Bos taurus papillomavirus 16
Average proteome isoelectric point is 5.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2K201|A0A1B2K201_9PAPI Minor capsid protein L2 OS=Bos taurus papillomavirus 16 OX=1887214 GN=L2 PE=3 SV=1
MM1 pKa = 7.45 HH2 pKa = 7.23 GHH4 pKa = 6.49 TPTLIDD10 pKa = 3.38 IVLEE14 pKa = 3.98 EE15 pKa = 4.95 LPDD18 pKa = 4.29 PVDD21 pKa = 3.95 LQCQEE26 pKa = 3.79 QLEE29 pKa = 4.36 NEE31 pKa = 4.26 EE32 pKa = 4.4 EE33 pKa = 4.07 EE34 pKa = 5.13 GLLLDD39 pKa = 5.01 DD40 pKa = 4.12 YY41 pKa = 11.67 VVYY44 pKa = 10.5 ACCGHH49 pKa = 6.31 CEE51 pKa = 3.89 RR52 pKa = 11.84 HH53 pKa = 5.28 LKK55 pKa = 10.24 VYY57 pKa = 10.39 IRR59 pKa = 11.84 GTVEE63 pKa = 3.75 SVRR66 pKa = 11.84 RR67 pKa = 11.84 LQEE70 pKa = 3.95 LLLSSLEE77 pKa = 4.36 FVCPACGRR85 pKa = 11.84 PRR87 pKa = 11.84 RR88 pKa = 3.9
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.465
IPC2_protein 4.749
IPC_protein 4.622
Toseland 4.507
ProMoST 4.724
Dawson 4.571
Bjellqvist 4.711
Wikipedia 4.406
Rodwell 4.482
Grimsley 4.431
Solomon 4.558
Lehninger 4.52
Nozaki 4.685
DTASelect 4.774
Thurlkill 4.507
EMBOSS 4.431
Sillero 4.749
Patrickios 2.028
IPC_peptide 4.571
IPC2_peptide 4.749
IPC2.peptide.svr19 4.675
Protein with the highest isoelectric point:
>tr|A0A1B2K253|A0A1B2K253_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 16 OX=1887214 GN=E1 PE=3 SV=1
MM1 pKa = 6.91 EE2 pKa = 4.72 TLLARR7 pKa = 11.84 LDD9 pKa = 3.83 ALQEE13 pKa = 4.01 QLLTLYY19 pKa = 10.56 EE20 pKa = 4.43 SDD22 pKa = 4.76 SKK24 pKa = 11.61 DD25 pKa = 2.85 IDD27 pKa = 3.81 DD28 pKa = 5.37 QIKK31 pKa = 9.28 HH32 pKa = 5.43 WSLLRR37 pKa = 11.84 QEE39 pKa = 5.1 YY40 pKa = 10.16 VLQHH44 pKa = 6.32 FARR47 pKa = 11.84 KK48 pKa = 9.53 HH49 pKa = 5.3 SISRR53 pKa = 11.84 LGMQTVLPLQVSQHH67 pKa = 5.4 KK68 pKa = 10.13 AKK70 pKa = 10.42 EE71 pKa = 4.16 AIEE74 pKa = 3.81 MVMYY78 pKa = 10.92 LEE80 pKa = 4.29 SLKK83 pKa = 11.07 NSTFAVEE90 pKa = 4.35 PWTLTDD96 pKa = 3.31 TSRR99 pKa = 11.84 EE100 pKa = 3.95 MFIIPPKK107 pKa = 10.1 NCFKK111 pKa = 11.04 KK112 pKa = 10.75 GGTTVDD118 pKa = 2.54 VWYY121 pKa = 10.01 DD122 pKa = 3.39 CLQANSMHH130 pKa = 5.31 YY131 pKa = 8.35 TAWSYY136 pKa = 11.17 IYY138 pKa = 10.91 YY139 pKa = 8.26 EE140 pKa = 4.64 TEE142 pKa = 3.98 SGWIKK147 pKa = 11.19 AEE149 pKa = 3.92 GQVDD153 pKa = 4.05 YY154 pKa = 11.2 NGCYY158 pKa = 10.07 YY159 pKa = 10.78 EE160 pKa = 5.33 DD161 pKa = 4.28 GNTRR165 pKa = 11.84 IYY167 pKa = 8.98 YY168 pKa = 9.28 TRR170 pKa = 11.84 FEE172 pKa = 5.33 DD173 pKa = 4.41 DD174 pKa = 3.11 AQRR177 pKa = 11.84 YY178 pKa = 7.04 SKK180 pKa = 10.97 NMEE183 pKa = 3.87 WEE185 pKa = 4.17 VHH187 pKa = 5.35 YY188 pKa = 8.82 KK189 pKa = 8.16 THH191 pKa = 5.75 VFSPVASVSSTSDD204 pKa = 3.01 SWCGGEE210 pKa = 4.22 PDD212 pKa = 3.79 STGGPLSEE220 pKa = 4.19 QHH222 pKa = 6.79 SLPLDD227 pKa = 3.75 NKK229 pKa = 8.39 TSTWRR234 pKa = 11.84 AAADD238 pKa = 3.49 RR239 pKa = 11.84 DD240 pKa = 4.2 TNNNRR245 pKa = 11.84 DD246 pKa = 3.27 IRR248 pKa = 11.84 NWSQDD253 pKa = 3.4 TVDD256 pKa = 3.54 GATVDD261 pKa = 2.92 RR262 pKa = 11.84 HH263 pKa = 4.57 TVQRR267 pKa = 11.84 ACGDD271 pKa = 3.5 HH272 pKa = 5.75 NRR274 pKa = 11.84 RR275 pKa = 11.84 GARR278 pKa = 11.84 RR279 pKa = 11.84 GRR281 pKa = 11.84 GGLQGRR287 pKa = 11.84 KK288 pKa = 6.29 RR289 pKa = 11.84 HH290 pKa = 5.95 RR291 pKa = 11.84 SRR293 pKa = 11.84 EE294 pKa = 4.05 EE295 pKa = 3.92 GGSGQEE301 pKa = 4.59 NRR303 pKa = 11.84 QQSQTSKK310 pKa = 10.32 RR311 pKa = 11.84 PCEE314 pKa = 3.36 RR315 pKa = 11.84 RR316 pKa = 11.84 LRR318 pKa = 11.84 HH319 pKa = 5.19 RR320 pKa = 11.84 QRR322 pKa = 11.84 PSGEE326 pKa = 4.09 GQPQPRR332 pKa = 11.84 PRR334 pKa = 11.84 EE335 pKa = 4.07 APAAAQQGGDD345 pKa = 3.31 TSEE348 pKa = 4.18 EE349 pKa = 4.16 EE350 pKa = 3.7 EE351 pKa = 5.81 DD352 pKa = 4.56 IRR354 pKa = 11.84 QLPQRR359 pKa = 11.84 GTPPHH364 pKa = 6.19 SPEE367 pKa = 4.06 RR368 pKa = 11.84 PVDD371 pKa = 3.61 ARR373 pKa = 11.84 GHH375 pKa = 5.84 GEE377 pKa = 3.96 GAGEE381 pKa = 3.73 PRR383 pKa = 11.84 RR384 pKa = 11.84 PRR386 pKa = 11.84 RR387 pKa = 11.84 AVRR390 pKa = 11.84 PIEE393 pKa = 4.39 HH394 pKa = 6.61 NCDD397 pKa = 3.27 TPVLLFRR404 pKa = 11.84 GGANQLKK411 pKa = 8.84 CARR414 pKa = 11.84 YY415 pKa = 8.42 RR416 pKa = 11.84 WLRR419 pKa = 11.84 QYY421 pKa = 11.65 AHH423 pKa = 7.41 LFLAISKK430 pKa = 7.5 TWGWVGAVGLGASSRR445 pKa = 11.84 LLLAFEE451 pKa = 4.74 STTQRR456 pKa = 11.84 NQFLDD461 pKa = 4.28 TVPMPPGVTLGWGRR475 pKa = 11.84 LSSLL479 pKa = 3.86
Molecular weight: 54.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.435
IPC2_protein 7.761
IPC_protein 7.658
Toseland 7.395
ProMoST 8.185
Dawson 8.317
Bjellqvist 8.668
Wikipedia 8.258
Rodwell 8.331
Grimsley 7.454
Solomon 8.419
Lehninger 8.419
Nozaki 8.799
DTASelect 8.419
Thurlkill 8.463
EMBOSS 8.536
Sillero 8.77
Patrickios 4.507
IPC_peptide 8.404
IPC2_peptide 7.732
IPC2.peptide.svr19 7.723
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2619
88
611
374.1
42.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.498 ± 0.54
2.482 ± 0.68
6.529 ± 0.464
6.758 ± 0.562
3.475 ± 0.523
6.682 ± 0.687
2.711 ± 0.307
4.582 ± 0.542
4.811 ± 0.834
7.827 ± 0.704
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.411
4.124 ± 0.519
6.606 ± 1.089
4.506 ± 0.448
6.224 ± 0.856
7.102 ± 0.912
6.796 ± 0.41
5.613 ± 0.515
1.833 ± 0.339
3.627 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here