Ruegeria denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ruegeria

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1IH33|A0A0P1IH33_9RHOB TPR_REGION domain-containing protein OS=Ruegeria denitrificans OX=1715692 GN=RUE5091_01455 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.22KK3 pKa = 10.39VLFASTALIATAGVAAADD21 pKa = 3.6SGVNFSGYY29 pKa = 9.98GRR31 pKa = 11.84FGIGYY36 pKa = 8.97QEE38 pKa = 4.32DD39 pKa = 3.84RR40 pKa = 11.84VGAVTNIVTPSNTATSATSVVVDD63 pKa = 3.68TDD65 pKa = 3.68DD66 pKa = 5.45AILVSRR72 pKa = 11.84FRR74 pKa = 11.84LNIDD78 pKa = 3.57GFTEE82 pKa = 3.82TDD84 pKa = 2.91GGVRR88 pKa = 11.84FEE90 pKa = 4.23GRR92 pKa = 11.84VRR94 pKa = 11.84LQADD98 pKa = 3.75EE99 pKa = 4.84DD100 pKa = 4.57SSTGEE105 pKa = 4.03ANSAGLNGARR115 pKa = 11.84FSVIYY120 pKa = 10.46GGLRR124 pKa = 11.84VDD126 pKa = 4.1AGNVAGSFDD135 pKa = 3.8NLANYY140 pKa = 9.13YY141 pKa = 9.98GNEE144 pKa = 3.81VGLEE148 pKa = 4.05LFAGQYY154 pKa = 10.74SGVNYY159 pKa = 10.59NFLAYY164 pKa = 10.47SSTGSGANAVFFQYY178 pKa = 11.24AVGDD182 pKa = 3.88FAFGASYY189 pKa = 7.99DD190 pKa = 3.62TRR192 pKa = 11.84TFAVNGSEE200 pKa = 4.27VSGDD204 pKa = 3.24RR205 pKa = 11.84WDD207 pKa = 3.83VSATYY212 pKa = 9.55TFNNITAAVAYY223 pKa = 8.31GQTDD227 pKa = 3.39SGVSGVSDD235 pKa = 3.86PSLTVLTLGGEE246 pKa = 3.93WGDD249 pKa = 3.57FGGTLFVADD258 pKa = 4.88DD259 pKa = 3.98ATEE262 pKa = 4.11VTATDD267 pKa = 3.56GTAWGLSASYY277 pKa = 11.03NLGAATTLLFTYY289 pKa = 10.92GDD291 pKa = 3.93GNADD295 pKa = 3.56ADD297 pKa = 4.19LQQVAIGAIYY307 pKa = 10.35DD308 pKa = 3.85LGGGASLRR316 pKa = 11.84GGIGVNDD323 pKa = 4.38CDD325 pKa = 3.61ICDD328 pKa = 3.89SQTVADD334 pKa = 6.04FGAQFNFF341 pKa = 3.8

Molecular weight: 35.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1IJ02|A0A0P1IJ02_9RHOB Uncharacterized protein OS=Ruegeria denitrificans OX=1715692 GN=RUE5091_03911 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4541

0

4541

1375832

29

4441

303.0

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.073 ± 0.042

0.96 ± 0.012

6.088 ± 0.029

6.039 ± 0.038

3.914 ± 0.022

8.395 ± 0.069

2.131 ± 0.021

5.443 ± 0.028

3.537 ± 0.032

9.88 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.017

3.01 ± 0.02

4.886 ± 0.028

3.558 ± 0.02

6.106 ± 0.037

5.542 ± 0.03

5.545 ± 0.025

7.331 ± 0.031

1.423 ± 0.016

2.35 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski