Torque teno virus (isolate Human/China/CT39F/2001) (TTV)
Average proteome isoelectric point is 8.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8V7G3|CAPSD_TTVV7 Capsid protein OS=Torque teno virus (isolate Human/China/CT39F/2001) OX=486279 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.37 SIWRR5 pKa = 11.84 PPLHH9 pKa = 6.15 NVPGLEE15 pKa = 4.08 HH16 pKa = 6.59 LWYY19 pKa = 9.8 EE20 pKa = 4.45 SVHH23 pKa = 6.19 RR24 pKa = 11.84 SHH26 pKa = 7.16 AAVCGCGDD34 pKa = 3.83 PVRR37 pKa = 11.84 HH38 pKa = 5.3 LTALAEE44 pKa = 4.4 RR45 pKa = 11.84 YY46 pKa = 8.75 GIPGGSRR53 pKa = 11.84 SSGAPGVGGNHH64 pKa = 6.24 NPPQIRR70 pKa = 11.84 RR71 pKa = 11.84 ARR73 pKa = 11.84 HH74 pKa = 5.02 PAAAPDD80 pKa = 4.21 PPAGNQPPALPWHH93 pKa = 6.68 GDD95 pKa = 3.07 GGNEE99 pKa = 3.89 SGAGGGEE106 pKa = 4.08 SGGPVADD113 pKa = 4.54 FADD116 pKa = 5.0 DD117 pKa = 4.2 GLDD120 pKa = 3.75 DD121 pKa = 5.35 LVAALDD127 pKa = 3.81 EE128 pKa = 4.67 EE129 pKa = 4.8 EE130 pKa = 4.1
Molecular weight: 13.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.02
IPC2_protein 5.029
IPC_protein 4.965
Toseland 5.067
ProMoST 5.041
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.978
Rodwell 4.978
Grimsley 5.054
Solomon 5.054
Lehninger 5.016
Nozaki 5.219
DTASelect 5.385
Thurlkill 5.118
EMBOSS 5.092
Sillero 5.283
Patrickios 3.783
IPC_peptide 5.054
IPC2_peptide 5.296
IPC2.peptide.svr19 5.115
Protein with the highest isoelectric point:
>sp|Q8V7G4|ORF23_TTVV7 Probable protein VP2 OS=Torque teno virus (isolate Human/China/CT39F/2001) OX=486279 GN=ORF2/3 PE=3 SV=1
MM1 pKa = 7.53 AWGWWKK7 pKa = 10.56 RR8 pKa = 11.84 KK9 pKa = 9.12 RR10 pKa = 11.84 RR11 pKa = 11.84 WWWRR15 pKa = 11.84 KK16 pKa = 6.64 RR17 pKa = 11.84 WTRR20 pKa = 11.84 GRR22 pKa = 11.84 LRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 WPRR29 pKa = 11.84 RR30 pKa = 11.84 SRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 PRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 VRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 WRR47 pKa = 11.84 RR48 pKa = 11.84 GRR50 pKa = 11.84 PRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 LYY56 pKa = 10.55 RR57 pKa = 11.84 RR58 pKa = 11.84 GRR60 pKa = 11.84 RR61 pKa = 11.84 YY62 pKa = 9.06 RR63 pKa = 11.84 RR64 pKa = 11.84 KK65 pKa = 9.81 RR66 pKa = 11.84 KK67 pKa = 8.89 RR68 pKa = 11.84 AKK70 pKa = 8.53 ITIRR74 pKa = 11.84 QWQPAMTRR82 pKa = 11.84 RR83 pKa = 11.84 CFIRR87 pKa = 11.84 GHH89 pKa = 5.67 MPALICGWGAYY100 pKa = 9.15 ASNYY104 pKa = 7.97 TSHH107 pKa = 8.24 LEE109 pKa = 3.99 DD110 pKa = 5.44 KK111 pKa = 10.37 IVKK114 pKa = 9.09 GPYY117 pKa = 9.69 GGGHH121 pKa = 4.82 ATFRR125 pKa = 11.84 FSLQVLCEE133 pKa = 4.03 EE134 pKa = 4.64 HH135 pKa = 6.8 LKK137 pKa = 9.29 HH138 pKa = 6.17 HH139 pKa = 6.45 NYY141 pKa = 6.52 WTRR144 pKa = 11.84 SNQDD148 pKa = 3.17 LEE150 pKa = 4.06 LALYY154 pKa = 10.05 YY155 pKa = 10.81 GATIKK160 pKa = 10.52 FYY162 pKa = 10.74 RR163 pKa = 11.84 SPDD166 pKa = 2.79 TDD168 pKa = 4.27 FIVTYY173 pKa = 8.18 QRR175 pKa = 11.84 KK176 pKa = 9.02 SPLGGNILTAPSLHH190 pKa = 6.78 PAEE193 pKa = 5.48 AMLSKK198 pKa = 10.91 NKK200 pKa = 10.0 ILIPSLQTKK209 pKa = 9.86 PKK211 pKa = 9.63 GKK213 pKa = 8.71 KK214 pKa = 5.73 TVKK217 pKa = 10.49 VNIPPPTLFVHH228 pKa = 6.37 KK229 pKa = 9.77 WYY231 pKa = 9.46 FQKK234 pKa = 10.69 DD235 pKa = 3.31 ICDD238 pKa = 3.36 LTLFNLNVVAADD250 pKa = 3.82 LRR252 pKa = 11.84 FPFCSPQTDD261 pKa = 3.61 NVCITFQVLAAEE273 pKa = 4.48 YY274 pKa = 11.05 NNFLSTTLGTTNEE287 pKa = 4.1 STFIEE292 pKa = 4.42 NFLKK296 pKa = 10.97 VAFPDD301 pKa = 3.75 DD302 pKa = 4.01 KK303 pKa = 10.79 PRR305 pKa = 11.84 HH306 pKa = 5.42 SNILNTFRR314 pKa = 11.84 TEE316 pKa = 3.72 GCMSHH321 pKa = 7.04 PQLQKK326 pKa = 10.72 FKK328 pKa = 10.44 PPNTGPGEE336 pKa = 3.96 NKK338 pKa = 10.33 YY339 pKa = 10.81 FFTPDD344 pKa = 3.97 GLWGDD349 pKa = 4.53 PIYY352 pKa = 10.43 IYY354 pKa = 11.29 NNGVQQQTAQQIRR367 pKa = 11.84 EE368 pKa = 4.13 KK369 pKa = 10.45 IKK371 pKa = 10.85 KK372 pKa = 9.99 NMEE375 pKa = 3.52 NYY377 pKa = 7.98 YY378 pKa = 11.21 AKK380 pKa = 10.05 IVEE383 pKa = 4.56 EE384 pKa = 3.91 NTIITKK390 pKa = 10.18 GSKK393 pKa = 9.16 AHH395 pKa = 6.16 CHH397 pKa = 4.42 LTGIFSPPFLNIGRR411 pKa = 11.84 VARR414 pKa = 11.84 EE415 pKa = 3.74 FPGLYY420 pKa = 9.52 TDD422 pKa = 4.08 VVYY425 pKa = 11.08 NPWTDD430 pKa = 2.83 KK431 pKa = 11.53 GKK433 pKa = 11.09 GNKK436 pKa = 8.59 IWLDD440 pKa = 3.62 SLTKK444 pKa = 10.23 SDD446 pKa = 4.43 NIYY449 pKa = 10.79 DD450 pKa = 3.78 PRR452 pKa = 11.84 QSILLMADD460 pKa = 3.06 MPLYY464 pKa = 10.66 IMLNGYY470 pKa = 9.17 IDD472 pKa = 3.43 WAKK475 pKa = 10.79 KK476 pKa = 9.0 EE477 pKa = 4.07 RR478 pKa = 11.84 NNWGLATQYY487 pKa = 11.09 RR488 pKa = 11.84 LLLTCPYY495 pKa = 8.81 TFPRR499 pKa = 11.84 LYY501 pKa = 11.12 VEE503 pKa = 4.25 TNPNYY508 pKa = 10.67 GYY510 pKa = 10.92 VPYY513 pKa = 10.36 SEE515 pKa = 5.27 SFGAGQMPDD524 pKa = 3.23 KK525 pKa = 10.85 NPYY528 pKa = 9.6 VPITWRR534 pKa = 11.84 GKK536 pKa = 7.79 WYY538 pKa = 9.28 PHH540 pKa = 6.91 ILHH543 pKa = 6.19 QEE545 pKa = 3.8 AVINDD550 pKa = 3.61 IVISGPFTPKK560 pKa = 9.12 DD561 pKa = 3.77 TKK563 pKa = 10.84 PVMQLNMKK571 pKa = 10.09 YY572 pKa = 10.27 SFRR575 pKa = 11.84 FTWGGNPISTQIVKK589 pKa = 10.31 DD590 pKa = 3.65 PCTQPTFEE598 pKa = 4.86 IPGGGNIPRR607 pKa = 11.84 RR608 pKa = 11.84 IQVINPKK615 pKa = 10.03 VLGPSYY621 pKa = 10.85 SFRR624 pKa = 11.84 SFDD627 pKa = 3.44 LRR629 pKa = 11.84 RR630 pKa = 11.84 DD631 pKa = 3.59 MFSGSSLKK639 pKa = 10.31 RR640 pKa = 11.84 VSEE643 pKa = 3.99 QQEE646 pKa = 3.92 TSEE649 pKa = 4.4 FLFSGGKK656 pKa = 9.25 RR657 pKa = 11.84 PRR659 pKa = 11.84 IDD661 pKa = 3.2 LPKK664 pKa = 10.0 YY665 pKa = 9.87 VPPEE669 pKa = 3.61 EE670 pKa = 4.76 DD671 pKa = 3.37 FNIQEE676 pKa = 4.12 RR677 pKa = 11.84 QQRR680 pKa = 11.84 EE681 pKa = 3.83 QRR683 pKa = 11.84 PWTSEE688 pKa = 4.03 SEE690 pKa = 4.5 SEE692 pKa = 4.79 AEE694 pKa = 4.1 AQEE697 pKa = 4.1 EE698 pKa = 4.43 TQAGSVRR705 pKa = 11.84 EE706 pKa = 3.86 QLQQQLQEE714 pKa = 4.06 QFQLRR719 pKa = 11.84 RR720 pKa = 11.84 GLKK723 pKa = 10.11 CLFEE727 pKa = 4.13 QLVRR731 pKa = 11.84 TQQGVHH737 pKa = 6.15 VDD739 pKa = 3.41 PCLVV743 pKa = 3.12
Molecular weight: 87.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.531
IPC_protein 9.809
Toseland 10.189
ProMoST 9.897
Dawson 10.379
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.716
Grimsley 10.452
Solomon 10.409
Lehninger 10.365
Nozaki 10.204
DTASelect 10.058
Thurlkill 10.233
EMBOSS 10.584
Sillero 10.292
Patrickios 10.233
IPC_peptide 10.409
IPC2_peptide 8.931
IPC2.peptide.svr19 8.57
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1411
130
743
352.8
39.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.229 ± 2.238
1.488 ± 0.099
4.536 ± 0.606
5.174 ± 0.251
3.118 ± 1.138
8.434 ± 1.729
2.764 ± 0.689
3.827 ± 1.174
5.103 ± 1.14
7.158 ± 0.244
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.347 ± 0.328
4.607 ± 0.34
9.497 ± 1.439
4.04 ± 1.109
9.284 ± 0.752
7.3 ± 1.895
4.748 ± 1.164
4.607 ± 0.157
2.339 ± 0.412
3.402 ± 0.748
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here