Lactobacillus phage LF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lafunavirus; Lactobacillus virus LF1

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9LUI9|E9LUI9_9CAUD Major tail protein OS=Lactobacillus phage LF1 OX=947980 PE=4 SV=1
MM1 pKa = 7.62SLPEE5 pKa = 4.38IEE7 pKa = 4.83IADD10 pKa = 4.23LLSANSGLVSLMASLRR26 pKa = 11.84QSKK29 pKa = 10.4LDD31 pKa = 3.84YY32 pKa = 10.82IPIFTSTPDD41 pKa = 3.04DD42 pKa = 3.72TFIKK46 pKa = 10.18RR47 pKa = 11.84SSAPWIRR54 pKa = 11.84VTPIPGDD61 pKa = 3.94DD62 pKa = 3.87EE63 pKa = 5.18VSADD67 pKa = 4.25DD68 pKa = 4.35GRR70 pKa = 11.84LLEE73 pKa = 4.3FPRR76 pKa = 11.84VEE78 pKa = 3.12VDD80 pKa = 3.01YY81 pKa = 10.41WVRR84 pKa = 11.84DD85 pKa = 3.42EE86 pKa = 4.81DD87 pKa = 3.97VEE89 pKa = 5.06SIEE92 pKa = 4.47EE93 pKa = 4.16MQEE96 pKa = 3.76MIYY99 pKa = 10.98DD100 pKa = 3.91SLVTNGWSRR109 pKa = 11.84YY110 pKa = 6.5YY111 pKa = 10.56VYY113 pKa = 10.36RR114 pKa = 11.84YY115 pKa = 10.19SDD117 pKa = 4.19PDD119 pKa = 3.44LSGCTMIVNKK129 pKa = 10.35FEE131 pKa = 4.23GYY133 pKa = 9.96KK134 pKa = 9.93MKK136 pKa = 10.91GG137 pKa = 3.07

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9LUN1|E9LUN1_9CAUD HAD-superfamily hydrolase OS=Lactobacillus phage LF1 OX=947980 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84FEE4 pKa = 4.68FDD6 pKa = 3.38IEE8 pKa = 4.11PVEE11 pKa = 4.11QARR14 pKa = 11.84PRR16 pKa = 11.84ATRR19 pKa = 11.84MSKK22 pKa = 10.53GIRR25 pKa = 11.84LYY27 pKa = 10.73DD28 pKa = 3.69PKK30 pKa = 10.87KK31 pKa = 8.14VTIFKK36 pKa = 10.34RR37 pKa = 11.84QLGMLAKK44 pKa = 10.14QQMLDD49 pKa = 3.59RR50 pKa = 11.84GLEE53 pKa = 4.1LYY55 pKa = 10.46DD56 pKa = 4.83GPLEE60 pKa = 4.21VCMEE64 pKa = 4.77FYY66 pKa = 10.88RR67 pKa = 11.84PVQTSISKK75 pKa = 10.23KK76 pKa = 7.48EE77 pKa = 3.68QARR80 pKa = 11.84RR81 pKa = 11.84LSGVHH86 pKa = 6.29RR87 pKa = 11.84PTVKK91 pKa = 10.19PDD93 pKa = 3.17LDD95 pKa = 4.53KK96 pKa = 11.12IILNRR101 pKa = 11.84LRR103 pKa = 11.84TPP105 pKa = 3.55

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12752

32

1430

223.7

25.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.132 ± 0.748

0.612 ± 0.123

6.822 ± 0.452

5.756 ± 0.423

3.403 ± 0.235

6.266 ± 0.386

1.49 ± 0.177

5.928 ± 0.253

7.63 ± 0.351

8.148 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.209

6.07 ± 0.24

2.87 ± 0.253

4.87 ± 0.34

4.211 ± 0.335

7.332 ± 0.449

7.105 ± 0.414

5.827 ± 0.196

1.372 ± 0.101

3.662 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski