Proteome-pI 2.0 - Proteome Isoelectric Point Database

Coccidioides immitis (strain RS) (Valley fever fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Onygenales incertae sedis; Coccidioides; Coccidioides immitis

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 9712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|J3K7Z4|J3K7Z4_COCIM WAPL domain-containing protein OS=Coccidioides immitis (strain RS) OX=246410 GN=CIMG_06350 PE=4 SV=2

MM1 pKa = 7.76ASIQTRR7 pKa = 11.84TDD9 pKa = 3.14VAPTGTSAVTSAVPTACGATTYY31 pKa = 10.2DD32 pKa = 4.32IPTKK36 pKa = 10.53DD37 pKa = 3.26AACAVPGTEE46 pKa = 5.17HH47 pKa = 7.14KK48 pKa = 10.98DD49 pKa = 3.73SMEE52 pKa = 4.27KK53 pKa = 10.76CCDD56 pKa = 3.65APVVTYY62 pKa = 11.16NEE64 pKa = 4.46GCGMYY69 pKa = 10.13CLASGGTVGDD79 pKa = 4.12LVKK82 pKa = 10.84CLIGDD87 pKa = 4.79GIADD91 pKa = 3.57GKK93 pKa = 9.37VFCNKK98 pKa = 8.87EE99 pKa = 3.83MNATATTTPTPTGRR113 pKa = 11.84DD114 pKa = 3.69DD115 pKa = 6.63DD116 pKa = 6.42DD117 pKa = 7.49DD118 pKa = 7.45DD119 pKa = 7.62DD120 pKa = 7.59DD121 pKa = 7.12DD122 pKa = 6.34DD123 pKa = 5.5EE124 pKa = 4.63PTNTDD129 pKa = 3.0GSPASTSSSAALANLPPQTLSKK151 pKa = 10.03PAIGVLFTLFFSMFAGVVFAA171 pKa = 6.02

Molecular weight: 17.42 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|J3K8M6|J3K8M6_COCIM Uncharacterized protein OS=Coccidioides immitis (strain RS) OX=246410 GN=CIMG_06666 PE=4 SV=2

MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.24 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
9587	125	9712	4213409	39	8032	433.8	48.22

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
8.031 ± 0.022	1.316 ± 0.011	5.521 ± 0.016	6.298 ± 0.025	3.716 ± 0.015	6.587 ± 0.021	2.453 ± 0.012	5.088 ± 0.017	5.13 ± 0.02	8.97 ± 0.028

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.136 ± 0.009	3.776 ± 0.012	6.25 ± 0.029	4.041 ± 0.016	6.545 ± 0.02	8.597 ± 0.031	5.665 ± 0.014	5.855 ± 0.018	1.362 ± 0.011	2.664 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski