Streptococcus satellite phage Javan468
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPN4|A0A4D5ZPN4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan468 OX=2558716 GN=JavanS468_0015 PE=4 SV=1
MM1 pKa = 7.53 NDD3 pKa = 4.12 KK4 pKa = 10.55 ILSNYY9 pKa = 9.55 EE10 pKa = 3.95 LLCTEE15 pKa = 4.85 LEE17 pKa = 4.34 NVISTLEE24 pKa = 3.85 MAITDD29 pKa = 3.55 IDD31 pKa = 3.72 QDD33 pKa = 3.48 KK34 pKa = 9.69 ATALVAVATNGLKK47 pKa = 10.58 HH48 pKa = 6.21 LVSEE52 pKa = 4.35 HH53 pKa = 6.38 TEE55 pKa = 3.65 LSDD58 pKa = 4.95 RR59 pKa = 11.84 FFKK62 pKa = 10.73 EE63 pKa = 3.6 YY64 pKa = 10.97 DD65 pKa = 3.3 NEE67 pKa = 4.15
Molecular weight: 7.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.345
IPC2_protein 4.317
IPC_protein 4.177
Toseland 4.012
ProMoST 4.24
Dawson 4.139
Bjellqvist 4.368
Wikipedia 4.037
Rodwell 4.024
Grimsley 3.935
Solomon 4.126
Lehninger 4.088
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.05
EMBOSS 4.05
Sillero 4.291
Patrickios 3.592
IPC_peptide 4.139
IPC2_peptide 4.279
IPC2.peptide.svr19 4.216
Protein with the highest isoelectric point:
>tr|A0A4D5ZR12|A0A4D5ZR12_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan468 OX=2558716 GN=JavanS468_0013 PE=4 SV=1
MM1 pKa = 7.82 LITEE5 pKa = 5.39 GIAVKK10 pKa = 10.13 VRR12 pKa = 11.84 KK13 pKa = 9.55 KK14 pKa = 9.97 RR15 pKa = 11.84 TVEE18 pKa = 3.81 RR19 pKa = 11.84 LGKK22 pKa = 9.97 VALAKK27 pKa = 10.56 KK28 pKa = 10.42 LGVTPPTLGKK38 pKa = 10.3 VEE40 pKa = 4.23 NGNYY44 pKa = 8.24 DD45 pKa = 3.37 APKK48 pKa = 10.04 RR49 pKa = 11.84 IYY51 pKa = 10.18 EE52 pKa = 4.36 SVMTWLVEE60 pKa = 4.49 EE61 pKa = 4.4 II62 pKa = 4.06
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.976
IPC2_protein 9.18
IPC_protein 9.151
Toseland 10.101
ProMoST 9.633
Dawson 10.233
Bjellqvist 9.823
Wikipedia 10.35
Rodwell 10.862
Grimsley 10.277
Solomon 10.262
Lehninger 10.248
Nozaki 10.058
DTASelect 9.823
Thurlkill 10.101
EMBOSS 10.482
Sillero 10.131
Patrickios 10.672
IPC_peptide 10.277
IPC2_peptide 8.2
IPC2.peptide.svr19 8.224
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2805
56
396
147.6
17.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.526 ± 0.499
0.606 ± 0.137
5.954 ± 0.354
8.77 ± 0.998
3.529 ± 0.418
4.635 ± 0.346
1.711 ± 0.21
7.736 ± 0.729
9.875 ± 0.608
9.697 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.137 ± 0.245
6.061 ± 0.545
2.531 ± 0.241
4.563 ± 0.559
3.636 ± 0.382
5.455 ± 0.495
5.49 ± 0.514
6.096 ± 0.53
1.07 ± 0.211
3.922 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here