.858 ± 0.047	0.891CysSer: 0.891 ± 0.05	0.6CysThr: 0.6 ± 0.039	0.914CysVal: 0.914 ± 0.044	0.079CysTrp: 0.079 ± 0.012	0.35CysTyr: 0.35 ± 0.025	0.002CysXaa: 0.002 ± 0.002
Asp	5.728AspAla: 5.728 ± 0.095	0.598AspCys: 0.598 ± 0.04	3.122AspAsp: 3.122 ± 0.086	4.046AspGlu: 4.046 ± 0.092	3.061AspPhe: 3.061 ± 0.08	4.492AspGly: 4.492 ± 0.131	0.66AspHis: 0.66 ± 0.039	3.766AspIle: 3.766 ± 0.095	3.675AspLys: 3.675 ± 0.084	5.461AspLeu: 5.461 ± 0.107	1.676AspMet: 1.676 ± 0.054	1.924AspAsn: 1.924 ± 0.055	2.515AspPro: 2.515 ± 0.064	1.115AspGln: 1.115 ± 0.049	2.751AspArg: 2.751 ± 0.07	2.749AspSer: 2.749 ± 0.077	2.796AspThr: 2.796 ± 0.065	4.246AspVal: 4.246 ± 0.071	0.629AspTrp: 0.629 ± 0.032	1.77AspTyr: 1.77 ± 0.055	0.002AspXaa: 0.002 ± 0.002
Glu	6.28GluAla: 6.28 ± 0.119	0.59GluCys: 0.59 ± 0.032	3.527GluAsp: 3.527 ± 0.08	5.57GluGlu: 5.57 ± 0.14	2.331GluPhe: 2.331 ± 0.063	4.104GluGly: 4.104 ± 0.086	1.174GluHis: 1.174 ± 0.047	4.882GluIle: 4.882 ± 0.092	6.38GluLys: 6.38 ± 0.13	5.461GluLeu: 5.461 ± 0.103	2.156GluMet: 2.156 ± 0.06	4.042GluAsn: 4.042 ± 0.085	2.205GluPro: 2.205 ± 0.06	2.558GluGln: 2.558 ± 0.076	4.471GluArg: 4.471 ± 0.124	2.934GluSer: 2.934 ± 0.082	4.828GluThr: 4.828 ± 0.118	3.399GluVal: 3.399 ± 0.086	0.605GluTrp: 0.605 ± 0.031	1.938GluTyr: 1.938 ± 0.064	0.002GluXaa: 0.002 ± 0.002
Phe	3.947PheAla: 3.947 ± 0.097	0.831PheCys: 0.831 ± 0.037	2.929PheAsp: 2.929 ± 0.07	2.727PheGlu: 2.727 ± 0.079	2.741PhePhe: 2.741 ± 0.091	3.318PheGly: 3.318 ± 0.098	0.634PheHis: 0.634 ± 0.032	2.587PheIle: 2.587 ± 0.072	2.78PheLys: 2.78 ± 0.066	4.768PheLeu: 4.768 ± 0.113	1.248PheMet: 1.248 ± 0.046	1.686PheAsn: 1.686 ± 0.058	1.948PhePro: 1.948 ± 0.056	1.076PheGln: 1.076 ± 0.041	1.948PheArg: 1.948 ± 0.062	3.853PheSer: 3.853 ± 0.084	1.919PheThr: 1.919 ± 0.061	3.184PheVal: 3.184 ± 0.087	0.527PheTrp: 0.527 ± 0.034	1.52PheTyr: 1.52 ± 0.055	0.002PheXaa: 0.002 ± 0.002
Gly	5.892GlyAla: 5.892 ± 0.118	1.074GlyCys: 1.074 ± 0.054	3.479GlyAsp: 3.479 ± 0.079	4.284GlyGlu: 4.284 ± 0.099	3.156GlyPhe: 3.156 ± 0.075	4.909GlyGly: 4.909 ± 0.136	1.262GlyHis: 1.262 ± 0.042	4.832GlyIle: 4.832 ± 0.1	5.416GlyLys: 5.416 ± 0.099	6.244GlyLeu: 6.244 ± 0.115	2.131GlyMet: 2.131 ± 0.066	2.896GlyAsn: 2.896 ± 0.092	1.296GlyPro: 1.296 ± 0.058	1.991GlyGln: 1.991 ± 0.062	3.854GlyArg: 3.854 ± 0.108	3.713GlySer: 3.713 ± 0.097	3.973GlyThr: 3.973 ± 0.103	4.927GlyVal: 4.927 ± 0.096	0.826GlyTrp: 0.826 ± 0.042	2.468GlyTyr: 2.468 ± 0.078	0.005GlyXaa: 0.005 ± 0.003
His	1.295HisAla: 1.295 ± 0.047	0.262HisCys: 0.262 ± 0.024	0.893HisAsp: 0.893 ± 0.04	0.939HisGlu: 0.939 ± 0.038	0.703HisPhe: 0.703 ± 0.038	1.105HisGly: 1.105 ± 0.047	0.345HisHis: 0.345 ± 0.027	1.014HisIle: 1.014 ± 0.047	0.838HisLys: 0.838 ± 0.036	1.486HisLeu: 1.486 ± 0.054	0.315HisMet: 0.315 ± 0.026	0.564HisAsn: 0.564 ± 0.031	1.053HisPro: 1.053 ± 0.047	0.422HisGln: 0.422 ± 0.026	0.817HisArg: 0.817 ± 0.039	0.927HisSer: 0.927 ± 0.042	0.772HisThr: 0.772 ± 0.039	0.917HisVal: 0.917 ± 0.043	0.203HisTrp: 0.203 ± 0.019	0.498HisTyr: 0.498 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.278IleAla: 5.278 ± 0.102	0.814IleCys: 0.814 ± 0.04	3.542IleAsp: 3.542 ± 0.077	3.76IleGlu: 3.76 ± 0.092	2.487IlePhe: 2.487 ± 0.079	3.991IleGly: 3.991 ± 0.113	0.867IleHis: 0.867 ± 0.039	3.058IleIle: 3.058 ± 0.083	3.673IleLys: 3.673 ± 0.081	5.588IleLeu: 5.588 ± 0.117	1.22IleMet: 1.22 ± 0.046	2.219IleAsn: 2.219 ± 0.065	2.836IlePro: 2.836 ± 0.078	1.524IleGln: 1.524 ± 0.049	3.296IleArg: 3.296 ± 0.076	4.259IleSer: 4.259 ± 0.08	2.732IleThr: 2.732 ± 0.077	3.694IleVal: 3.694 ± 0.091	0.417IleTrp: 0.417 ± 0.03	1.588IleTyr: 1.588 ± 0.053	0.002IleXaa: 0.002 ± 0.002
Lys	6.538LysAla: 6.538 ± 0.126	0.565LysCys: 0.565 ± 0.043	4.104LysAsp: 4.104 ± 0.092	5.604LysGlu: 5.604 ± 0.118	1.87LysPhe: 1.87 ± 0.057	4.282LysGly: 4.282 ± 0.109	0.964LysHis: 0.964 ± 0.042	4.914LysIle: 4.914 ± 0.114	6.119LysLys: 6.119 ± 0.16	4.866LysLeu: 4.866 ± 0.098	2.07LysMet: 2.07 ± 0.061	3.873LysAsn: 3.873 ± 0.093	2.558LysPro: 2.558 ± 0.076	2.206LysGln: 2.206 ± 0.07	3.696LysArg: 3.696 ± 0.091	3.847LysSer: 3.847 ± 0.09	4.787LysThr: 4.787 ± 0.111	3.644LysVal: 3.644 ± 0.083	0.536LysTrp: 0.536 ± 0.033	1.879LysTyr: 1.879 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	7.905LeuAla: 7.905 ± 0.135	1.451LeuCys: 1.451 ± 0.058	5.068LeuAsp: 5.068 ± 0.079	5.616LeuGlu: 5.616 ± 0.117	5.009LeuPhe: 5.009 ± 0.13	5.911LeuGly: 5.911 ± 0.119	1.469LeuHis: 1.469 ± 0.05	5.58LeuIle: 5.58 ± 0.11	7.483LeuLys: 7.483 ± 0.11	8.753LeuLeu: 8.753 ± 0.164	2.599LeuMet: 2.599 ± 0.068	4.401LeuAsn: 4.401 ± 0.089	5.361LeuPro: 5.361 ± 0.102	2.624LeuGln: 2.624 ± 0.062	4.794LeuArg: 4.794 ± 0.097	7.724LeuSer: 7.724 ± 0.135	5.252LeuThr: 5.252 ± 0.105	4.67LeuVal: 4.67 ± 0.101	0.862LeuTrp: 0.862 ± 0.05	2.758LeuTyr: 2.758 ± 0.067	0.003LeuXaa: 0.003 ± 0.003
Met	2.462MetAla: 2.462 ± 0.062	0.226MetCys: 0.226 ± 0.022	1.286MetAsp: 1.286 ± 0.048	1.603MetGlu: 1.603 ± 0.051	1.101MetPhe: 1.101 ± 0.038	1.577MetGly: 1.577 ± 0.056	0.441MetHis: 0.441 ± 0.027	1.505MetIle: 1.505 ± 0.049	1.989MetLys: 1.989 ± 0.063	2.493MetLeu: 2.493 ± 0.075	0.843MetMet: 0.843 ± 0.037	1.131MetAsn: 1.131 ± 0.043	1.672MetPro: 1.672 ± 0.056	1.058MetGln: 1.058 ± 0.04	1.6MetArg: 1.6 ± 0.063	1.862MetSer: 1.862 ± 0.058	1.882MetThr: 1.882 ± 0.057	1.443MetVal: 1.443 ± 0.056	0.19MetTrp: 0.19 ± 0.017	0.59MetTyr: 0.59 ± 0.033	0.002MetXaa: 0.002 ± 0.002
Asn	4.38AsnAla: 4.38 ± 0.09	0.427AsnCys: 0.427 ± 0.04	2.248AsnAsp: 2.248 ± 0.067	2.582AsnGlu: 2.582 ± 0.072	1.536AsnPhe: 1.536 ± 0.046	3.601AsnGly: 3.601 ± 0.088	0.543AsnHis: 0.543 ± 0.032	2.524AsnIle: 2.524 ± 0.076	2.344AsnLys: 2.344 ± 0.061	3.48AsnLeu: 3.48 ± 0.076	1.134AsnMet: 1.134 ± 0.042	1.326AsnAsn: 1.326 ± 0.05	2.296AsnPro: 2.296 ± 0.073	1.048AsnGln: 1.048 ± 0.042	2.039AsnArg: 2.039 ± 0.049	2.027AsnSer: 2.027 ± 0.06	1.81AsnThr: 1.81 ± 0.064	3.006AsnVal: 3.006 ± 0.07	0.403AsnTrp: 0.403 ± 0.027	1.057AsnTyr: 1.057 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	4.073ProAla: 4.073 ± 0.084	0.479ProCys: 0.479 ± 0.035	3.158ProAsp: 3.158 ± 0.077	4.435ProGlu: 4.435 ± 0.098	2.301ProPhe: 2.301 ± 0.067	2.02ProGly: 2.02 ± 0.064	0.684ProHis: 0.684 ± 0.04	1.682ProIle: 1.682 ± 0.059	2.653ProLys: 2.653 ± 0.064	4.099ProLeu: 4.099 ± 0.089	1.038ProMet: 1.038 ± 0.04	1.589ProAsn: 1.589 ± 0.053	1.97ProPro: 1.97 ± 0.082	1.638ProGln: 1.638 ± 0.057	1.65ProArg: 1.65 ± 0.057	2.922ProSer: 2.922 ± 0.075	1.874ProThr: 1.874 ± 0.059	4.301ProVal: 4.301 ± 0.09	0.396ProTrp: 0.396 ± 0.026	1.577ProTyr: 1.577 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	2.998GlnAla: 2.998 ± 0.074	0.25GlnCys: 0.25 ± 0.023	1.557GlnAsp: 1.557 ± 0.054	2.251GlnGlu: 2.251 ± 0.072	0.86GlnPhe: 0.86 ± 0.04	1.955GlnGly: 