Dechloromonas sp. HYN0024
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B7A1C3|A0A3B7A1C3_9RHOO Adenine DNA glycosylase OS=Dechloromonas sp. HYN0024 OX=2231055 GN=mutY PE=3 SV=1
MM1 pKa = 7.11 QSWSGNDD8 pKa = 3.14 IIYY11 pKa = 10.62 GNSGDD16 pKa = 4.61 DD17 pKa = 3.63 VLKK20 pKa = 11.12 GGAGDD25 pKa = 4.53 DD26 pKa = 4.37 YY27 pKa = 11.39 IDD29 pKa = 4.35 GGLGIDD35 pKa = 3.51 QSVYY39 pKa = 10.22 SANSSSVSLVRR50 pKa = 11.84 HH51 pKa = 5.91 LKK53 pKa = 10.54 AGGVLLEE60 pKa = 4.28 SAEE63 pKa = 4.35 GSDD66 pKa = 3.1 TLVNVEE72 pKa = 3.88 QVQFNDD78 pKa = 3.48 GTFNLEE84 pKa = 3.73 SFIRR88 pKa = 11.84 SIPTPSYY95 pKa = 8.33 TTDD98 pKa = 3.42 AGNATPTVYY107 pKa = 10.49 SGAVGFLEE115 pKa = 4.36 FQFLGGAVGEE125 pKa = 4.12 IVIGSKK131 pKa = 11.17 YY132 pKa = 10.61 NDD134 pKa = 4.68 FINLLAGDD142 pKa = 4.58 DD143 pKa = 4.04 AADD146 pKa = 3.89 GAGGDD151 pKa = 4.15 DD152 pKa = 4.13 VLDD155 pKa = 4.17 GGIGSNFLTGGAGKK169 pKa = 8.6 DD170 pKa = 3.28 TFFLDD175 pKa = 3.59 GRR177 pKa = 11.84 GGSVTWSTITDD188 pKa = 4.2 FEE190 pKa = 6.53 AGDD193 pKa = 3.77 QVNIWGWQPNVSKK206 pKa = 11.06 LLLTQASAGASGFSGATYY224 pKa = 9.88 HH225 pKa = 7.2 YY226 pKa = 10.48 DD227 pKa = 3.49 LNNDD231 pKa = 3.19 GSIDD235 pKa = 3.65 TSITFAVLALSAVPSPVPSDD255 pKa = 3.59 VAGNGYY261 pKa = 9.99 LLFGG265 pKa = 4.48
Molecular weight: 27.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.605
IPC_protein 3.63
Toseland 3.389
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.63
Rodwell 3.452
Grimsley 3.3
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 4.062
Thurlkill 3.465
EMBOSS 3.63
Sillero 3.757
Patrickios 1.863
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A3B7A917|A0A3B7A917_9RHOO P-II family nitrogen regulator OS=Dechloromonas sp. HYN0024 OX=2231055 GN=HYN24_01465 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.72 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.53 GGRR28 pKa = 11.84 AVIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.38 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LAVV44 pKa = 3.19
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3011
0
3011
978272
37
3669
324.9
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.514 ± 0.056
1.052 ± 0.019
5.445 ± 0.033
5.791 ± 0.042
3.738 ± 0.027
8.064 ± 0.049
2.208 ± 0.022
5.428 ± 0.036
4.11 ± 0.042
10.681 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.562 ± 0.024
3.118 ± 0.031
4.808 ± 0.035
3.723 ± 0.03
6.223 ± 0.048
5.541 ± 0.04
5.146 ± 0.062
7.211 ± 0.049
1.31 ± 0.02
2.327 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here