Streptococcus phage IPP38
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5SC28|A0A1S5SC28_9CAUD Uncharacterized protein OS=Streptococcus phage IPP38 OX=1916177 GN=IPP38_00025 PE=4 SV=1
MM1 pKa = 7.91 AILDD5 pKa = 4.57 DD6 pKa = 4.75 LQALYY11 pKa = 11.25 DD12 pKa = 4.06 NGWDD16 pKa = 4.07 ASFNYY21 pKa = 9.53 NGQVCGIFPNSIYY34 pKa = 10.86 DD35 pKa = 3.58 VVVIIADD42 pKa = 3.53 DD43 pKa = 4.13 EE44 pKa = 4.47 YY45 pKa = 10.96 RR46 pKa = 11.84 ASSFDD51 pKa = 4.4 DD52 pKa = 4.95 LISLQIEE59 pKa = 4.74 GKK61 pKa = 7.71 TLPEE65 pKa = 3.62 IMNEE69 pKa = 3.98 VEE71 pKa = 4.11 VQYY74 pKa = 11.6 GG75 pKa = 3.21
Molecular weight: 8.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.094
IPC2_protein 3.389
IPC_protein 3.3
Toseland 3.121
ProMoST 3.541
Dawson 3.338
Bjellqvist 3.503
Wikipedia 3.325
Rodwell 3.16
Grimsley 3.045
Solomon 3.261
Lehninger 3.223
Nozaki 3.49
DTASelect 3.668
Thurlkill 3.21
EMBOSS 3.338
Sillero 3.439
Patrickios 0.006
IPC_peptide 3.261
IPC2_peptide 3.401
IPC2.peptide.svr19 3.651
Protein with the highest isoelectric point:
>tr|A0A1S5SBY4|A0A1S5SBY4_9CAUD CI-like repressor OS=Streptococcus phage IPP38 OX=1916177 GN=IPP38_00003 PE=4 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 PRR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 9.18 PYY8 pKa = 10.05 SGKK11 pKa = 10.15 KK12 pKa = 9.81 EE13 pKa = 4.19 STFVKK18 pKa = 10.4 ADD20 pKa = 3.56 PEE22 pKa = 4.22 LMLNINKK29 pKa = 9.29 IDD31 pKa = 3.66 VGNIQAKK38 pKa = 10.48 NIFGKK43 pKa = 10.24 II44 pKa = 3.35
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.084
IPC2_protein 9.414
IPC_protein 9.428
Toseland 10.262
ProMoST 9.809
Dawson 10.394
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 11.008
Grimsley 10.438
Solomon 10.423
Lehninger 10.409
Nozaki 10.204
DTASelect 9.999
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.292
Patrickios 10.833
IPC_peptide 10.438
IPC2_peptide 8.434
IPC2.peptide.svr19 8.471
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
63
0
63
12612
35
1871
200.2
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.843 ± 0.546
0.539 ± 0.101
6.224 ± 0.3
8.08 ± 0.456
4.004 ± 0.259
6.304 ± 0.525
1.522 ± 0.176
6.787 ± 0.315
8.722 ± 0.367
8.262 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.226
5.249 ± 0.311
2.743 ± 0.263
3.972 ± 0.197
4.567 ± 0.359
6.137 ± 0.327
6.129 ± 0.371
6.28 ± 0.208
1.356 ± 0.153
3.83 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here