1.955 ± 0.062	0.417GlnHis: 0.417 ± 0.026	2.005GlnIle: 2.005 ± 0.048	2.936GlnLys: 2.936 ± 0.082	2.063GlnLeu: 2.063 ± 0.058	0.945GlnMet: 0.945 ± 0.039	1.648GlnAsn: 1.648 ± 0.055	1.21GlnPro: 1.21 ± 0.05	1.146GlnGln: 1.146 ± 0.08	1.526GlnArg: 1.526 ± 0.053	1.829GlnSer: 1.829 ± 0.059	2.05GlnThr: 2.05 ± 0.066	1.631GlnVal: 1.631 ± 0.06	0.222GlnTrp: 0.222 ± 0.019	0.784GlnTyr: 0.784 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	4.159ArgAla: 4.159 ± 0.093	0.562ArgCys: 0.562 ± 0.038	2.668ArgAsp: 2.668 ± 0.069	3.625ArgGlu: 3.625 ± 0.086	2.767ArgPhe: 2.767 ± 0.076	2.863ArgGly: 2.863 ± 0.087	0.931ArgHis: 0.931 ± 0.04	3.477ArgIle: 3.477 ± 0.075	3.353ArgLys: 3.353 ± 0.077	6.094ArgLeu: 6.094 ± 0.138	1.658ArgMet: 1.658 ± 0.051	2.025ArgAsn: 2.025 ± 0.058	2.318ArgPro: 2.318 ± 0.067	1.819ArgGln: 1.819 ± 0.055	3.785ArgArg: 3.785 ± 0.111	3.07ArgSer: 3.07 ± 0.069	2.486ArgThr: 2.486 ± 0.061	3.18ArgVal: 3.18 ± 0.088	0.512ArgTrp: 0.512 ± 0.03	1.753ArgTyr: 1.753 ± 0.058	0.003ArgXaa: 0.003 ± 0.003
Ser	6.192SerAla: 6.192 ± 0.112	0.865SerCys: 0.865 ± 0.049	3.882SerAsp: 3.882 ± 0.086	4.64SerGlu: 4.64 ± 0.114	3.454SerPhe: 3.454 ± 0.084	5.226SerGly: 5.226 ± 0.104	0.919SerHis: 0.919 ± 0.04	2.605SerIle: 2.605 ± 0.065	3.587SerLys: 3.587 ± 0.075	6.404SerLeu: 6.404 ± 0.119	1.401SerMet: 1.401 ± 0.051	1.839SerAsn: 1.839 ± 0.053	2.687SerPro: 2.687 ± 0.068	1.746SerGln: 1.746 ± 0.055	3.098SerArg: 3.098 ± 0.068	4.239SerSer: 4.239 ± 0.095	2.32SerThr: 2.32 ± 0.052	5.316SerVal: 5.316 ± 0.106	0.622SerTrp: 0.622 ± 0.031	2.072SerTyr: 2.072 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	5.321ThrAla: 5.321 ± 0.109	0.529ThrCys: 0.529 ± 0.04	3.148ThrAsp: 3.148 ± 0.077	3.322ThrGlu: 3.322 ± 0.087	2.134ThrPhe: 2.134 ± 0.069	4.406ThrGly: 4.406 ± 0.093	0.779ThrHis: 0.779 ± 0.041	2.296ThrIle: 2.296 ± 0.071	2.653ThrLys: 2.653 ± 0.066	5.18ThrLeu: 5.18 ± 0.107	1.081ThrMet: 1.081 ± 0.049	1.688ThrAsn: 1.688 ± 0.059	3.077ThrPro: 3.077 ± 0.076	1.576ThrGln: 1.576 ± 0.057	2.194ThrArg: 2.194 ± 0.06	2.939ThrSer: 2.939 ± 0.079	2.287ThrThr: 2.287 ± 0.067	4.646ThrVal: 4.646 ± 0.11	0.353ThrTrp: 0.353 ± 0.026	1.422ThrTyr: 1.422 ± 0.06	0.002ThrXaa: 0.002 ± 0.002
Val	5.538ValAla: 5.538 ± 0.105	1.188ValCys: 1.188 ± 0.055	3.273ValAsp: 3.273 ± 0.082	3.951ValGlu: 3.951 ± 0.1	3.62ValPhe: 3.62 ± 0.094	4.218ValGly: 4.218 ± 0.118	1.02ValHis: 1.02 ± 0.044	4.08ValIle: 4.08 ± 0.085	4.197ValLys: 4.197 ± 0.089	7.638ValLeu: 7.638 ± 0.126	1.888ValMet: 1.888 ± 0.05	2.375ValAsn: 2.375 ± 0.066	3.379ValPro: 3.379 ± 0.08	1.805ValGln: 1.805 ± 0.053	3.873ValArg: 3.873 ± 0.086	5.757ValSer: 5.757 ± 0.102	3.458ValThr: 3.458 ± 0.094	4.558ValVal: 4.558 ± 0.106	0.762ValTrp: 0.762 ± 0.036	2.062ValTyr: 2.062 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.693TrpAla: 0.693 ± 0.034	0.112TrpCys: 0.112 ± 0.014	0.507TrpAsp: 0.507 ± 0.033	0.605TrpGlu: 0.605 ± 0.032	0.457TrpPhe: 0.457 ± 0.029	0.683TrpGly: 0.683 ± 0.038	0.21TrpHis: 0.21 ± 0.023	0.526TrpIle: 0.526 ± 0.038	0.658TrpLys: 0.658 ± 0.034	0.976TrpLeu: 0.976 ± 0.041	0.272TrpMet: 0.272 ± 0.019	0.45TrpAsn: 0.45 ± 0.026	0.262TrpPro: 0.262 ± 0.023	0.39TrpGln: 0.39 ± 0.029	0.59TrpArg: 0.59 ± 0.035	0.469TrpSer: 0.469 ± 0.029	0.529TrpThr: 0.529 ± 0.031	0.562TrpVal: 0.562 ± 0.034	0.117TrpTrp: 0.117 ± 0.013	0.309TrpTyr: 0.309 ± 0.023	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.006TyrAla: 3.006 ± 0.07	0.383TyrCys: 0.383 ± 0.026	1.938TyrAsp: 1.938 ± 0.062	1.962TyrGlu: 1.962 ± 0.059	1.46TyrPhe: 1.46 ± 0.056	2.227TyrGly: 2.227 ± 0.074	0.493TyrHis: 0.493 ± 0.03	1.55TyrIle: 1.55 ± 0.048	1.705TyrLys: 1.705 ± 0.056	2.701TyrLeu: 2.701 ± 0.061	0.757TyrMet: 0.757 ± 0.04	1.153TyrAsn: 1.153 ± 0.048	1.479TyrPro: 1.479 ± 0.052	0.929TyrGln: 0.929 ± 0.039	1.682TyrArg: 1.682 ± 0.051	1.824TyrSer: 1.824 ± 0.056	1.395TyrThr: 1.395 ± 0.051	2.063TyrVal: 2.063 ± 0.062	0.343TyrTrp: 0.343 ± 0.028	1.05TyrTyr: 1.05 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.009XaaAla: 0.009 ± 0.004	0.002XaaCys: 0.002 ± 0.002	0.002XaaAsp: 0.002 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.003XaaPhe: 0.003 ± 0.003	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.002	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.005XaaArg: 0.005 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.031XaaXaa: 0.031 ± 0.01

Statistics based on 1775 proteins (580122 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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