Verruconis gallopava
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10768 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D1YJZ4|A0A0D1YJZ4_9PEZI 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Verruconis gallopava OX=253628 GN=PV09_07469 PE=3 SV=1
MM1 pKa = 7.38 YY2 pKa = 9.13 MLKK5 pKa = 10.31 SVVSIALFTAYY16 pKa = 10.19 AIAQSTLQFTSTPQTVQAGQSYY38 pKa = 7.98 TLTWTGGDD46 pKa = 3.18 STQPVTILLRR56 pKa = 11.84 NGDD59 pKa = 4.04 PSDD62 pKa = 3.66 LQTIGTLTTTATGGSFTWTVDD83 pKa = 3.3 PSIADD88 pKa = 3.45 SNQYY92 pKa = 10.79 AFEE95 pKa = 4.13 IVQGSEE101 pKa = 4.08 TNYY104 pKa = 10.47 SNQFTVTGGSGTLSSATGTATISASASASASASAAASTTEE144 pKa = 4.14 SSSGTSSTTEE154 pKa = 3.75 SSSASASASSTTLSSAAASTTSTTSSAKK182 pKa = 9.75 TSATAATSAPSSGASGLQSPIALILGAVAAMLSFAA217 pKa = 5.1
Molecular weight: 21.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 0.604
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A0D1X935|A0A0D1X935_9PEZI Uncharacterized protein OS=Verruconis gallopava OX=253628 GN=PV09_09619 PE=3 SV=1
MM1 pKa = 8.03 AIPEE5 pKa = 4.25 TRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 10.03 FPGHH13 pKa = 5.42 QLLSRR18 pKa = 11.84 FNNVLHH24 pKa = 6.33 QYY26 pKa = 7.6 EE27 pKa = 4.06 ICHH30 pKa = 5.88 RR31 pKa = 11.84 QFPDD35 pKa = 3.31 GRR37 pKa = 11.84 SSGLLPDD44 pKa = 3.6 WLDD47 pKa = 3.3 ITGTNAKK54 pKa = 10.07 FGGWNTTPSNAFWSSGDD71 pKa = 3.55 LDD73 pKa = 3.52 PWRR76 pKa = 11.84 SQTVFSEE83 pKa = 4.69 QPSSPQYY90 pKa = 10.91 KK91 pKa = 7.77 ITQNIRR97 pKa = 11.84 ACGQSSRR104 pKa = 11.84 NAKK107 pKa = 10.47 DD108 pKa = 3.81 EE109 pKa = 4.1 IFGPLCRR116 pKa = 11.84 STLLISFHH124 pKa = 6.65 RR125 pKa = 11.84 FRR127 pKa = 11.84 PVHH130 pKa = 6.01 RR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 IFGRR137 pKa = 11.84 EE138 pKa = 3.26 RR139 pKa = 11.84 WKK141 pKa = 11.01 NGCDD145 pKa = 5.09 AGSLWQTEE153 pKa = 4.1 SRR155 pKa = 11.84 KK156 pKa = 9.9 RR157 pKa = 11.84 RR158 pKa = 11.84 VSQKK162 pKa = 9.13 VAIYY166 pKa = 10.51
Molecular weight: 19.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.56
IPC_protein 10.379
Toseland 10.672
ProMoST 10.409
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.921
Grimsley 10.804
Solomon 10.906
Lehninger 10.862
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.657
IPC_peptide 10.906
IPC2_peptide 9.706
IPC2.peptide.svr19 8.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9813
955
10768
5262068
51
6703
488.7
54.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.731 ± 0.019
1.23 ± 0.01
5.62 ± 0.017
6.337 ± 0.021
3.698 ± 0.014
6.621 ± 0.021
2.367 ± 0.01
4.926 ± 0.016
5.171 ± 0.022
8.811 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.229 ± 0.008
3.716 ± 0.011
5.866 ± 0.023
4.026 ± 0.015
6.294 ± 0.021
8.335 ± 0.029
5.774 ± 0.013
6.048 ± 0.014
1.438 ± 0.009
2.76 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here