Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006]
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BND2|E7BND2_9VIRU C1 protein OS=Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006] OX=908010 GN=C1 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.12 ISNHH6 pKa = 6.52 FIHH9 pKa = 7.53 IFISSIYY16 pKa = 9.26 IRR18 pKa = 11.84 RR19 pKa = 11.84 TIKK22 pKa = 8.1 MTRR25 pKa = 11.84 FSKK28 pKa = 10.35 NRR30 pKa = 11.84 EE31 pKa = 4.0 GIVFKK36 pKa = 11.21 VDD38 pKa = 3.01 VRR40 pKa = 11.84 LMQQQRR46 pKa = 11.84 ISVQMQISSTRR57 pKa = 11.84 SPAISTKK64 pKa = 8.93 TFIIDD69 pKa = 3.13 YY70 pKa = 8.37 TYY72 pKa = 10.85 QQLHH76 pKa = 6.45 IPFDD80 pKa = 4.34 FNGLEE85 pKa = 4.12 GTITSTFKK93 pKa = 9.83 FHH95 pKa = 6.13 YY96 pKa = 8.58 WGSKK100 pKa = 9.77 AEE102 pKa = 4.51 EE103 pKa = 4.2 ILEE106 pKa = 3.83 EE107 pKa = 5.07 DD108 pKa = 4.3 IIHH111 pKa = 5.94 MVDD114 pKa = 3.9 IIIIEE119 pKa = 4.27 NPEE122 pKa = 3.78 IMGMDD127 pKa = 3.32 VNEE130 pKa = 4.77 PVTIDD135 pKa = 3.29 NKK137 pKa = 10.73 IIII140 pKa = 4.47
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.353
IPC2_protein 6.109
IPC_protein 6.313
Toseland 6.927
ProMoST 6.649
Dawson 6.62
Bjellqvist 6.504
Wikipedia 6.649
Rodwell 6.605
Grimsley 7.102
Solomon 6.634
Lehninger 6.634
Nozaki 6.825
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.088
Sillero 6.985
Patrickios 4.584
IPC_peptide 6.649
IPC2_peptide 6.839
IPC2.peptide.svr19 6.873
Protein with the highest isoelectric point:
>tr|E7BND2|E7BND2_9VIRU C1 protein OS=Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006] OX=908010 GN=C1 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.12 ISNHH6 pKa = 6.52 FIHH9 pKa = 7.53 IFISSIYY16 pKa = 9.26 IRR18 pKa = 11.84 RR19 pKa = 11.84 TIKK22 pKa = 8.1 MTRR25 pKa = 11.84 FSKK28 pKa = 10.35 NRR30 pKa = 11.84 EE31 pKa = 4.0 GIVFKK36 pKa = 11.21 VDD38 pKa = 3.01 VRR40 pKa = 11.84 LMQQQRR46 pKa = 11.84 ISVQMQISSTRR57 pKa = 11.84 SPAISTKK64 pKa = 8.93 TFIIDD69 pKa = 3.13 YY70 pKa = 8.37 TYY72 pKa = 10.85 QQLHH76 pKa = 6.45 IPFDD80 pKa = 4.34 FNGLEE85 pKa = 4.12 GTITSTFKK93 pKa = 9.83 FHH95 pKa = 6.13 YY96 pKa = 8.58 WGSKK100 pKa = 9.77 AEE102 pKa = 4.51 EE103 pKa = 4.2 ILEE106 pKa = 3.83 EE107 pKa = 5.07 DD108 pKa = 4.3 IIHH111 pKa = 5.94 MVDD114 pKa = 3.9 IIIIEE119 pKa = 4.27 NPEE122 pKa = 3.78 IMGMDD127 pKa = 3.32 VNEE130 pKa = 4.77 PVTIDD135 pKa = 3.29 NKK137 pKa = 10.73 IIII140 pKa = 4.47
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.353
IPC2_protein 6.109
IPC_protein 6.313
Toseland 6.927
ProMoST 6.649
Dawson 6.62
Bjellqvist 6.504
Wikipedia 6.649
Rodwell 6.605
Grimsley 7.102
Solomon 6.634
Lehninger 6.634
Nozaki 6.825
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.088
Sillero 6.985
Patrickios 4.584
IPC_peptide 6.649
IPC2_peptide 6.839
IPC2.peptide.svr19 6.873
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
140
140
140
140.0
16.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.429 ± 0.0
0.0 ± 0.0
5.0 ± 0.0
6.429 ± 0.0
6.429 ± 0.0
3.571 ± 0.0
3.571 ± 0.0
19.286 ± 0.0
5.714 ± 0.0
2.857 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.0 ± 0.0
4.286 ± 0.0
2.857 ± 0.0
5.0 ± 0.0
5.0 ± 0.0
7.857 ± 0.0
7.143 ± 0.0
5.0 ± 0.0
0.714 ± 0.0
2.857 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here