Amino acid dipepetide frequency for Pseudomonas sp. 21C1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.927AlaAla: 13.927 ± 0.137
1.356AlaCys: 1.356 ± 0.034
6.264AlaAsp: 6.264 ± 0.063
7.613AlaGlu: 7.613 ± 0.088
3.974AlaPhe: 3.974 ± 0.057
9.479AlaGly: 9.479 ± 0.116
2.356AlaHis: 2.356 ± 0.047
5.281AlaIle: 5.281 ± 0.067
3.355AlaLys: 3.355 ± 0.059
15.108AlaLeu: 15.108 ± 0.129
2.888AlaMet: 2.888 ± 0.049
2.976AlaAsn: 2.976 ± 0.052
4.991AlaPro: 4.991 ± 0.072
5.734AlaGln: 5.734 ± 0.069
7.635AlaArg: 7.635 ± 0.099
6.622AlaSer: 6.622 ± 0.08
4.946AlaThr: 4.946 ± 0.068
7.73AlaVal: 7.73 ± 0.08
1.69AlaTrp: 1.69 ± 0.035
2.701AlaTyr: 2.701 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.215CysAla: 1.215 ± 0.028
0.142CysCys: 0.142 ± 0.01
0.504CysAsp: 0.504 ± 0.018
0.571CysGlu: 0.571 ± 0.023
0.335CysPhe: 0.335 ± 0.014
0.912CysGly: 0.912 ± 0.025
0.267CysHis: 0.267 ± 0.013
0.434CysIle: 0.434 ± 0.019
0.264CysLys: 0.264 ± 0.015
1.174CysLeu: 1.174 ± 0.036
0.191CysMet: 0.191 ± 0.01
0.245CysAsn: 0.245 ± 0.014
0.508CysPro: 0.508 ± 0.021
0.404CysGln: 0.404 ± 0.016
0.621CysArg: 0.621 ± 0.024
0.632CysSer: 0.632 ± 0.02
0.412CysThr: 0.412 ± 0.016
0.688CysVal: 0.688 ± 0.021
0.159CysTrp: 0.159 ± 0.011
0.243CysTyr: 0.243 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.217AspAla: 6.217 ± 0.07
0.525AspCys: 0.525 ± 0.022
2.882AspAsp: 2.882 ± 0.053
3.468AspGlu: 3.468 ± 0.049
2.009AspPhe: 2.009 ± 0.041
4.563AspGly: 4.563 ± 0.072
1.06AspHis: 1.06 ± 0.027
2.581AspIle: 2.581 ± 0.05
1.747AspLys: 1.747 ± 0.032
6.031AspLeu: 6.031 ± 0.071
1.174AspMet: 1.174 ± 0.027
1.584AspAsn: 1.584 ± 0.043
2.68AspPro: 2.68 ± 0.041
2.081AspGln: 2.081 ± 0.044
2.876AspArg: 2.876 ± 0.049
3.053AspSer: 3.053 ± 0.051
2.432AspThr: 2.432 ± 0.051
3.407AspVal: 3.407 ± 0.055
0.947AspTrp: 0.947 ± 0.027
1.782AspTyr: 1.782 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.839GluAla: 6.839 ± 0.071
0.406GluCys: 0.406 ± 0.017
2.469GluAsp: 2.469 ± 0.044
2.884GluGlu: 2.884 ± 0.056
1.696GluPhe: 1.696 ± 0.038
3.873GluGly: 3.873 ± 0.058
1.602GluHis: 1.602 ± 0.035
2.61GluIle: 2.61 ± 0.043
1.835GluLys: 1.835 ± 0.035
7.226GluLeu: 7.226 ± 0.081
1.249GluMet: 1.249 ± 0.03
1.41GluAsn: 1.41 ± 0.029
2.505GluPro: 2.505 ± 0.048
4.185GluGln: 4.185 ± 0.068
5.106GluArg: 5.106 ± 0.075
2.555GluSer: 2.555 ± 0.043
2.451GluThr: 2.451 ± 0.04
4.336GluVal: 4.336 ± 0.066
0.714GluTrp: 0.714 ± 0.023
1.152GluTyr: 1.152 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.334PheAla: 4.334 ± 0.057
0.43PheCys: 0.43 ± 0.018
2.369PheAsp: 2.369 ± 0.033
2.064PheGlu: 2.064 ± 0.036
1.367PhePhe: 1.367 ± 0.036
3.071PheGly: 3.071 ± 0.05
0.673PheHis: 0.673 ± 0.023
1.802PheIle: 1.802 ± 0.035
1.144PheLys: 1.144 ± 0.028
3.126PheLeu: 3.126 ± 0.047
0.81PheMet: 0.81 ± 0.024
1.294PheAsn: 1.294 ± 0.027
1.292PhePro: 1.292 ± 0.031
1.198PheGln: 1.198 ± 0.025
1.772PheArg: 1.772 ± 0.038
2.52PheSer: 2.52 ± 0.044
1.868PheThr: 1.868 ± 0.036
2.491PheVal: 2.491 ± 0.041
0.519PheTrp: 0.519 ± 0.017
1.018PheTyr: 1.018 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.693GlyAla: 7.693 ± 0.078
0.916GlyCys: 0.916 ± 0.025
3.97GlyAsp: 3.97 ± 0.056
4.981GlyGlu: 4.981 ± 0.067
3.281GlyPhe: 3.281 ± 0.051
6.061GlyGly: 6.061 ± 0.108
1.797GlyHis: 1.797 ± 0.033
4.056GlyIle: 4.056 ± 0.055
3.078GlyLys: 3.078 ± 0.055
9.746GlyLeu: 9.746 ± 0.088
2.184GlyMet: 2.184 ± 0.046
2.404GlyAsn: 2.404 ± 0.07
2.502GlyPro: 2.502 ± 0.045
3.843GlyGln: 3.843 ± 0.052
4.905GlyArg: 4.905 ± 0.061
4.646GlySer: 4.646 ± 0.08
3.702GlyThr: 3.702 ± 0.064
6.033GlyVal: 6.033 ± 0.069
1.322GlyTrp: 1.322 ± 0.035
2.468GlyTyr: 2.468 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.406HisAla: 2.406 ± 0.041
0.344HisCys: 0.344 ± 0.016
1.173HisAsp: 1.173 ± 0.027
1.166HisGlu: 1.166 ± 0.031
0.894HisPhe: 0.894 ± 0.025
1.886HisGly: 1.886 ± 0.034
0.607HisHis: 0.607 ± 0.024
0.963HisIle: 0.963 ± 0.025
0.623HisLys: 0.623 ± 0.023
2.77HisLeu: 2.77 ± 0.05
0.451HisMet: 0.451 ± 0.017
0.654HisAsn: 0.654 ± 0.022
1.361HisPro: 1.361 ± 0.039
0.92HisGln: 0.92 ± 0.025
1.265HisArg: 1.265 ± 0.028
1.285HisSer: 1.285 ± 0.03
0.952HisThr: 0.952 ± 0.028
1.249HisVal: 1.249 ± 0.033
0.455HisTrp: 0.455 ± 0.016
0.729HisTyr: 0.729 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.869IleAla: 5.869 ± 0.071
0.502IleCys: 0.502 ± 0.017
3.2IleAsp: 3.2 ± 0.045
3.271IleGlu: 3.271 ± 0.048
1.345IlePhe: 1.345 ± 0.033
4.469IleGly: 4.469 ± 0.058
0.901IleHis: 0.901 ± 0.026
2.003IleIle: 2.003 ± 0.042
1.6IleLys: 1.6 ± 0.038
4.06IleLeu: 4.06 ± 0.051
0.799IleMet: 0.799 ± 0.025
1.684IleAsn: 1.684 ± 0.04
2.087IlePro: 2.087 ± 0.033
1.619IleGln: 1.619 ± 0.035
2.799IleArg: 2.799 ± 0.038
2.926IleSer: 2.926 ± 0.054
2.453IleThr: 2.453 ± 0.047
3.024IleVal: 3.024 ± 0.049
0.486IleTrp: 0.486 ± 0.019
1.059IleTyr: 1.059 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.731LysAla: 3.731 ± 0.057
0.156LysCys: 0.156 ± 0.011
1.523LysAsp: 1.523 ± 0.04
1.419LysGlu: 1.419 ± 0.039
0.761LysPhe: 0.761 ± 0.024
2.285LysGly: 2.285 ± 0.045
0.677LysHis: 0.677 ± 0.023
1.399LysIle: 1.399 ± 0.033
1.103LysLys: 1.103 ± 0.032
3.373LysLeu: 3.373 ± 0.062
0.614LysMet: 0.614 ± 0.023
0.872LysAsn: 0.872 ± 0.028
1.82LysPro: 1.82 ± 0.041
1.478LysGln: 1.478 ± 0.038
2.229LysArg: 2.229 ± 0.038
1.555LysSer: 1.555 ± 0.034
1.674LysThr: 1.674 ± 0.039
2.437LysVal: 2.437 ± 0.044
0.312LysTrp: 0.312 ± 0.014
0.621LysTyr: 0.621 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.59LeuAla: 15.59 ± 0.133
1.321LeuCys: 1.321 ± 0.028
7.097LeuAsp: 7.097 ± 0.082
6.425LeuGlu: 6.425 ± 0.074
4.326LeuPhe: 4.326 ± 0.062
9.7LeuGly: 9.7 ± 0.09
2.546LeuHis: 2.546 ± 0.043
5.605LeuIle: 5.605 ± 0.063
3.916LeuLys: 3.916 ± 0.061
14.964LeuLeu: 14.964 ± 0.158
2.563LeuMet: 2.563 ± 0.045
3.635LeuAsn: 3.635 ± 0.053
6.541LeuPro: 6.541 ± 0.085
5.422LeuGln: 5.422 ± 0.071
7.911LeuArg: 7.911 ± 0.087
7.625LeuSer: 7.625 ± 0.075
5.912LeuThr: 5.912 ± 0.072
8.063LeuVal: 8.063 ± 0.089
1.44LeuTrp: 1.44 ± 0.035
2.7LeuTyr: 2.7 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.688MetAla: 2.688 ± 0.045
0.156MetCys: 0.156 ± 0.011
0.977MetAsp: 0.977 ± 0.027
0.914MetGlu: 0.914 ± 0.024
0.605MetPhe: 0.605 ± 0.022
1.665MetGly: 1.665 ± 0.039
0.517MetHis: 0.517 ± 0.019
1.043MetIle: 1.043 ± 0.027
0.748MetLys: 0.748 ± 0.021
2.747MetLeu: 2.747 ± 0.049
0.468MetMet: 0.468 ± 0.018
0.754MetAsn: 0.754 ± 0.022
1.386MetPro: 1.386 ± 0.029
1.073MetGln: 1.073 ± 0.027
1.52MetArg: 1.52 ± 0.034
1.633MetSer: 1.633 ± 0.034
1.335MetThr: 1.335 ± 0.032
1.463MetVal: 1.463 ± 0.034
0.161MetTrp: 0.161 ± 0.01
0.331MetTyr: 0.331 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.238AsnAla: 3.238 ± 0.062
0.281AsnCys: 0.281 ± 0.013
1.484AsnAsp: 1.484 ± 0.046
1.368AsnGlu: 1.368 ± 0.03
0.969AsnPhe: 0.969 ± 0.025
2.47AsnGly: 2.47 ± 0.059
0.578AsnHis: 0.578 ± 0.019
1.369AsnIle: 1.369 ± 0.032
0.845AsnLys: 0.845 ± 0.029
3.531AsnLeu: 3.531 ± 0.042
0.577AsnMet: 0.577 ± 0.019
0.932AsnAsn: 0.932 ± 0.033
1.911AsnPro: 1.911 ± 0.036
1.335AsnGln: 1.335 ± 0.032
1.838AsnArg: 1.838 ± 0.035
1.515AsnSer: 1.515 ± 0.04
1.393AsnThr: 1.393 ± 0.034
1.774AsnVal: 1.774 ± 0.041
0.438AsnTrp: 0.438 ± 0.019
0.791AsnTyr: 0.791 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
5.967ProAla: 5.967 ± 0.081
0.366ProCys: 0.366 ± 0.017
2.646ProAsp: 2.646 ± 0.042
3.134ProGlu: 3.134 ± 0.044
1.791ProPhe: 1.791 ± 0.041
3.916ProGly: 3.916 ± 0.061
1.042ProHis: 1.042 ± 0.027
1.873ProIle: 1.873 ± 0.034
1.313ProLys: 1.313 ± 0.032
6.006ProLeu: 6.006 ± 0.073
1.138ProMet: 1.138 ± 0.026
1.236ProAsn: 1.236 ± 0.031
1.948ProPro: 1.948 ± 0.043
2.242ProGln: 2.242 ± 0.044
2.672ProArg: 2.672 ± 0.046
2.626ProSer: 2.626 ± 0.044
2.211ProThr: 2.211 ± 0.037
3.661ProVal: 3.661 ± 0.055
0.789ProTrp: 0.789 ± 0.028
1.172ProTyr: 1.172 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
6.828GlnAla: 6.828 ± 0.079
0.338GlnCys: 0.338 ± 0.014
1.913GlnAsp: 1.913 ± 0.039
1.879GlnGlu: 1.879 ± 0.037
1.369GlnPhe: 1.369 ± 0.031
3.6GlnGly: 3.6 ± 0.055
1.247GlnHis: 1.247 ± 0.031
2.048GlnIle: 2.048 ± 0.038
1.061GlnLys: 1.061 ± 0.026
5.963GlnLeu: 5.963 ± 0.084
1.05GlnMet: 1.05 ± 0.028
1.014GlnAsn: 1.014 ± 0.025
2.608GlnPro: 2.608 ± 0.047
3.056GlnGln: 3.056 ± 0.06
4.18GlnArg: 4.18 ± 0.07
2.317GlnSer: 2.317 ± 0.039
1.996GlnThr: 1.996 ± 0.038
3.989GlnVal: 3.989 ± 0.05
0.708GlnTrp: 0.708 ± 0.02
0.972GlnTyr: 0.972 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
6.466ArgAla: 6.466 ± 0.088
0.637ArgCys: 0.637 ± 0.02
3.61ArgAsp: 3.61 ± 0.047
4.466ArgGlu: 4.466 ± 0.065
2.668ArgPhe: 2.668 ± 0.044
4.174ArgGly: 4.174 ± 0.057
1.745ArgHis: 1.745 ± 0.035
3.273ArgIle: 3.273 ± 0.047
1.827ArgLys: 1.827 ± 0.037
8.982ArgLeu: 8.982 ± 0.083
1.564ArgMet: 1.564 ± 0.036
1.854ArgAsn: 1.854 ± 0.036
2.767ArgPro: 2.767 ± 0.044
3.6ArgGln: 3.6 ± 0.052
4.374ArgArg: 4.374 ± 0.066
3.505ArgSer: 3.505 ± 0.047
2.829ArgThr: 2.829 ± 0.047
4.574ArgVal: 4.574 ± 0.06
1.088ArgTrp: 1.088 ± 0.032
2.095ArgTyr: 2.095 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.537SerAla: 6.537 ± 0.078
0.47SerCys: 0.47 ± 0.016
2.859SerAsp: 2.859 ± 0.049
3.266SerGlu: 3.266 ± 0.045
2.067SerPhe: 2.067 ± 0.039
5.297SerGly: 5.297 ± 0.091
1.235SerHis: 1.235 ± 0.031
2.705SerIle: 2.705 ± 0.049
1.592SerLys: 1.592 ± 0.039
7.451SerLeu: 7.451 ± 0.077
1.307SerMet: 1.307 ± 0.026
1.756SerAsn: 1.756 ± 0.042
2.606SerPro: 2.606 ± 0.043
2.511SerGln: 2.511 ± 0.044
3.664SerArg: 3.664 ± 0.048
3.409SerSer: 3.409 ± 0.06
2.835SerThr: 2.835 ± 0.05
3.901SerVal: 3.901 ± 0.057
0.786SerTrp: 0.786 ± 0.025
1.446SerTyr: 1.446 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.252ThrAla: 5.252 ± 0.074
0.446ThrCys: 0.446 ± 0.021
2.326ThrAsp: 2.326 ± 0.047
2.228ThrGlu: 2.228 ± 0.043
1.749ThrPhe: 1.749 ± 0.035
4.049ThrGly: 4.049 ± 0.068
1.027ThrHis: 1.027 ± 0.029
1.851ThrIle: 1.851 ± 0.044
0.957ThrLys: 0.957 ± 0.027
6.991ThrLeu: 6.991 ± 0.076
0.705ThrMet: 0.705 ± 0.021
1.111ThrAsn: 1.111 ± 0.031
3.116ThrPro: 3.116 ± 0.048
2.095ThrGln: 2.095 ± 0.038
3.094ThrArg: 3.094 ± 0.049
2.591ThrSer: 2.591 ± 0.05
2.353ThrThr: 2.353 ± 0.05
3.212ThrVal: 3.212 ± 0.065
0.747ThrTrp: 0.747 ± 0.026
1.217ThrTyr: 1.217 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
7.872ValAla: 7.872 ± 0.086
0.678ValCys: 0.678 ± 0.021
3.862ValAsp: 3.862 ± 0.059
4.333ValGlu: 4.333 ± 0.057
2.486ValPhe: 2.486 ± 0.036
5.188ValGly: 5.188 ± 0.063
1.358ValHis: 1.358 ± 0.036
3.672ValIle: 3.672 ± 0.056
2.017ValLys: 2.017 ± 0.043
8.535ValLeu: 8.535 ± 0.087
1.66ValMet: 1.66 ± 0.035
2.074ValAsn: 2.074 ± 0.043
3.181ValPro: 3.181 ± 0.047
2.874ValGln: 2.874 ± 0.046
4.42ValArg: 4.42 ± 0.058
4.285ValSer: 4.285 ± 0.064
3.605ValThr: 3.605 ± 0.06
5.326ValVal: 5.326 ± 0.075
0.862ValTrp: 0.862 ± 0.025
1.57ValTyr: 1.57 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.282TrpAla: 1.282 ± 0.027
0.167TrpCys: 0.167 ± 0.01
0.605TrpAsp: 0.605 ± 0.021
0.513TrpGlu: 0.513 ± 0.019
0.512TrpPhe: 0.512 ± 0.02
0.868TrpGly: 0.868 ± 0.028
0.383TrpHis: 0.383 ± 0.021
0.62TrpIle: 0.62 ± 0.018
0.339TrpLys: 0.339 ± 0.016
2.354TrpLeu: 2.354 ± 0.053
0.338TrpMet: 0.338 ± 0.02
0.421TrpAsn: 0.421 ± 0.017
0.673TrpPro: 0.673 ± 0.02
1.14TrpGln: 1.14 ± 0.03
1.169TrpArg: 1.169 ± 0.036
0.795TrpSer: 0.795 ± 0.022
0.601TrpThr: 0.601 ± 0.02
0.916TrpVal: 0.916 ± 0.026
0.228TrpTrp: 0.228 ± 0.015
0.327TrpTyr: 0.327 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.673TyrAla: 2.673 ± 0.05
0.286TyrCys: 0.286 ± 0.012
1.369TyrAsp: 1.369 ± 0.051
1.138TyrGlu: 1.138 ± 0.032
0.96TyrPhe: 0.96 ± 0.025
2.063TyrGly: 2.063 ± 0.038
0.536TyrHis: 0.536 ± 0.02
0.967TyrIle: 0.967 ± 0.027
0.673TyrLys: 0.673 ± 0.023
3.162TyrLeu: 3.162 ± 0.048
0.453TyrMet: 0.453 ± 0.018
0.725TyrAsn: 0.725 ± 0.03
1.27TyrPro: 1.27 ± 0.031
1.324TyrGln: 1.324 ± 0.033
2.048TyrArg: 2.048 ± 0.04
1.607TyrSer: 1.607 ± 0.036
1.157TyrThr: 1.157 ± 0.034
1.583TyrVal: 1.583 ± 0.031
0.401TyrTrp: 0.401 ± 0.014
0.723TyrTyr: 0.723 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4646 proteins (1532859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski