Bacilladnaviridae sp.
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MP94|A0A345MP94_9VIRU Uncharacterized protein OS=Bacilladnaviridae sp. OX=2202647 PE=4 SV=1
MM1 pKa = 7.5 SIKK4 pKa = 10.39 PIMRR8 pKa = 11.84 SVSVPTVLPDD18 pKa = 3.12 SQMYY22 pKa = 9.6 FLHH25 pKa = 6.97 RR26 pKa = 11.84 NAGTEE31 pKa = 3.78 LLDD34 pKa = 3.38 YY35 pKa = 11.05 HH36 pKa = 7.16 FDD38 pKa = 3.65 VLSGLIYY45 pKa = 10.83 LNARR49 pKa = 11.84 GHH51 pKa = 5.39 RR52 pKa = 11.84 TVHH55 pKa = 5.41 YY56 pKa = 10.8 VRR58 pKa = 11.84 DD59 pKa = 4.18 LIKK62 pKa = 10.91 AIDD65 pKa = 3.77 DD66 pKa = 3.13 WHH68 pKa = 6.38 YY69 pKa = 10.94 RR70 pKa = 11.84 ALVDD74 pKa = 5.19 DD75 pKa = 4.54 RR76 pKa = 11.84 EE77 pKa = 4.26 KK78 pKa = 11.24 EE79 pKa = 3.91 QLQQLILVDD88 pKa = 4.24 LMTNEE93 pKa = 4.48 EE94 pKa = 4.48 LYY96 pKa = 10.84 KK97 pKa = 10.92 DD98 pKa = 3.56 AVIWDD103 pKa = 4.04 GTRR106 pKa = 11.84 KK107 pKa = 9.71 RR108 pKa = 11.84 YY109 pKa = 9.68 YY110 pKa = 10.9 VIDD113 pKa = 3.03 AWIRR117 pKa = 11.84 NHH119 pKa = 5.1 STCFEE124 pKa = 4.03 FTGPIDD130 pKa = 3.57 PANIKK135 pKa = 10.23 VEE137 pKa = 4.23 EE138 pKa = 4.26 EE139 pKa = 4.17 EE140 pKa = 4.42 PTNLDD145 pKa = 3.34 DD146 pKa = 4.08 TFSLGTITTFTMDD159 pKa = 4.13 EE160 pKa = 4.42 SPLGLVGGAIEE171 pKa = 5.13 FDD173 pKa = 3.38 WEE175 pKa = 4.24 LDD177 pKa = 3.73 SNGYY181 pKa = 9.32 LLNDD185 pKa = 3.78 FVVTVDD191 pKa = 4.8 DD192 pKa = 4.31 PLII195 pKa = 4.45
Molecular weight: 22.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.384
IPC2_protein 4.38
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.329
Bjellqvist 4.469
Wikipedia 4.24
Rodwell 4.19
Grimsley 4.075
Solomon 4.317
Lehninger 4.279
Nozaki 4.431
DTASelect 4.66
Thurlkill 4.19
EMBOSS 4.253
Sillero 4.469
Patrickios 3.592
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.384
Protein with the highest isoelectric point:
>tr|A0A345MP95|A0A345MP95_9VIRU Putative esterase/lipase OS=Bacilladnaviridae sp. OX=2202647 PE=4 SV=1
MM1 pKa = 7.43 VKK3 pKa = 10.19 RR4 pKa = 11.84 KK5 pKa = 8.58 RR6 pKa = 11.84 TIGGPRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 8.67 STRR17 pKa = 11.84 QTKK20 pKa = 9.97 KK21 pKa = 7.55 RR22 pKa = 11.84 TKK24 pKa = 7.3 WTSRR28 pKa = 11.84 SYY30 pKa = 11.01 TPAPKK35 pKa = 9.62 PKK37 pKa = 9.48 KK38 pKa = 9.87 RR39 pKa = 11.84 KK40 pKa = 9.11 ASKK43 pKa = 9.47 PRR45 pKa = 11.84 YY46 pKa = 8.31 AAQHH50 pKa = 5.3 HH51 pKa = 7.3 AYY53 pKa = 8.87 MKK55 pKa = 10.98 SPFSKK60 pKa = 10.54 