Verruconis gallopava

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D1YJZ4|A0A0D1YJZ4_9PEZI 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Verruconis gallopava OX=253628 GN=PV09_07469 PE=3 SV=1
MM1 pKa = 7.38YY2 pKa = 9.13MLKK5 pKa = 10.31SVVSIALFTAYY16 pKa = 10.19AIAQSTLQFTSTPQTVQAGQSYY38 pKa = 7.98TLTWTGGDD46 pKa = 3.18STQPVTILLRR56 pKa = 11.84NGDD59 pKa = 4.04PSDD62 pKa = 3.66LQTIGTLTTTATGGSFTWTVDD83 pKa = 3.3PSIADD88 pKa = 3.45SNQYY92 pKa = 10.79AFEE95 pKa = 4.13IVQGSEE101 pKa = 4.08TNYY104 pKa = 10.47SNQFTVTGGSGTLSSATGTATISASASASASASAAASTTEE144 pKa = 4.14SSSGTSSTTEE154 pKa = 3.75SSSASASASSTTLSSAAASTTSTTSSAKK182 pKa = 9.75TSATAATSAPSSGASGLQSPIALILGAVAAMLSFAA217 pKa = 5.1

Molecular weight:
21.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D1X935|A0A0D1X935_9PEZI Uncharacterized protein OS=Verruconis gallopava OX=253628 GN=PV09_09619 PE=3 SV=1
MM1 pKa = 8.03AIPEE5 pKa = 4.25TRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 10.03FPGHH13 pKa = 5.42QLLSRR18 pKa = 11.84FNNVLHH24 pKa = 6.33QYY26 pKa = 7.6EE27 pKa = 4.06ICHH30 pKa = 5.88RR31 pKa = 11.84QFPDD35 pKa = 3.31GRR37 pKa = 11.84SSGLLPDD44 pKa = 3.6WLDD47 pKa = 3.3ITGTNAKK54 pKa = 10.07FGGWNTTPSNAFWSSGDD71 pKa = 3.55LDD73 pKa = 3.52PWRR76 pKa = 11.84SQTVFSEE83 pKa = 4.69QPSSPQYY90 pKa = 10.91KK91 pKa = 7.77ITQNIRR97 pKa = 11.84ACGQSSRR104 pKa = 11.84NAKK107 pKa = 10.47DD108 pKa = 3.81EE109 pKa = 4.1IFGPLCRR116 pKa = 11.84STLLISFHH124 pKa = 6.65RR125 pKa = 11.84FRR127 pKa = 11.84PVHH130 pKa = 6.01RR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84IFGRR137 pKa = 11.84EE138 pKa = 3.26RR139 pKa = 11.84WKK141 pKa = 11.01NGCDD145 pKa = 5.09AGSLWQTEE153 pKa = 4.1SRR155 pKa = 11.84KK156 pKa = 9.9RR157 pKa = 11.84RR158 pKa = 11.84VSQKK162 pKa = 9.13VAIYY166 pKa = 10.51

Molecular weight:
19.27 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9813

955

10768

5262068

51

6703

488.7

54.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.731 ± 0.019

1.23 ± 0.01

5.62 ± 0.017

6.337 ± 0.021

3.698 ± 0.014

6.621 ± 0.021

2.367 ± 0.01

4.926 ± 0.016

5.171 ± 0.022

8.811 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.008

3.716 ± 0.011

5.866 ± 0.023

4.026 ± 0.015

6.294 ± 0.021

8.335 ± 0.029

5.774 ± 0.013

6.048 ± 0.014

1.438 ± 0.009

2.76 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski