European turkey coronavirus 080385d
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2ZXR0|A0A0S2ZXR0_9GAMC Growth factor-like peptide (Fragment) OS=European turkey coronavirus 080385d OX=1763410 PE=3 SV=1
MM1 pKa = 7.88 LDD3 pKa = 3.68 FEE5 pKa = 5.94 AIIEE9 pKa = 4.19 TGYY12 pKa = 11.47 GVIQQISFDD21 pKa = 3.92 LQHH24 pKa = 6.75 ISSVLNTEE32 pKa = 4.09 LFDD35 pKa = 4.14 PFEE38 pKa = 4.4 FCVYY42 pKa = 10.39 RR43 pKa = 11.84 GGNYY47 pKa = 8.75 WEE49 pKa = 5.19 LEE51 pKa = 4.23 SADD54 pKa = 4.2 EE55 pKa = 4.75 FSGDD59 pKa = 4.17 DD60 pKa = 3.71 EE61 pKa = 4.51 FVEE64 pKa = 4.53
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.006
IPC2_protein 3.516
IPC_protein 3.376
Toseland 3.223
ProMoST 3.541
Dawson 3.389
Bjellqvist 3.643
Wikipedia 3.338
Rodwell 3.249
Grimsley 3.147
Solomon 3.325
Lehninger 3.274
Nozaki 3.541
DTASelect 3.643
Thurlkill 3.3
EMBOSS 3.338
Sillero 3.516
Patrickios 0.172
IPC_peptide 3.325
IPC2_peptide 3.478
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|A0A0S2ZXV4|A0A0S2ZXV4_9GAMC 4b OS=European turkey coronavirus 080385d OX=1763410 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.77 WLTSFGRR9 pKa = 11.84 AVISCYY15 pKa = 10.12 KK16 pKa = 10.68 ALLLTQLRR24 pKa = 11.84 VLDD27 pKa = 4.68 RR28 pKa = 11.84 LILDD32 pKa = 4.43 HH33 pKa = 6.7 GPKK36 pKa = 8.96 RR37 pKa = 11.84 TLTCARR43 pKa = 11.84 RR44 pKa = 11.84 VLLVQLDD51 pKa = 3.83 LVYY54 pKa = 10.88 RR55 pKa = 11.84 LAYY58 pKa = 9.49 TPTQSLVV65 pKa = 3.06
Molecular weight: 7.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.35
ProMoST 10.116
Dawson 10.54
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.76
Grimsley 10.628
Solomon 10.613
Lehninger 10.584
Nozaki 10.394
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.76
Sillero 10.467
Patrickios 10.54
IPC_peptide 10.613
IPC2_peptide 9.487
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
9014
56
3950
693.4
77.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.534 ± 0.543
3.361 ± 0.23
5.447 ± 0.49
4.626 ± 0.476
5.458 ± 0.342
6.246 ± 0.395
1.764 ± 0.274
5.381 ± 0.417
6.091 ± 0.767
8.809 ± 0.545
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.742 ± 0.231
5.591 ± 0.815
3.772 ± 0.409
3.528 ± 0.418
3.55 ± 0.628
6.767 ± 0.674
5.957 ± 0.478
9.164 ± 0.79
1.331 ± 0.187
4.87 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here