NASKK64 pKa = 10.53 QPKK67 pKa = 9.23 IPDD70 pKa = 3.6 GAFTTSMGRR79 pKa = 11.84 AFEE82 pKa = 4.53 TIAEE86 pKa = 4.19 IQNAVGNEE94 pKa = 4.12 TIDD97 pKa = 3.1 IVLFPGFGIGAAIQNTTAAATMDD120 pKa = 4.04 GVQPIALDD128 pKa = 4.27 HH129 pKa = 5.47 STNWLGSADD138 pKa = 3.6 TSKK141 pKa = 11.26 SVDD144 pKa = 3.55 NEE146 pKa = 4.15 SEE148 pKa = 3.87 IARR151 pKa = 11.84 WRR153 pKa = 11.84 LVAQSLQLLLVNSAEE168 pKa = 4.47 EE169 pKa = 3.91 NNGWYY174 pKa = 6.47 EE175 pKa = 4.14 TCRR178 pKa = 11.84 FTPAQFMADD187 pKa = 3.27 YY188 pKa = 10.59 VVRR191 pKa = 11.84 NTDD194 pKa = 2.95 GNNIEE199 pKa = 4.45 NLTNAALCPHH209 pKa = 6.47 NKK211 pKa = 9.47 FFTDD215 pKa = 4.6 HH216 pKa = 5.75 ITDD219 pKa = 3.27 MVMTNQRR226 pKa = 11.84 GYY228 pKa = 11.22 CLGKK232 pKa = 9.12 LTDD235 pKa = 3.85 LGKK238 pKa = 10.58 KK239 pKa = 9.55 EE240 pKa = 4.06 FLLHH244 pKa = 6.57 NISPKK249 pKa = 10.52 DD250 pKa = 3.47 DD251 pKa = 4.88 GIINMQEE258 pKa = 3.9 TYY260 pKa = 11.23 NNSFANGTYY269 pKa = 10.37 DD270 pKa = 5.51 AINKK274 pKa = 7.44 LHH276 pKa = 6.55 SWTPDD281 pKa = 2.54 HH282 pKa = 7.45 DD283 pKa = 4.16 AQIMMRR289 pKa = 11.84 LLDD292 pKa = 3.76 QGTSLNSHH300 pKa = 6.36 DD301 pKa = 4.41 MILIRR306 pKa = 11.84 LHH308 pKa = 6.33 CRR310 pKa = 11.84 SNTGTSPNTGSKK322 pKa = 10.21 FVLRR326 pKa = 11.84 LGAGHH331 pKa = 5.76 EE332 pKa = 4.22 VEE334 pKa = 4.61 YY335 pKa = 10.4 TPKK338 pKa = 10.68 SPLHH342 pKa = 5.56 TFMTEE347 pKa = 3.66 SPTVTGTNKK356 pKa = 10.46 LKK358 pKa = 10.79 DD359 pKa = 3.74 DD360 pKa = 4.77 ANNKK364 pKa = 8.67 PDD366 pKa = 4.04 AVTSAPFTLGGGG378 pKa = 3.64
Molecular weight: 41.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.839
IPC2_protein 8.697
IPC_protein 8.595
Toseland 9.443
ProMoST 9.151
Dawson 9.692
Bjellqvist 9.385
Wikipedia 9.823
Rodwell 10.058
Grimsley 9.736
Solomon 9.721
Lehninger 9.692
Nozaki 9.56
DTASelect 9.341
Thurlkill 9.545
EMBOSS 9.867
Sillero 9.633
Patrickios 5.436
IPC_peptide 9.721
IPC2_peptide 7.951
IPC2.peptide.svr19 7.843
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1293
195
440
323.3
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.347 ± 0.871
0.773 ± 0.231
7.27 ± 0.849
4.872 ± 0.501
3.79 ± 0.444
6.883 ± 0.656
3.79 ± 0.393
4.872 ± 0.485
6.265 ± 0.754
7.193 ± 0.928
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.398 ± 0.349
5.027 ± 0.926
4.95 ± 0.671
3.48 ± 0.47
5.336 ± 0.15
6.574 ± 0.386
7.502 ± 0.978
6.342 ± 1.024
2.243 ± 0.409
3.094 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here