Beijerinckia sp. 28-YEA-48
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5975 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H4KSZ1|A0A1H4KSZ1_9RHIZ Uncharacterized protein OS=Beijerinckia sp. 28-YEA-48 OX=1882748 GN=SAMN05443249_0675 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.34 VFAPAAMSLLLSAVQPASAQWAPEE26 pKa = 3.82 QGDD29 pKa = 4.17 GFSAGYY35 pKa = 9.26 FWALRR40 pKa = 11.84 NHH42 pKa = 4.46 VTEE45 pKa = 5.71 GYY47 pKa = 10.12 RR48 pKa = 11.84 CDD50 pKa = 3.85 SFDD53 pKa = 3.4 DD54 pKa = 3.89 AFRR57 pKa = 11.84 AGCNAYY63 pKa = 7.56 TQNFTSEE70 pKa = 4.65 DD71 pKa = 3.96 DD72 pKa = 3.82 EE73 pKa = 7.72 DD74 pKa = 6.39 DD75 pKa = 6.17 DD76 pKa = 6.22 GDD78 pKa = 3.73 EE79 pKa = 4.57 DD80 pKa = 4.1
Molecular weight: 8.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.465
ProMoST 3.808
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.63
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.808
Patrickios 1.875
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A1H4U120|A0A1H4U120_9RHIZ Maleylacetoacetate isomerase OS=Beijerinckia sp. 28-YEA-48 OX=1882748 GN=SAMN05443249_3136 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPSKK9 pKa = 8.8 IVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.3 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5975
0
5975
1913401
29
5085
320.2
34.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.273 ± 0.039
0.837 ± 0.01
5.433 ± 0.024
5.086 ± 0.034
3.892 ± 0.02
8.477 ± 0.051
2.026 ± 0.019
5.574 ± 0.024
3.514 ± 0.028
9.924 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.017
2.88 ± 0.034
5.27 ± 0.028
3.354 ± 0.02
6.749 ± 0.032
5.636 ± 0.036
5.522 ± 0.038
7.373 ± 0.027
1.337 ± 0.012
2.317 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here