Mesonia sp. HuA40

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mesonia; unclassified Mesonia

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2381 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8L413|A0A5C8L413_9FLAO DUF3810 domain-containing protein OS=Mesonia sp. HuA40 OX=2602761 GN=FT993_10855 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.33KK3 pKa = 9.43ILPFILCNLLALASFAQIGINTDD26 pKa = 3.41SPSPAAALEE35 pKa = 4.33IKK37 pKa = 10.49SSYY40 pKa = 9.4NNKK43 pKa = 10.2NYY45 pKa = 10.69GGLLIPTVDD54 pKa = 2.82ATEE57 pKa = 4.02RR58 pKa = 11.84DD59 pKa = 4.02KK60 pKa = 11.4ISDD63 pKa = 3.58KK64 pKa = 10.92ATTADD69 pKa = 3.53GGMLIYY75 pKa = 10.42YY76 pKa = 9.85VEE78 pKa = 4.53GNTRR82 pKa = 11.84CLQIYY87 pKa = 10.28DD88 pKa = 4.95GINGSWADD96 pKa = 4.03VYY98 pKa = 11.46CMPTNYY104 pKa = 10.42APIASNVFISGIIEE118 pKa = 3.99DD119 pKa = 4.35TQILTANYY127 pKa = 7.87TYY129 pKa = 11.45SDD131 pKa = 3.94IEE133 pKa = 4.06NDD135 pKa = 3.7AEE137 pKa = 4.4GTTTFEE143 pKa = 3.97WFIADD148 pKa = 4.2DD149 pKa = 4.02AQGTNTTSLGVTTTGTYY166 pKa = 9.04TLQTADD172 pKa = 3.02VGKK175 pKa = 10.42FIFVRR180 pKa = 11.84VTPVASAGEE189 pKa = 4.54FIGSPVDD196 pKa = 3.48SPLRR200 pKa = 11.84GPIEE204 pKa = 4.02AAGGVASDD212 pKa = 4.37LFISEE217 pKa = 4.44YY218 pKa = 11.2VEE220 pKa = 3.96GSSYY224 pKa = 11.62NKK226 pKa = 9.94IIEE229 pKa = 4.18IANFTGQDD237 pKa = 3.47VNLSEE242 pKa = 3.87YY243 pKa = 9.94HH244 pKa = 6.49LRR246 pKa = 11.84IFRR249 pKa = 11.84QTGNLTGPISLTSNTLIDD267 pKa = 3.7GDD269 pKa = 4.12VFVIANTANVLATGEE284 pKa = 4.33VDD286 pKa = 3.49TQTGSLNFNGDD297 pKa = 3.78DD298 pKa = 4.07SVLLYY303 pKa = 8.39YY304 pKa = 10.49QPLGGTEE311 pKa = 4.02TLIDD315 pKa = 4.01IIGPDD320 pKa = 3.59SLVNPPSNNPNFAKK334 pKa = 10.72DD335 pKa = 3.12EE336 pKa = 4.17TLRR339 pKa = 11.84RR340 pKa = 11.84KK341 pKa = 9.86PGLGPSTTYY350 pKa = 11.05DD351 pKa = 3.45SNDD354 pKa = 3.18FDD356 pKa = 4.22SFSIDD361 pKa = 3.32EE362 pKa = 4.16VSDD365 pKa = 3.66IGSHH369 pKa = 5.96TYY371 pKa = 10.48

Molecular weight: 39.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8L7H1|A0A5C8L7H1_9FLAO Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Mesonia sp. HuA40 OX=2602761 GN=FT993_11195 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2381

0

2381

784139

32

2408

329.3

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.843 ± 0.054

0.718 ± 0.017

5.212 ± 0.038

6.676 ± 0.054

5.225 ± 0.04

6.048 ± 0.054

1.84 ± 0.023

7.559 ± 0.048

7.962 ± 0.074

9.967 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.024

6.078 ± 0.055

3.486 ± 0.025

4.417 ± 0.037

3.554 ± 0.035

6.041 ± 0.037

5.293 ± 0.05

5.931 ± 0.048

1.012 ± 0.017

4.09 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski