SAR116 cluster alpha proteobacterium HIMB100

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Alphaproteobacteria incertae sedis; SAR116 cluster; unclassified SAR116 cluster

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5ZYX4|G5ZYX4_9PROT Putative permease OS=SAR116 cluster alpha proteobacterium HIMB100 OX=909943 GN=HIMB100_00011470 PE=3 SV=1
MM1 pKa = 7.36IRR3 pKa = 11.84FALTLLFLVASGLALAGTATSPSVTEE29 pKa = 3.99GNNAVVTFDD38 pKa = 4.42LGYY41 pKa = 7.53TAPSGGVEE49 pKa = 4.38VYY51 pKa = 10.42VSANSATATLGTDD64 pKa = 3.98FTVAASQLGYY74 pKa = 9.04RR75 pKa = 11.84TIAAGNQTLTVTAAIISDD93 pKa = 3.68GVTEE97 pKa = 4.22GNEE100 pKa = 3.92VFPVAFLEE108 pKa = 4.32NVSSNATFDD117 pKa = 3.42GGATEE122 pKa = 4.62WSDD125 pKa = 3.02KK126 pKa = 10.26TIRR129 pKa = 11.84TQPNVDD135 pKa = 4.25FTSSSPPIGNNVLHH149 pKa = 6.4IQNKK153 pKa = 7.17EE154 pKa = 3.57EE155 pKa = 4.48PYY157 pKa = 10.59RR158 pKa = 11.84SVSLNSGTDD167 pKa = 3.3YY168 pKa = 11.03DD169 pKa = 5.56IEE171 pKa = 4.6FDD173 pKa = 3.41WSEE176 pKa = 4.21ANADD180 pKa = 4.08GYY182 pKa = 11.67GSYY185 pKa = 10.99CDD187 pKa = 4.32DD188 pKa = 3.65SSVYY192 pKa = 10.83SGFVNAADD200 pKa = 3.85RR201 pKa = 11.84LVLQVIDD208 pKa = 4.37TVDD211 pKa = 3.0SSNNVSFTIDD221 pKa = 3.01NNMGNGAAQTSGDD234 pKa = 4.33SIFNFQGKK242 pKa = 9.51ISNLSWTGNSAAQVKK257 pKa = 8.7FVVYY261 pKa = 8.65DD262 pKa = 3.48TTNRR266 pKa = 11.84QYY268 pKa = 11.2EE269 pKa = 5.2GYY271 pKa = 9.56CGYY274 pKa = 10.99VIDD277 pKa = 4.63NFRR280 pKa = 11.84INKK283 pKa = 9.76SPVQTTVTISDD294 pKa = 4.59PDD296 pKa = 3.82TTPPTVSISSTTVTSGNTSNDD317 pKa = 2.8SSIALSFTLSEE328 pKa = 4.11TATDD332 pKa = 5.69FIASDD337 pKa = 3.37ISVTT341 pKa = 3.54

Molecular weight: 35.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5ZWN6|G5ZWN6_9PROT Major Facilitator Superfamily transporter OS=SAR116 cluster alpha proteobacterium HIMB100 OX=909943 GN=HIMB100_00004300 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 10.73ALILAILIMFVLLILLRR20 pKa = 11.84RR21 pKa = 11.84IPAIRR26 pKa = 11.84TWMNNLLRR34 pKa = 11.84QPLVRR39 pKa = 11.84SILFQGLWRR48 pKa = 11.84LIRR51 pKa = 11.84FLIFRR56 pKa = 11.84RR57 pKa = 3.83

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2334

0

2334

749038

29

7426

320.9

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.994 ± 0.062

1.136 ± 0.019

6.145 ± 0.051

5.364 ± 0.045

3.983 ± 0.042

7.975 ± 0.061

2.258 ± 0.03

5.799 ± 0.038

3.88 ± 0.041

10.183 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.029

3.149 ± 0.062

4.453 ± 0.048

4.097 ± 0.032

5.534 ± 0.051

6.344 ± 0.06

5.564 ± 0.063

6.809 ± 0.048

1.232 ± 0.023

2.426 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski