Torque teno virus (isolate Japanese macaque/Japan/Mf-TTV9/2000) (TTV)
Average proteome isoelectric point is 7.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9DUC5|YORF2_TTVV9 Uncharacterized ORF2 protein OS=Torque teno virus (isolate Japanese macaque/Japan/Mf-TTV9/2000) OX=687364 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.08 TNQWEE6 pKa = 4.49 PTTLNAWGRR15 pKa = 11.84 EE16 pKa = 4.2 EE17 pKa = 3.68 AWLRR21 pKa = 11.84 SVVDD25 pKa = 3.27 SHH27 pKa = 7.77 QSFCGCNDD35 pKa = 3.53 PGFHH39 pKa = 7.06 LGLLLHH45 pKa = 6.36 SAGRR49 pKa = 11.84 HH50 pKa = 4.73 LGGPGGPQPPPAPGGPGVGGEE71 pKa = 4.33 GPRR74 pKa = 11.84 RR75 pKa = 11.84 FLPLPGLPANPEE87 pKa = 4.19 EE88 pKa = 5.15 PGHH91 pKa = 5.79 QRR93 pKa = 11.84 PPCPGGPGDD102 pKa = 3.93 AGGAGGVDD110 pKa = 3.3 AEE112 pKa = 4.52 TGEE115 pKa = 4.67 GEE117 pKa = 4.21 WRR119 pKa = 11.84 PEE121 pKa = 4.45 DD122 pKa = 3.64 IAEE125 pKa = 4.48 LLDD128 pKa = 3.79 GLEE131 pKa = 4.48 DD132 pKa = 3.6 AEE134 pKa = 4.78 KK135 pKa = 10.71 RR136 pKa = 3.79
Molecular weight: 14.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.697
IPC2_protein 4.698
IPC_protein 4.584
Toseland 4.469
ProMoST 4.685
Dawson 4.533
Bjellqvist 4.673
Wikipedia 4.38
Rodwell 4.444
Grimsley 4.38
Solomon 4.533
Lehninger 4.482
Nozaki 4.647
DTASelect 4.762
Thurlkill 4.469
EMBOSS 4.406
Sillero 4.711
Patrickios 3.897
IPC_peptide 4.533
IPC2_peptide 4.711
IPC2.peptide.svr19 4.636
Protein with the highest isoelectric point:
>sp|Q9DUC5|YORF2_TTVV9 Uncharacterized ORF2 protein OS=Torque teno virus (isolate Japanese macaque/Japan/Mf-TTV9/2000) OX=687364 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.56 PWWPWRR7 pKa = 11.84 RR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 WRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 GNWRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 VAPRR26 pKa = 11.84 RR27 pKa = 11.84 YY28 pKa = 9.42 RR29 pKa = 11.84 RR30 pKa = 11.84 TARR33 pKa = 11.84 RR34 pKa = 11.84 ARR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 8.55 AVRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 VRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 GYY55 pKa = 7.43 YY56 pKa = 8.41 RR57 pKa = 11.84 RR58 pKa = 11.84 YY59 pKa = 9.0 RR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 YY65 pKa = 9.64 KK66 pKa = 10.18 RR67 pKa = 11.84 RR68 pKa = 11.84 LLILKK73 pKa = 8.65 QWQPTTNLRR82 pKa = 11.84 LTVKK86 pKa = 10.46 GLIPVVVMGKK96 pKa = 10.63 GKK98 pKa = 7.51 TQNNFGQWEE107 pKa = 4.14 QTVPLEE113 pKa = 4.26 GEE115 pKa = 4.33 SYY117 pKa = 10.84 GGSFTIRR124 pKa = 11.84 KK125 pKa = 7.25 FTLQTLYY132 pKa = 11.08 EE133 pKa = 5.06 DD134 pKa = 3.85 YY135 pKa = 11.35 LKK137 pKa = 10.97 LRR139 pKa = 11.84 NRR141 pKa = 11.84 WSRR144 pKa = 11.84 SNTDD148 pKa = 3.38 LEE150 pKa = 4.73 LIRR153 pKa = 11.84 YY154 pKa = 6.71 TGLNLRR160 pKa = 11.84 LYY162 pKa = 9.52 RR163 pKa = 11.84 HH164 pKa = 6.19 EE165 pKa = 4.22 FSDD168 pKa = 4.59 YY169 pKa = 9.26 IVHH172 pKa = 6.45 YY173 pKa = 10.48 SLEE176 pKa = 4.29 TPMEE180 pKa = 4.39 VGLEE184 pKa = 4.1 SHH186 pKa = 6.51 MLAHH190 pKa = 6.84 PLKK193 pKa = 9.87 MIMSSKK199 pKa = 10.28 HH200 pKa = 3.47 VTVPSLLTKK209 pKa = 10.41 KK210 pKa = 10.45 GGRR213 pKa = 11.84 RR214 pKa = 11.84 YY215 pKa = 10.07 LRR217 pKa = 11.84 LKK219 pKa = 10.38 IPPPKK224 pKa = 10.73 LMMTQWYY231 pKa = 7.62 FQKK234 pKa = 10.44 EE235 pKa = 4.18 FCQVGLVLLSISTATLMHH253 pKa = 6.97 PWMAPFVNSPALTIYY268 pKa = 10.53 AVNHH272 pKa = 5.13 KK273 pKa = 8.76 TYY275 pKa = 10.95 SDD277 pKa = 3.6 MSILPSNNQGSTKK290 pKa = 10.67 NEE292 pKa = 4.45 LIEE295 pKa = 4.04 TLYY298 pKa = 10.24 TAEE301 pKa = 3.93 HH302 pKa = 6.85 TYY304 pKa = 11.39 NPMAQRR310 pKa = 11.84 IWGNIKK316 pKa = 9.45 PQNTNFTPEE325 pKa = 4.11 NYY327 pKa = 7.74 WNKK330 pKa = 9.18 WNEE333 pKa = 3.33 IHH335 pKa = 7.15 TKK337 pKa = 10.09 IKK339 pKa = 9.91 TNRR342 pKa = 11.84 QSEE345 pKa = 4.4 LTQLKK350 pKa = 9.35 QMRR353 pKa = 11.84 KK354 pKa = 8.82 RR355 pKa = 11.84 LEE357 pKa = 3.93 LTTEE361 pKa = 3.98 NDD363 pKa = 3.48 YY364 pKa = 11.36 QDD366 pKa = 4.04 PNFGLSYY373 pKa = 11.09 GLYY376 pKa = 10.2 SPLLLYY382 pKa = 10.43 PEE384 pKa = 4.91 MYY386 pKa = 10.32 FPEE389 pKa = 4.1 QSKK392 pKa = 10.38 VYY394 pKa = 10.48 QKK396 pKa = 11.26 ARR398 pKa = 11.84 YY399 pKa = 8.99 NPLLDD404 pKa = 3.43 QGIGNVVWTEE414 pKa = 3.67 PLTKK418 pKa = 9.38 KK419 pKa = 8.16 TCDD422 pKa = 3.72 YY423 pKa = 11.34 ASQAYY428 pKa = 9.38 NVIKK432 pKa = 10.44 DD433 pKa = 3.63 APLYY437 pKa = 10.25 LALFGYY443 pKa = 10.22 IDD445 pKa = 3.86 ICSKK449 pKa = 10.19 LAKK452 pKa = 10.18 DD453 pKa = 3.32 KK454 pKa = 11.38 SFYY457 pKa = 10.58 LSNRR461 pKa = 11.84 VCVKK465 pKa = 10.4 CPYY468 pKa = 9.18 TVPQLLSKK476 pKa = 9.82 TNATLGHH483 pKa = 6.38 VILSEE488 pKa = 3.75 NFMRR492 pKa = 11.84 GLVPSKK498 pKa = 10.71 DD499 pKa = 3.79 SYY501 pKa = 10.85 VPLYY505 pKa = 10.22 MRR507 pKa = 11.84 GKK509 pKa = 8.43 WYY511 pKa = 9.56 PSIYY515 pKa = 9.48 HH516 pKa = 4.83 QEE518 pKa = 3.98 EE519 pKa = 4.21 VIEE522 pKa = 5.21 AIVSSGPFVPRR533 pKa = 11.84 DD534 pKa = 3.7 QITKK538 pKa = 10.48 SLDD541 pKa = 2.95 ITIGCRR547 pKa = 11.84 FGFRR551 pKa = 11.84 IGGNLLNPKK560 pKa = 9.77 QVGDD564 pKa = 3.63 PCKK567 pKa = 10.47 QPTHH571 pKa = 6.96 PLPAPGGGDD580 pKa = 3.71 LLRR583 pKa = 11.84 AVQVSDD589 pKa = 3.41 PRR591 pKa = 11.84 KK592 pKa = 10.09 VGIQFHH598 pKa = 6.34 PWDD601 pKa = 4.34 LRR603 pKa = 11.84 RR604 pKa = 11.84 GMLSTSSIKK613 pKa = 10.58 RR614 pKa = 11.84 MLQDD618 pKa = 3.36 SDD620 pKa = 4.27 DD621 pKa = 4.52 DD622 pKa = 4.29 EE623 pKa = 6.82 SIEE626 pKa = 4.38 FPPKK630 pKa = 10.08 AWPGDD635 pKa = 3.66 PVPVGRR641 pKa = 11.84 TLEE644 pKa = 4.19 EE645 pKa = 4.08 RR646 pKa = 11.84 CSSSLYY652 pKa = 10.64 HH653 pKa = 7.08 LLQEE657 pKa = 4.58 QATPPPFKK665 pKa = 10.59 KK666 pKa = 10.05 PRR668 pKa = 11.84 TEE670 pKa = 3.94 DD671 pKa = 3.19 QEE673 pKa = 4.38 EE674 pKa = 4.27 NPEE677 pKa = 4.07 EE678 pKa = 4.2 TTQLQLFQEE687 pKa = 4.63 LQRR690 pKa = 11.84 QRR692 pKa = 11.84 EE693 pKa = 4.11 LQLQLKK699 pKa = 9.86 RR700 pKa = 11.84 GFRR703 pKa = 11.84 GLVEE707 pKa = 4.32 EE708 pKa = 4.65 MIKK711 pKa = 9.06 SHH713 pKa = 6.32 RR714 pKa = 11.84 HH715 pKa = 4.74 LALDD719 pKa = 4.69 PYY721 pKa = 11.19 LKK723 pKa = 10.77
Molecular weight: 85.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 9.633
IPC_protein 10.043
Toseland 10.204
ProMoST 9.955
Dawson 10.409
Bjellqvist 10.116
Wikipedia 10.613
Rodwell 10.716
Grimsley 10.496
Solomon 10.438
Lehninger 10.394
Nozaki 10.204
DTASelect 10.116
Thurlkill 10.262
EMBOSS 10.613
Sillero 10.321
Patrickios 10.189
IPC_peptide 10.438
IPC2_peptide 8.946
IPC2.peptide.svr19 8.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
945
86
723
315.0
36.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.81 ± 1.197
1.27 ± 0.326
3.598 ± 0.547
5.926 ± 1.23
2.54 ± 0.719
7.407 ± 4.262
2.328 ± 0.481
3.492 ± 1.318
6.032 ± 1.99
10.37 ± 1.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.58
4.021 ± 0.451
7.937 ± 2.321
4.868 ± 0.708
9.947 ± 1.402
7.407 ± 4.841
5.608 ± 1.061
4.444 ± 0.892
2.434 ± 0.326
4.127 ± 1.878
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here