Wuhan Mosquito Virus 9
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5KF56|A0A0B5KF56_9RHAB RNA-dependent RNA polymerase OS=Wuhan Mosquito Virus 9 OX=1608134 PE=4 SV=1
MM1 pKa = 7.44 SKK3 pKa = 10.31 ISICPVFVVPSYY15 pKa = 11.58 NFMVTFSEE23 pKa = 4.52 IIKK26 pKa = 10.43 KK27 pKa = 9.94 GAAISKK33 pKa = 9.87 QILGRR38 pKa = 11.84 IIYY41 pKa = 10.48 SNVYY45 pKa = 9.02 VDD47 pKa = 4.72 KK48 pKa = 10.63 EE49 pKa = 4.42 SEE51 pKa = 4.28 AVRR54 pKa = 11.84 SVYY57 pKa = 9.15 RR58 pKa = 11.84 TGCLLSLSYY67 pKa = 10.56 TGLSPVAWLVKK78 pKa = 9.53 AAKK81 pKa = 9.43 AKK83 pKa = 10.8 SMSQLQLLKK92 pKa = 10.62 KK93 pKa = 10.42 LYY95 pKa = 8.68 IPKK98 pKa = 9.19 MEE100 pKa = 4.3 SFITKK105 pKa = 9.99 YY106 pKa = 10.41 ISLSKK111 pKa = 10.54 ISDD114 pKa = 3.74 ATWVYY119 pKa = 10.8 CRR121 pKa = 11.84 LLSDD125 pKa = 3.62 SALLKK130 pKa = 10.65 LSASNEE136 pKa = 3.97 PLATAVFVALCYY148 pKa = 10.3 NPKK151 pKa = 10.39 APDD154 pKa = 3.36 MAVWEE159 pKa = 4.64 IPSLQCISYY168 pKa = 8.76 EE169 pKa = 4.11 DD170 pKa = 3.69 VVRR173 pKa = 11.84 AHH175 pKa = 7.28 CISEE179 pKa = 4.08 AMMEE183 pKa = 4.29 SPEE186 pKa = 4.24 LLVEE190 pKa = 4.12 QKK192 pKa = 10.62 PLTKK196 pKa = 10.11 EE197 pKa = 4.07 AEE199 pKa = 4.12 EE200 pKa = 4.17 LTQKK204 pKa = 10.88 LQTTAKK210 pKa = 9.82 NCYY213 pKa = 8.74 EE214 pKa = 3.93 KK215 pKa = 10.64 QQMVFRR221 pKa = 11.84 TLFNQNRR228 pKa = 11.84 SLLPKK233 pKa = 10.5 NVDD236 pKa = 3.89 SEE238 pKa = 4.19 DD239 pKa = 3.56 QEE241 pKa = 4.72 QIEE244 pKa = 4.45 QGDD247 pKa = 4.44 GEE249 pKa = 5.01 PGDD252 pKa = 5.92 DD253 pKa = 4.88 DD254 pKa = 4.79 DD255 pKa = 6.75 QPGQEE260 pKa = 3.91 QDD262 pKa = 3.55 AGGSTADD269 pKa = 4.01 GSDD272 pKa = 3.35 PAAGRR277 pKa = 11.84 FEE279 pKa = 4.09
Molecular weight: 30.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.916
IPC2_protein 5.029
IPC_protein 4.927
Toseland 4.774
ProMoST 4.94
Dawson 4.863
Bjellqvist 5.029
Wikipedia 4.711
Rodwell 4.762
Grimsley 4.685
Solomon 4.851
Lehninger 4.813
Nozaki 4.965
DTASelect 5.092
Thurlkill 4.774
EMBOSS 4.724
Sillero 5.029
Patrickios 3.884
IPC_peptide 4.863
IPC2_peptide 5.029
IPC2.peptide.svr19 5.025
Protein with the highest isoelectric point:
>tr|A0A0B5KTR2|A0A0B5KTR2_9RHAB Glycoprotein OS=Wuhan Mosquito Virus 9 OX=1608134 GN=G PE=4 SV=1
MM1 pKa = 7.77 EE2 pKa = 6.79 DD3 pKa = 3.36 IPMSMTLGEE12 pKa = 3.93 ARR14 pKa = 11.84 ARR16 pKa = 11.84 RR17 pKa = 11.84 AQVNVHH23 pKa = 5.97 EE24 pKa = 5.13 GEE26 pKa = 4.17 GALIGGEE33 pKa = 4.02 EE34 pKa = 4.49 FARR37 pKa = 11.84 MVGSISCTVTFAVEE51 pKa = 4.1 LDD53 pKa = 3.71 NRR55 pKa = 11.84 SHH57 pKa = 6.31 FLKK60 pKa = 10.87 LISCLIFTEE69 pKa = 4.42 SFYY72 pKa = 11.13 ATSLSYY78 pKa = 10.89 KK79 pKa = 10.17 VIQTLATGLYY89 pKa = 8.71 KK90 pKa = 10.75 VLMDD94 pKa = 4.8 PPYY97 pKa = 9.79 ATPKK101 pKa = 10.73 GSGGTQKK108 pKa = 10.04 IHH110 pKa = 5.92 VNYY113 pKa = 10.08 KK114 pKa = 9.24 FYY116 pKa = 10.97 TRR118 pKa = 11.84 TPCIRR123 pKa = 11.84 PVDD126 pKa = 4.07 LPKK129 pKa = 10.94 VGGKK133 pKa = 10.2 AIRR136 pKa = 11.84 NFSVVGNAQRR146 pKa = 11.84 CGFIEE151 pKa = 4.41 YY152 pKa = 10.3 NFSLTVHH159 pKa = 6.96 HH160 pKa = 6.95 LRR162 pKa = 11.84 ANEE165 pKa = 3.74 VGRR168 pKa = 11.84 IEE170 pKa = 3.98 GEE172 pKa = 4.02 YY173 pKa = 8.76 YY174 pKa = 10.93 KK175 pKa = 10.76 NVEE178 pKa = 4.11 EE179 pKa = 4.67 HH180 pKa = 7.23 LSDD183 pKa = 4.66 LKK185 pKa = 11.24 CKK187 pKa = 9.65 TLMKK191 pKa = 10.31 RR192 pKa = 11.84 IAEE195 pKa = 3.9 IRR197 pKa = 11.84 KK198 pKa = 9.67 DD199 pKa = 3.45 FTQFRR204 pKa = 11.84 LADD207 pKa = 3.58 YY208 pKa = 10.77 LGLLNYY214 pKa = 8.22 SHH216 pKa = 8.06 KK217 pKa = 10.55 GRR219 pKa = 11.84 QLGKK223 pKa = 9.67 IQSFPDD229 pKa = 3.14 GWLEE233 pKa = 3.82 SSLAGEE239 pKa = 4.59 PSQPKK244 pKa = 10.12 SPEE247 pKa = 3.74 PLTRR251 pKa = 11.84 GAKK254 pKa = 10.03 AMAAIKK260 pKa = 9.4 TRR262 pKa = 11.84 VWKK265 pKa = 10.65 RR266 pKa = 11.84 KK267 pKa = 9.3 GDD269 pKa = 3.46
Molecular weight: 30.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.825
IPC2_protein 8.77
IPC_protein 8.682
Toseland 9.326
ProMoST 9.151
Dawson 9.633
Bjellqvist 9.414
Wikipedia 9.809
Rodwell 9.867
Grimsley 9.706
Solomon 9.677
Lehninger 9.633
Nozaki 9.531
DTASelect 9.341
Thurlkill 9.487
EMBOSS 9.78
Sillero 9.604
Patrickios 4.863
IPC_peptide 9.677
IPC2_peptide 8.112
IPC2.peptide.svr19 7.778
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3936
269
1727
656.0
74.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.437 ± 0.569
2.109 ± 0.118
4.726 ± 0.342
6.072 ± 0.352
4.217 ± 0.286
5.31 ± 0.489
2.744 ± 0.425
6.021 ± 0.252
5.742 ± 0.447
10.722 ± 0.596
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.244
4.217 ± 0.351
5.081 ± 0.265
3.659 ± 0.386
5.285 ± 0.243
8.765 ± 0.457
5.691 ± 0.348
6.529 ± 0.448
1.804 ± 0.288
3.506 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here