Bacillus sp. V5-8f

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5MCK4|A0A2N5MCK4_9BACI Uncharacterized protein OS=Bacillus sp. V5-8f OX=2053044 GN=CUU64_20955 PE=4 SV=1
MM1 pKa = 7.26MPNANILTEE10 pKa = 4.07SDD12 pKa = 3.72SYY14 pKa = 10.97GTEE17 pKa = 3.88SYY19 pKa = 10.87QYY21 pKa = 10.35NQNNDD26 pKa = 2.96VTSITDD32 pKa = 3.42TEE34 pKa = 4.75DD35 pKa = 3.22GVTSIAYY42 pKa = 9.67DD43 pKa = 3.44GLNAVSEE50 pKa = 4.32TDD52 pKa = 3.26HH53 pKa = 6.28NGKK56 pKa = 9.78VSSVAKK62 pKa = 10.34YY63 pKa = 10.58DD64 pKa = 3.47EE65 pKa = 4.77SGNGNLIEE73 pKa = 4.74SSDD76 pKa = 4.0NLGSASNLLINSSFEE91 pKa = 4.44DD92 pKa = 5.0GVASWKK98 pKa = 10.34HH99 pKa = 4.33

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5MG46|A0A2N5MG46_9BACI Uncharacterized protein OS=Bacillus sp. V5-8f OX=2053044 GN=CUU64_13430 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4122

0

4122

1167400

23

2835

283.2

31.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.187 ± 0.046

0.717 ± 0.011

4.994 ± 0.029

7.451 ± 0.039

4.492 ± 0.032

7.189 ± 0.04

2.082 ± 0.018

7.844 ± 0.039

6.921 ± 0.036

9.577 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.016

4.465 ± 0.028

3.741 ± 0.027

3.647 ± 0.029

4.186 ± 0.03

6.146 ± 0.029

5.297 ± 0.023

6.929 ± 0.037

0.979 ± 0.015

3.404 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski