Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006] 
Average proteome isoelectric point is 6.35 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|E7BND2|E7BND2_9VIRU C1 protein OS=Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006] OX=908010 GN=C1 PE=4 SV=1MM1 pKa = 7.67  KK2 pKa = 10.12  ISNHH6 pKa = 6.52  FIHH9 pKa = 7.53  IFISSIYY16 pKa = 9.26  IRR18 pKa = 11.84  RR19 pKa = 11.84  TIKK22 pKa = 8.1  MTRR25 pKa = 11.84  FSKK28 pKa = 10.35  NRR30 pKa = 11.84  EE31 pKa = 4.0  GIVFKK36 pKa = 11.21  VDD38 pKa = 3.01  VRR40 pKa = 11.84  LMQQQRR46 pKa = 11.84  ISVQMQISSTRR57 pKa = 11.84  SPAISTKK64 pKa = 8.93  TFIIDD69 pKa = 3.13  YY70 pKa = 8.37  TYY72 pKa = 10.85  QQLHH76 pKa = 6.45  IPFDD80 pKa = 4.34  FNGLEE85 pKa = 4.12  GTITSTFKK93 pKa = 9.83  FHH95 pKa = 6.13  YY96 pKa = 8.58  WGSKK100 pKa = 9.77  AEE102 pKa = 4.51  EE103 pKa = 4.2  ILEE106 pKa = 3.83  EE107 pKa = 5.07  DD108 pKa = 4.3  IIHH111 pKa = 5.94  MVDD114 pKa = 3.9  IIIIEE119 pKa = 4.27  NPEE122 pKa = 3.78  IMGMDD127 pKa = 3.32  VNEE130 pKa = 4.77  PVTIDD135 pKa = 3.29  NKK137 pKa = 10.73  IIII140 pKa = 4.47  
 16.44 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  6.353 
IPC2_protein 6.109 
IPC_protein 6.313 
Toseland    6.927 
ProMoST     6.649 
Dawson      6.62 
Bjellqvist  6.504 
Wikipedia   6.649 
Rodwell     6.605 
Grimsley    7.102 
Solomon     6.634 
Lehninger   6.634 
Nozaki      6.825 
DTASelect   7.059 
Thurlkill   7.088 
EMBOSS      7.088 
Sillero     6.985 
Patrickios  4.584 
IPC_peptide 6.649 
IPC2_peptide  6.839 
IPC2.peptide.svr19  6.873 
 Protein with the highest isoelectric point: 
>tr|E7BND2|E7BND2_9VIRU C1 protein OS=Bhendi yellow vein India betasatellite [India:Aurangabad:OY164:2006] OX=908010 GN=C1 PE=4 SV=1MM1 pKa = 7.67  KK2 pKa = 10.12  ISNHH6 pKa = 6.52  FIHH9 pKa = 7.53  IFISSIYY16 pKa = 9.26  IRR18 pKa = 11.84  RR19 pKa = 11.84  TIKK22 pKa = 8.1  MTRR25 pKa = 11.84  FSKK28 pKa = 10.35  NRR30 pKa = 11.84  EE31 pKa = 4.0  GIVFKK36 pKa = 11.21  VDD38 pKa = 3.01  VRR40 pKa = 11.84  LMQQQRR46 pKa = 11.84  ISVQMQISSTRR57 pKa = 11.84  SPAISTKK64 pKa = 8.93  TFIIDD69 pKa = 3.13  YY70 pKa = 8.37  TYY72 pKa = 10.85  QQLHH76 pKa = 6.45  IPFDD80 pKa = 4.34  FNGLEE85 pKa = 4.12  GTITSTFKK93 pKa = 9.83  FHH95 pKa = 6.13  YY96 pKa = 8.58  WGSKK100 pKa = 9.77  AEE102 pKa = 4.51  EE103 pKa = 4.2  ILEE106 pKa = 3.83  EE107 pKa = 5.07  DD108 pKa = 4.3  IIHH111 pKa = 5.94  MVDD114 pKa = 3.9  IIIIEE119 pKa = 4.27  NPEE122 pKa = 3.78  IMGMDD127 pKa = 3.32  VNEE130 pKa = 4.77  PVTIDD135 pKa = 3.29  NKK137 pKa = 10.73  IIII140 pKa = 4.47  
 16.44 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  6.353 
IPC2_protein 6.109 
IPC_protein 6.313 
Toseland    6.927 
ProMoST     6.649 
Dawson      6.62 
Bjellqvist  6.504 
Wikipedia   6.649 
Rodwell     6.605 
Grimsley    7.102 
Solomon     6.634 
Lehninger   6.634 
Nozaki      6.825 
DTASelect   7.059 
Thurlkill   7.088 
EMBOSS      7.088 
Sillero     6.985 
Patrickios  4.584 
IPC_peptide 6.649 
IPC2_peptide  6.839 
IPC2.peptide.svr19  6.873 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        1 
0
1 
140
140
140
140.0
16.44
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        1.429 ± 0.0
0.0 ± 0.0
5.0 ± 0.0
6.429 ± 0.0
6.429 ± 0.0
3.571 ± 0.0
3.571 ± 0.0
19.286 ± 0.0
5.714 ± 0.0
2.857 ± 0.0
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        5.0 ± 0.0
4.286 ± 0.0
2.857 ± 0.0
5.0 ± 0.0
5.0 ± 0.0
7.857 ± 0.0
7.143 ± 0.0
5.0 ± 0.0
0.714 ± 0.0
2.857 ± 0.0
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here