alpha proteobacterium U9-1i

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1PQJ3|A0A1V1PQJ3_9PROT Transcriptional regulator OS=alpha proteobacterium U9-1i OX=1605283 GN=U91I_02475 PE=3 SV=1
MM1 pKa = 8.37LEE3 pKa = 4.82DD4 pKa = 4.3RR5 pKa = 11.84PIAQKK10 pKa = 11.31ALVAAGAFVFVFGSAMAGTGFMLTGGFGYY39 pKa = 10.75ADD41 pKa = 3.81EE42 pKa = 5.06NNPSVRR48 pKa = 11.84SPDD51 pKa = 3.16YY52 pKa = 10.09GYY54 pKa = 10.92QYY56 pKa = 11.73AFAPQPHH63 pKa = 7.35EE64 pKa = 4.9DD65 pKa = 4.07PNSSEE70 pKa = 3.89ATYY73 pKa = 11.08SEE75 pKa = 4.76ASWTQPSSFDD85 pKa = 3.12NGAPLEE91 pKa = 4.25AWTPRR96 pKa = 11.84EE97 pKa = 4.04ARR99 pKa = 11.84SGQDD103 pKa = 3.12DD104 pKa = 4.12VQTVSLRR111 pKa = 11.84QEE113 pKa = 4.59EE114 pKa = 4.37ILDD117 pKa = 3.91AYY119 pKa = 10.54AADD122 pKa = 4.17DD123 pKa = 4.0FADD126 pKa = 4.31EE127 pKa = 4.29EE128 pKa = 4.74SGDD131 pKa = 3.73AFADD135 pKa = 3.44QSYY138 pKa = 8.32DD139 pKa = 3.18TDD141 pKa = 3.2EE142 pKa = 4.97SYY144 pKa = 11.81APVQDD149 pKa = 4.83EE150 pKa = 4.51YY151 pKa = 11.05PEE153 pKa = 4.18SLKK156 pKa = 10.24TEE158 pKa = 4.16AGAFF162 pKa = 3.61

Molecular weight: 17.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1PKY0|A0A1V1PKY0_9PROT Uncharacterized protein OS=alpha proteobacterium U9-1i OX=1605283 GN=U91I_00623 PE=4 SV=1
MM1 pKa = 7.39AKK3 pKa = 9.87KK4 pKa = 10.07VKK6 pKa = 10.07KK7 pKa = 9.75AAKK10 pKa = 10.02KK11 pKa = 9.79PMKK14 pKa = 9.17KK15 pKa = 7.84TARR18 pKa = 11.84KK19 pKa = 6.93TVRR22 pKa = 11.84KK23 pKa = 8.91AAKK26 pKa = 8.5KK27 pKa = 7.5TVRR30 pKa = 11.84KK31 pKa = 9.96AKK33 pKa = 9.91AKK35 pKa = 10.29KK36 pKa = 8.93PARR39 pKa = 11.84KK40 pKa = 8.78AAKK43 pKa = 9.64KK44 pKa = 7.51VTRR47 pKa = 11.84KK48 pKa = 8.89VAKK51 pKa = 10.07KK52 pKa = 8.82PAKK55 pKa = 9.95KK56 pKa = 9.99SVRR59 pKa = 11.84KK60 pKa = 8.51AAKK63 pKa = 9.76RR64 pKa = 11.84KK65 pKa = 5.63TARR68 pKa = 11.84KK69 pKa = 8.25AKK71 pKa = 9.72PVAAPVAPSVV81 pKa = 3.37

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4195

0

4195

1222329

37

3631

291.4

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.262 ± 0.067

0.78 ± 0.012

5.726 ± 0.032

5.8 ± 0.034

3.761 ± 0.025

8.473 ± 0.038

1.914 ± 0.019

4.749 ± 0.026

2.805 ± 0.037

9.676 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.018

2.689 ± 0.028

5.175 ± 0.026

3.173 ± 0.026

7.718 ± 0.042

5.218 ± 0.027

5.065 ± 0.036

7.122 ± 0.032

1.429 ± 0.017

2.138 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski