Amino acid dipepetide frequency for Escherichia phage phi467

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.769AlaAla: 8.769 ± 1.528
0.805AlaCys: 0.805 ± 0.226
4.563AlaAsp: 4.563 ± 0.614
6.979AlaGlu: 6.979 ± 0.852
3.311AlaPhe: 3.311 ± 0.645
6.89AlaGly: 6.89 ± 1.162
1.879AlaHis: 1.879 ± 0.394
5.011AlaIle: 5.011 ± 0.67
4.474AlaLys: 4.474 ± 0.793
8.5AlaLeu: 8.5 ± 1.187
2.237AlaMet: 2.237 ± 0.466
3.311AlaAsn: 3.311 ± 0.44
1.611AlaPro: 1.611 ± 0.337
3.311AlaGln: 3.311 ± 0.588
6.353AlaArg: 6.353 ± 1.204
4.832AlaSer: 4.832 ± 1.054
5.548AlaThr: 5.548 ± 0.889
5.369AlaVal: 5.369 ± 0.633
1.163AlaTrp: 1.163 ± 0.269
2.595AlaTyr: 2.595 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.805CysAla: 0.805 ± 0.235
0.089CysCys: 0.089 ± 0.109
0.805CysAsp: 0.805 ± 0.291
0.984CysGlu: 0.984 ± 0.271
0.268CysPhe: 0.268 ± 0.196
1.342CysGly: 1.342 ± 0.391
0.626CysHis: 0.626 ± 0.252
0.716CysIle: 0.716 ± 0.258
0.358CysLys: 0.358 ± 0.177
1.432CysLeu: 1.432 ± 0.333
0.179CysMet: 0.179 ± 0.131
0.358CysAsn: 0.358 ± 0.172
0.358CysPro: 0.358 ± 0.184
0.358CysGln: 0.358 ± 0.177
1.432CysArg: 1.432 ± 0.408
1.074CysSer: 1.074 ± 0.358
0.447CysThr: 0.447 ± 0.217
0.895CysVal: 0.895 ± 0.327
0.089CysTrp: 0.089 ± 0.1
0.358CysTyr: 0.358 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
4.653AspAla: 4.653 ± 0.667
0.358AspCys: 0.358 ± 0.193
3.937AspAsp: 3.937 ± 0.58
3.937AspGlu: 3.937 ± 0.438
1.611AspPhe: 1.611 ± 0.31
4.742AspGly: 4.742 ± 0.601
1.074AspHis: 1.074 ± 0.343
2.774AspIle: 2.774 ± 0.453
2.684AspLys: 2.684 ± 0.494
4.563AspLeu: 4.563 ± 0.662
0.895AspMet: 0.895 ± 0.322
2.326AspAsn: 2.326 ± 0.474
1.969AspPro: 1.969 ± 0.424
1.163AspGln: 1.163 ± 0.28
2.416AspArg: 2.416 ± 0.454
3.848AspSer: 3.848 ± 0.741
2.774AspThr: 2.774 ± 0.516
4.295AspVal: 4.295 ± 0.824
0.984AspTrp: 0.984 ± 0.252
2.237AspTyr: 2.237 ± 0.439
0.0AspXaa: 0.0 ± 0.0
Glu
6.353GluAla: 6.353 ± 0.918
1.163GluCys: 1.163 ± 0.311
2.237GluAsp: 2.237 ± 0.472
4.384GluGlu: 4.384 ± 0.662
2.953GluPhe: 2.953 ± 0.456
3.132GluGly: 3.132 ± 0.616
1.074GluHis: 1.074 ± 0.286
5.279GluIle: 5.279 ± 0.839
3.669GluLys: 3.669 ± 0.681
7.069GluLeu: 7.069 ± 0.827
1.879GluMet: 1.879 ± 0.416
3.311GluAsn: 3.311 ± 0.589
2.237GluPro: 2.237 ± 0.443
4.026GluGln: 4.026 ± 0.705
4.921GluArg: 4.921 ± 0.566
2.147GluSer: 2.147 ± 0.369
3.937GluThr: 3.937 ± 0.727
3.758GluVal: 3.758 ± 0.508
1.163GluTrp: 1.163 ± 0.283
3.132GluTyr: 3.132 ± 0.584
0.0GluXaa: 0.0 ± 0.0
Phe
2.416PheAla: 2.416 ± 0.408
0.805PheCys: 0.805 ± 0.254
1.342PheAsp: 1.342 ± 0.428
2.416PheGlu: 2.416 ± 0.452
1.611PhePhe: 1.611 ± 0.532
2.684PheGly: 2.684 ± 0.455
0.895PheHis: 0.895 ± 0.27
1.79PheIle: 1.79 ± 0.444
1.7PheLys: 1.7 ± 0.425
3.042PheLeu: 3.042 ± 0.511
1.611PheMet: 1.611 ± 0.378
2.684PheAsn: 2.684 ± 0.499
1.074PhePro: 1.074 ± 0.29
1.163PheGln: 1.163 ± 0.276
2.416PheArg: 2.416 ± 0.519
3.848PheSer: 3.848 ± 0.593
1.253PheThr: 1.253 ± 0.337
2.505PheVal: 2.505 ± 0.398
0.447PheTrp: 0.447 ± 0.204
1.074PheTyr: 1.074 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
5.19GlyAla: 5.19 ± 1.005
0.626GlyCys: 0.626 ± 0.265
3.758GlyAsp: 3.758 ± 0.577
4.742GlyGlu: 4.742 ± 0.629
3.311GlyPhe: 3.311 ± 0.499
4.205GlyGly: 4.205 ± 0.856
0.984GlyHis: 0.984 ± 0.191
3.758GlyIle: 3.758 ± 0.734
4.295GlyLys: 4.295 ± 0.648
6.979GlyLeu: 6.979 ± 0.906
2.237GlyMet: 2.237 ± 0.456
3.49GlyAsn: 3.49 ± 0.617
1.253GlyPro: 1.253 ± 0.32
1.969GlyGln: 1.969 ± 0.332
3.132GlyArg: 3.132 ± 0.586
3.669GlySer: 3.669 ± 0.569
3.49GlyThr: 3.49 ± 0.602
5.011GlyVal: 5.011 ± 0.653
0.984GlyTrp: 0.984 ± 0.258
3.758GlyTyr: 3.758 ± 0.564
0.0GlyXaa: 0.0 ± 0.0
His
1.253HisAla: 1.253 ± 0.419
0.447HisCys: 0.447 ± 0.197
0.537HisAsp: 0.537 ± 0.216
1.074HisGlu: 1.074 ± 0.281
0.537HisPhe: 0.537 ± 0.245
0.805HisGly: 0.805 ± 0.269
0.537HisHis: 0.537 ± 0.184
0.984HisIle: 0.984 ± 0.239
1.79HisLys: 1.79 ± 0.378
1.879HisLeu: 1.879 ± 0.377
0.716HisMet: 0.716 ± 0.195
0.537HisAsn: 0.537 ± 0.221
0.895HisPro: 0.895 ± 0.254
1.074HisGln: 1.074 ± 0.402
1.074HisArg: 1.074 ± 0.282
0.984HisSer: 0.984 ± 0.297
1.074HisThr: 1.074 ± 0.297
1.074HisVal: 1.074 ± 0.3
0.0HisTrp: 0.0 ± 0.0
0.268HisTyr: 0.268 ± 0.128
0.0HisXaa: 0.0 ± 0.0
Ile
5.279IleAla: 5.279 ± 0.567
0.984IleCys: 0.984 ± 0.383
4.205IleAsp: 4.205 ± 0.534
4.116IleGlu: 4.116 ± 0.735
1.79IlePhe: 1.79 ± 0.443
3.4IleGly: 3.4 ± 0.571
0.716IleHis: 0.716 ± 0.242
4.295IleIle: 4.295 ± 0.832
4.653IleLys: 4.653 ± 0.792
3.579IleLeu: 3.579 ± 0.624
1.253IleMet: 1.253 ± 0.312
3.579IleAsn: 3.579 ± 0.517
2.058IlePro: 2.058 ± 0.483
1.879IleGln: 1.879 ± 0.362
4.653IleArg: 4.653 ± 0.558
5.727IleSer: 5.727 ± 0.782
2.774IleThr: 2.774 ± 0.454
2.505IleVal: 2.505 ± 0.49
0.805IleTrp: 0.805 ± 0.276
1.969IleTyr: 1.969 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
5.1LysAla: 5.1 ± 0.679
0.984LysCys: 0.984 ± 0.337
2.595LysAsp: 2.595 ± 0.36
3.311LysGlu: 3.311 ± 0.583
2.953LysPhe: 2.953 ± 0.538
3.758LysGly: 3.758 ± 0.515
0.626LysHis: 0.626 ± 0.225
3.579LysIle: 3.579 ± 0.617
4.205LysLys: 4.205 ± 0.814
4.921LysLeu: 4.921 ± 0.778
1.611LysMet: 1.611 ± 0.345
3.4LysAsn: 3.4 ± 0.655
2.326LysPro: 2.326 ± 0.412
3.042LysGln: 3.042 ± 0.484
3.311LysArg: 3.311 ± 0.603
4.295LysSer: 4.295 ± 0.649
4.116LysThr: 4.116 ± 0.641
3.579LysVal: 3.579 ± 0.535
0.984LysTrp: 0.984 ± 0.295
1.7LysTyr: 1.7 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
7.785LeuAla: 7.785 ± 0.92
1.342LeuCys: 1.342 ± 0.324
4.474LeuAsp: 4.474 ± 0.703
5.816LeuGlu: 5.816 ± 0.739
3.221LeuPhe: 3.221 ± 0.637
4.295LeuGly: 4.295 ± 0.678
1.342LeuHis: 1.342 ± 0.348
6.084LeuIle: 6.084 ± 0.73
6.621LeuLys: 6.621 ± 0.825
6.532LeuLeu: 6.532 ± 0.765
1.879LeuMet: 1.879 ± 0.333
5.1LeuAsn: 5.1 ± 0.66
3.221LeuPro: 3.221 ± 0.552
3.311LeuGln: 3.311 ± 0.486
7.427LeuArg: 7.427 ± 0.876
7.963LeuSer: 7.963 ± 0.665
5.279LeuThr: 5.279 ± 0.627
5.458LeuVal: 5.458 ± 0.561
1.163LeuTrp: 1.163 ± 0.305
1.7LeuTyr: 1.7 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
2.326MetAla: 2.326 ± 0.499
0.089MetCys: 0.089 ± 0.079
1.253MetAsp: 1.253 ± 0.311
1.074MetGlu: 1.074 ± 0.326
0.716MetPhe: 0.716 ± 0.227
1.342MetGly: 1.342 ± 0.308
0.268MetHis: 0.268 ± 0.145
1.969MetIle: 1.969 ± 0.5
2.774MetLys: 2.774 ± 0.495
1.879MetLeu: 1.879 ± 0.346
0.895MetMet: 0.895 ± 0.35
1.611MetAsn: 1.611 ± 0.329
1.253MetPro: 1.253 ± 0.398
1.79MetGln: 1.79 ± 0.373
1.521MetArg: 1.521 ± 0.273
1.879MetSer: 1.879 ± 0.471
1.7MetThr: 1.7 ± 0.382
1.432MetVal: 1.432 ± 0.375
0.268MetTrp: 0.268 ± 0.17
0.716MetTyr: 0.716 ± 0.275
0.0MetXaa: 0.0 ± 0.0
Asn
4.474AsnAla: 4.474 ± 0.877
0.179AsnCys: 0.179 ± 0.134
2.147AsnAsp: 2.147 ± 0.53
3.311AsnGlu: 3.311 ± 0.501
1.7AsnPhe: 1.7 ± 0.396
3.579AsnGly: 3.579 ± 0.595
1.521AsnHis: 1.521 ± 0.311
3.042AsnIle: 3.042 ± 0.39
2.326AsnLys: 2.326 ± 0.419
3.848AsnLeu: 3.848 ± 0.556
1.7AsnMet: 1.7 ± 0.359
2.505AsnAsn: 2.505 ± 0.465
1.432AsnPro: 1.432 ± 0.313
1.611AsnGln: 1.611 ± 0.381
2.237AsnArg: 2.237 ± 0.573
3.49AsnSer: 3.49 ± 0.511
2.595AsnThr: 2.595 ± 0.545
2.774AsnVal: 2.774 ± 0.398
0.984AsnTrp: 0.984 ± 0.232
1.969AsnTyr: 1.969 ± 0.376
0.0AsnXaa: 0.0 ± 0.0
Pro
2.416ProAla: 2.416 ± 0.477
0.447ProCys: 0.447 ± 0.193
2.863ProAsp: 2.863 ± 0.582
3.132ProGlu: 3.132 ± 0.48
1.342ProPhe: 1.342 ± 0.325
1.7ProGly: 1.7 ± 0.323
0.984ProHis: 0.984 ± 0.313
0.984ProIle: 0.984 ± 0.295
1.342ProLys: 1.342 ± 0.352
3.4ProLeu: 3.4 ± 0.502
0.626ProMet: 0.626 ± 0.253
1.074ProAsn: 1.074 ± 0.321
1.074ProPro: 1.074 ± 0.329
1.253ProGln: 1.253 ± 0.258
1.253ProArg: 1.253 ± 0.286
2.505ProSer: 2.505 ± 0.416
1.879ProThr: 1.879 ± 0.51
2.953ProVal: 2.953 ± 0.605
0.447ProTrp: 0.447 ± 0.196
1.074ProTyr: 1.074 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
3.937GlnAla: 3.937 ± 0.91
0.716GlnCys: 0.716 ± 0.248
1.432GlnAsp: 1.432 ± 0.379
2.863GlnGlu: 2.863 ± 0.531
0.895GlnPhe: 0.895 ± 0.203
2.416GlnGly: 2.416 ± 0.499
1.074GlnHis: 1.074 ± 0.268
2.505GlnIle: 2.505 ± 0.456
2.863GlnLys: 2.863 ± 0.465
2.774GlnLeu: 2.774 ± 0.468
1.521GlnMet: 1.521 ± 0.308
2.684GlnAsn: 2.684 ± 0.561
0.805GlnPro: 0.805 ± 0.27
2.058GlnGln: 2.058 ± 0.52
4.205GlnArg: 4.205 ± 0.678
3.221GlnSer: 3.221 ± 0.715
1.969GlnThr: 1.969 ± 0.304
1.879GlnVal: 1.879 ± 0.434
0.805GlnTrp: 0.805 ± 0.32
1.074GlnTyr: 1.074 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
5.906ArgAla: 5.906 ± 0.907
0.716ArgCys: 0.716 ± 0.324
3.579ArgAsp: 3.579 ± 0.614
4.921ArgGlu: 4.921 ± 0.712
2.595ArgPhe: 2.595 ± 0.508
4.742ArgGly: 4.742 ± 0.775
1.253ArgHis: 1.253 ± 0.357
3.579ArgIle: 3.579 ± 0.688
4.116ArgLys: 4.116 ± 0.611
6.174ArgLeu: 6.174 ± 0.714
1.521ArgMet: 1.521 ± 0.376
2.505ArgAsn: 2.505 ± 0.424
1.521ArgPro: 1.521 ± 0.363
3.042ArgGln: 3.042 ± 0.726
3.132ArgArg: 3.132 ± 0.545
3.758ArgSer: 3.758 ± 0.518
4.026ArgThr: 4.026 ± 0.52
2.863ArgVal: 2.863 ± 0.519
1.253ArgTrp: 1.253 ± 0.318
1.79ArgTyr: 1.79 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
6.8SerAla: 6.8 ± 1.162
0.895SerCys: 0.895 ± 0.28
4.474SerAsp: 4.474 ± 0.501
4.295SerGlu: 4.295 ± 0.558
1.79SerPhe: 1.79 ± 0.331
6.8SerGly: 6.8 ± 0.754
0.716SerHis: 0.716 ± 0.286
3.042SerIle: 3.042 ± 0.604
4.384SerLys: 4.384 ± 0.639
7.248SerLeu: 7.248 ± 1.182
2.237SerMet: 2.237 ± 0.449
2.953SerAsn: 2.953 ± 0.447
2.505SerPro: 2.505 ± 0.447
3.311SerGln: 3.311 ± 0.482
4.295SerArg: 4.295 ± 0.67
4.832SerSer: 4.832 ± 0.762
3.311SerThr: 3.311 ± 0.433
3.937SerVal: 3.937 ± 0.769
0.626SerTrp: 0.626 ± 0.24
1.7SerTyr: 1.7 ± 0.491
0.0SerXaa: 0.0 ± 0.0
Thr
5.369ThrAla: 5.369 ± 0.877
0.626ThrCys: 0.626 ± 0.236
3.311ThrAsp: 3.311 ± 0.505
4.205ThrGlu: 4.205 ± 0.544
2.058ThrPhe: 2.058 ± 0.476
4.653ThrGly: 4.653 ± 0.619
0.358ThrHis: 0.358 ± 0.167
4.116ThrIle: 4.116 ± 0.925
2.774ThrLys: 2.774 ± 0.497
5.727ThrLeu: 5.727 ± 0.67
0.984ThrMet: 0.984 ± 0.289
1.7ThrAsn: 1.7 ± 0.37
2.326ThrPro: 2.326 ± 0.477
2.147ThrGln: 2.147 ± 0.432
2.953ThrArg: 2.953 ± 0.433
3.758ThrSer: 3.758 ± 0.647
3.221ThrThr: 3.221 ± 0.468
3.758ThrVal: 3.758 ± 0.626
0.716ThrTrp: 0.716 ± 0.289
1.521ThrTyr: 1.521 ± 0.344
0.0ThrXaa: 0.0 ± 0.0
Val
4.295ValAla: 4.295 ± 0.612
1.074ValCys: 1.074 ± 0.342
4.026ValAsp: 4.026 ± 0.617
3.311ValGlu: 3.311 ± 0.627
2.326ValPhe: 2.326 ± 0.475
3.579ValGly: 3.579 ± 0.611
0.626ValHis: 0.626 ± 0.235
4.026ValIle: 4.026 ± 0.607
3.4ValLys: 3.4 ± 0.602
5.995ValLeu: 5.995 ± 0.562
1.7ValMet: 1.7 ± 0.38
2.953ValAsn: 2.953 ± 0.481
2.953ValPro: 2.953 ± 0.481
2.058ValGln: 2.058 ± 0.464
3.579ValArg: 3.579 ± 0.553
4.295ValSer: 4.295 ± 0.62
3.937ValThr: 3.937 ± 0.649
4.295ValVal: 4.295 ± 0.636
0.805ValTrp: 0.805 ± 0.351
2.237ValTyr: 2.237 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
1.163TrpAla: 1.163 ± 0.243
0.358TrpCys: 0.358 ± 0.185
0.358TrpAsp: 0.358 ± 0.206
1.432TrpGlu: 1.432 ± 0.36
0.537TrpPhe: 0.537 ± 0.217
0.626TrpGly: 0.626 ± 0.21
0.179TrpHis: 0.179 ± 0.122
0.805TrpIle: 0.805 ± 0.249
0.805TrpLys: 0.805 ± 0.205
1.521TrpLeu: 1.521 ± 0.33
0.358TrpMet: 0.358 ± 0.208
0.537TrpAsn: 0.537 ± 0.196
0.358TrpPro: 0.358 ± 0.213
0.895TrpGln: 0.895 ± 0.283
0.805TrpArg: 0.805 ± 0.234
1.342TrpSer: 1.342 ± 0.376
0.895TrpThr: 0.895 ± 0.328
1.163TrpVal: 1.163 ± 0.328
0.268TrpTrp: 0.268 ± 0.147
0.537TrpTyr: 0.537 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.221TyrAla: 3.221 ± 0.434
0.268TyrCys: 0.268 ± 0.157
1.342TyrAsp: 1.342 ± 0.353
1.521TyrGlu: 1.521 ± 0.265
1.253TyrPhe: 1.253 ± 0.258
2.147TyrGly: 2.147 ± 0.373
0.805TyrHis: 0.805 ± 0.304
1.879TyrIle: 1.879 ± 0.373
1.253TyrLys: 1.253 ± 0.304
2.953TyrLeu: 2.953 ± 0.548
0.626TyrMet: 0.626 ± 0.199
0.805TyrAsn: 0.805 ± 0.262
1.521TyrPro: 1.521 ± 0.423
2.237TyrGln: 2.237 ± 0.454
1.879TyrArg: 1.879 ± 0.456
2.684TyrSer: 2.684 ± 0.516
2.058TyrThr: 2.058 ± 0.402
1.969TyrVal: 1.969 ± 0.395
0.984TyrTrp: 0.984 ± 0.311
0.805TyrTyr: 0.805 ± 0.239
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (11177 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski