Sinocyclocheilus anshuiensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii;

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A671M871|A0A671M871_9TELE FXYD domain-containing ion transport regulator OS=Sinocyclocheilus anshuiensis OX=1608454 GN=LOC107665652 PE=3 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84PVEE5 pKa = 4.18GQCCGSCPSLPPIIIPGMDD24 pKa = 3.31SVEE27 pKa = 4.13LCTTQEE33 pKa = 4.28DD34 pKa = 4.73GCLPTDD40 pKa = 3.69GEE42 pKa = 4.15MGYY45 pKa = 9.99PEE47 pKa = 4.59AQAMDD52 pKa = 4.59EE53 pKa = 4.21YY54 pKa = 10.51PIQRR58 pKa = 11.84KK59 pKa = 8.62GDD61 pKa = 3.62RR62 pKa = 11.84LPFQIFPDD70 pKa = 3.82PVEE73 pKa = 4.32VVLSTEE79 pKa = 4.12GCLTCAPQEE88 pKa = 4.25KK89 pKa = 9.75EE90 pKa = 4.01RR91 pKa = 11.84LPSIVVEE98 pKa = 4.1PTDD101 pKa = 3.44VSEE104 pKa = 4.56VEE106 pKa = 4.27SGEE109 pKa = 4.3LRR111 pKa = 11.84WPPEE115 pKa = 4.09DD116 pKa = 3.39MDD118 pKa = 4.13FDD120 pKa = 4.24EE121 pKa = 7.53DD122 pKa = 3.63EE123 pKa = 5.21DD124 pKa = 5.44LFLEE128 pKa = 4.55QCIPPANIADD138 pKa = 3.45WGEE141 pKa = 4.12GEE143 pKa = 4.44EE144 pKa = 4.17EE145 pKa = 3.97SSIIEE150 pKa = 3.78NHH152 pKa = 5.08QQNTSLLDD160 pKa = 3.61LPRR163 pKa = 11.84SSGSSFEE170 pKa = 4.23YY171 pKa = 10.84

Molecular weight: 18.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A671QUH7|A0A671QUH7_9TELE Isoform of A0A671QUH6 Uncharacterized protein OS=Sinocyclocheilus anshuiensis OX=1608454 PE=4 SV=1
MM1 pKa = 7.45NMKK4 pKa = 10.17QNPTLHH10 pKa = 6.56IPPSKK15 pKa = 10.67GGSRR19 pKa = 11.84CPNKK23 pKa = 9.4PKK25 pKa = 10.12KK26 pKa = 9.78SRR28 pKa = 11.84RR29 pKa = 11.84VVEE32 pKa = 3.81GTGRR36 pKa = 11.84VGSHH40 pKa = 6.19GGAGRR45 pKa = 11.84SGSHH49 pKa = 6.22GGAGRR54 pKa = 11.84AGQPGGAGRR63 pKa = 11.84AGSHH67 pKa = 6.17GGAGRR72 pKa = 11.84AGSHH76 pKa = 6.17GGAGRR81 pKa = 11.84AGSHH85 pKa = 6.17GGAGRR90 pKa = 11.84AGSHH94 pKa = 6.17GGAGRR99 pKa = 11.84AGSHH103 pKa = 6.17GGAGRR108 pKa = 11.84AGSHH112 pKa = 6.17GGAGRR117 pKa = 11.84AGSHH121 pKa = 6.17GGAGRR126 pKa = 11.84AGSHH130 pKa = 6.17GGAGRR135 pKa = 11.84AGSHH139 pKa = 6.17GGAGRR144 pKa = 11.84AGSHH148 pKa = 6.17GGAGRR153 pKa = 11.84AGSHH157 pKa = 6.17GGAGRR162 pKa = 11.84AGSHH166 pKa = 6.17GGAGRR171 pKa = 11.84AGSHH175 pKa = 6.17GGAGRR180 pKa = 11.84AGSHH184 pKa = 6.17GGAGRR189 pKa = 11.84AGSHH193 pKa = 6.17GGAGRR198 pKa = 11.84AGSHH202 pKa = 6.17GGAGRR207 pKa = 11.84AGSHH211 pKa = 6.17GGAGRR216 pKa = 11.84AGSHH220 pKa = 6.17GGAGRR225 pKa = 11.84AGSHH229 pKa = 6.17GGAGRR234 pKa = 11.84AGSHH238 pKa = 6.17GGAGRR243 pKa = 11.84AGSHH247 pKa = 6.17GGAGRR252 pKa = 11.84AGSHH256 pKa = 6.17GGAGRR261 pKa = 11.84AGSHH265 pKa = 6.17GGAGRR270 pKa = 11.84AGSHH274 pKa = 6.17GGAGRR279 pKa = 11.84AGSHH283 pKa = 6.17GGAGRR288 pKa = 11.84AGSHH292 pKa = 6.17GGAGRR297 pKa = 11.84AGSHH301 pKa = 6.17GGAGRR306 pKa = 11.84AGSHH310 pKa = 6.17GGAGRR315 pKa = 11.84AGSHH319 pKa = 6.17GGAGRR324 pKa = 11.84AGSHH328 pKa = 6.17GGAGRR333 pKa = 11.84AGSHH337 pKa = 6.17GGAGRR342 pKa = 11.84AGSHH346 pKa = 6.17GGAGRR351 pKa = 11.84AGSHH355 pKa = 6.17GGAGRR360 pKa = 11.84AGSHH364 pKa = 6.17GGAGRR369 pKa = 11.84AGSHH373 pKa = 6.17GGAGRR378 pKa = 11.84AGRR381 pKa = 11.84RR382 pKa = 11.84GTGRR386 pKa = 11.84VGSHH390 pKa = 6.19GGAGRR395 pKa = 11.84SGKK398 pKa = 9.14IHH400 pKa = 6.37EE401 pKa = 5.24GKK403 pKa = 11.19GNMKK407 pKa = 8.96TT408 pKa = 3.4

Molecular weight: 35.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43571

56704

100275

62118981

18

8680

619.5

69.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.313 ± 0.006

2.306 ± 0.006

5.261 ± 0.005

6.893 ± 0.008

3.865 ± 0.005

6.027 ± 0.009

2.671 ± 0.003

4.837 ± 0.005

5.898 ± 0.008

9.708 ± 0.009

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.003

4.026 ± 0.004

5.268 ± 0.009

4.684 ± 0.006

5.438 ± 0.005

8.274 ± 0.009

5.526 ± 0.005

6.408 ± 0.006

1.17 ± 0.002

2.923 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski