Dickeya phage Sucellus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GLI1|A0A2S1GLI1_9CAUD Uncharacterized protein OS=Dickeya phage Sucellus OX=2163632 PE=4 SV=1
MM1 pKa = 7.23EE2 pKa = 4.6VNMFEE7 pKa = 5.19LVQCVSIVAIVVMFAALLIAAVSEE31 pKa = 4.89FIEE34 pKa = 4.28

Molecular weight: 3.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GLD8|A0A2S1GLD8_9CAUD Uncharacterized protein OS=Dickeya phage Sucellus OX=2163632 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.55GKK4 pKa = 8.54LTNVSGISIQTKK16 pKa = 10.15NSIIDD21 pKa = 3.07ICEE24 pKa = 3.74AKK26 pKa = 10.15FANSTTWPANLSGASGFYY44 pKa = 10.96AMYY47 pKa = 11.06AEE49 pKa = 5.5FYY51 pKa = 10.07NHH53 pKa = 5.68NTVVDD58 pKa = 4.56SILYY62 pKa = 9.12LGPVKK67 pKa = 9.49PTARR71 pKa = 11.84QIRR74 pKa = 11.84RR75 pKa = 11.84MKK77 pKa = 9.99RR78 pKa = 11.84RR79 pKa = 11.84LRR81 pKa = 11.84RR82 pKa = 11.84GQQ84 pKa = 3.09

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12415

34

1135

225.7

25.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.839 ± 0.397

1.474 ± 0.216

5.928 ± 0.216

6.242 ± 0.222

4.108 ± 0.245

7.628 ± 0.35

1.482 ± 0.174

7.652 ± 0.303

6.75 ± 0.363

6.83 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.173

6.307 ± 0.436

3.182 ± 0.273

3.335 ± 0.438

4.503 ± 0.245

8.055 ± 0.346

5.356 ± 0.382

6.444 ± 0.294

1.16 ± 0.092

3.729 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski