Streptococcus satellite phage Javan344
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQK7|A0A4D5ZQK7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan344 OX=2558652 GN=JavanS344_0006 PE=4 SV=1
MM1 pKa = 7.7 IIDD4 pKa = 3.31 GRR6 pKa = 11.84 EE7 pKa = 3.86 YY8 pKa = 10.44 VHH10 pKa = 6.98 CPVCGTVTAVYY21 pKa = 9.26 DD22 pKa = 3.65 ICDD25 pKa = 3.28 VCQWQNTGEE34 pKa = 4.15 TNIDD38 pKa = 3.44 GGPNEE43 pKa = 4.17 MTLAEE48 pKa = 4.26 AKK50 pKa = 9.24 EE51 pKa = 4.27 AYY53 pKa = 10.0 VKK55 pKa = 10.91 GITIII60 pKa = 3.7
Molecular weight: 6.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.081
IPC2_protein 4.266
IPC_protein 4.075
Toseland 3.91
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.923
Grimsley 3.834
Solomon 4.024
Lehninger 3.973
Nozaki 4.177
DTASelect 4.317
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.19
Patrickios 0.223
IPC_peptide 4.024
IPC2_peptide 4.177
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A4D5ZK83|A0A4D5ZK83_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan344 OX=2558652 GN=JavanS344_0011 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.54 KK7 pKa = 10.41 KK8 pKa = 10.18 KK9 pKa = 10.4 DD10 pKa = 3.2 GSVVYY15 pKa = 10.36 RR16 pKa = 11.84 SSIYY20 pKa = 10.7 LGIDD24 pKa = 2.74 SMTGKK29 pKa = 10.07 KK30 pKa = 10.27 VKK32 pKa = 8.2 TTISARR38 pKa = 11.84 TKK40 pKa = 10.54 RR41 pKa = 11.84 EE42 pKa = 4.09 LNSKK46 pKa = 10.11 AIQAKK51 pKa = 9.87 VEE53 pKa = 4.13 FEE55 pKa = 4.42 KK56 pKa = 10.89 KK57 pKa = 10.63 GSTVCSAVEE66 pKa = 3.56 IKK68 pKa = 9.81 TYY70 pKa = 11.24 AEE72 pKa = 4.37 LVEE75 pKa = 4.62 NWLEE79 pKa = 4.57 TYY81 pKa = 10.41 CHH83 pKa = 5.52 TVKK86 pKa = 10.74 KK87 pKa = 9.48 STLTITKK94 pKa = 9.59 VRR96 pKa = 11.84 INNYY100 pKa = 9.58 LLPAFGDD107 pKa = 3.76 YY108 pKa = 10.88 KK109 pKa = 10.73 LDD111 pKa = 3.6 KK112 pKa = 9.52 LTPPVIQKK120 pKa = 9.14 QVNQWAKK127 pKa = 10.93 DD128 pKa = 3.66 YY129 pKa = 10.39 NQLGKK134 pKa = 10.73 GYY136 pKa = 9.95 QEE138 pKa = 3.81 YY139 pKa = 8.41 TQLNSLNKK147 pKa = 10.13 RR148 pKa = 11.84 ILKK151 pKa = 9.76 YY152 pKa = 10.38 AVSLQVISFNPARR165 pKa = 11.84 DD166 pKa = 3.33 IIVPRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.78 EE174 pKa = 3.83 KK175 pKa = 10.94 EE176 pKa = 4.02 EE177 pKa = 3.89 QKK179 pKa = 10.87 LKK181 pKa = 11.19 YY182 pKa = 10.41 LDD184 pKa = 4.45 DD185 pKa = 4.4 EE186 pKa = 4.34 NLKK189 pKa = 10.8 KK190 pKa = 10.47 FLAYY194 pKa = 10.4 LDD196 pKa = 4.13 QLPNTYY202 pKa = 10.75 KK203 pKa = 10.74 NFYY206 pKa = 9.48 DD207 pKa = 3.51 TVLYY211 pKa = 8.15 KK212 pKa = 10.45 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.08 CLALKK228 pKa = 10.08 WSDD231 pKa = 2.92 IDD233 pKa = 4.61 LKK235 pKa = 11.41 NGTLDD240 pKa = 3.34 VNKK243 pKa = 9.41 TLNCEE248 pKa = 4.23 KK249 pKa = 10.6 EE250 pKa = 3.72 ITTPKK255 pKa = 8.66 TKK257 pKa = 10.57 SSVRR261 pKa = 11.84 VIDD264 pKa = 4.38 LDD266 pKa = 3.78 NKK268 pKa = 8.09 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.7 KK277 pKa = 10.34 NRR279 pKa = 11.84 QAQVGRR285 pKa = 11.84 EE286 pKa = 3.53 IGLTYY291 pKa = 10.73 EE292 pKa = 4.43 KK293 pKa = 10.84 VFPNYY298 pKa = 9.73 FDD300 pKa = 3.77 EE301 pKa = 4.53 YY302 pKa = 10.53 RR303 pKa = 11.84 DD304 pKa = 3.64 ARR306 pKa = 11.84 ALRR309 pKa = 11.84 PRR311 pKa = 11.84 LEE313 pKa = 3.98 KK314 pKa = 10.35 HH315 pKa = 6.18 LKK317 pKa = 9.98 LSKK320 pKa = 10.39 CPRR323 pKa = 11.84 LTFHH327 pKa = 7.71 AFRR330 pKa = 11.84 HH331 pKa = 4.54 THH333 pKa = 6.71 ASILLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 5.05 SQISITMDD361 pKa = 3.56 TYY363 pKa = 11.78 SHH365 pKa = 7.28 LSKK368 pKa = 10.83 DD369 pKa = 3.71 NKK371 pKa = 9.08 KK372 pKa = 7.72 TATSFYY378 pKa = 9.98 EE379 pKa = 3.93 KK380 pKa = 10.3 AIEE383 pKa = 4.04 KK384 pKa = 10.35 LKK386 pKa = 11.16 SSS388 pKa = 3.82
Molecular weight: 45.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.072
IPC2_protein 9.136
IPC_protein 8.99
Toseland 9.794
ProMoST 9.487
Dawson 10.014
Bjellqvist 9.677
Wikipedia 10.175
Rodwell 10.496
Grimsley 10.087
Solomon 10.028
Lehninger 9.999
Nozaki 9.809
DTASelect 9.663
Thurlkill 9.867
EMBOSS 10.204
Sillero 9.94
Patrickios 9.355
IPC_peptide 10.028
IPC2_peptide 8.2
IPC2.peptide.svr19 8.091
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3589
39
484
156.0
18.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.102 ± 0.42
0.892 ± 0.195
6.548 ± 0.419
9.39 ± 0.644
4.765 ± 0.442
3.929 ± 0.318
1.783 ± 0.148
6.771 ± 0.63
10.226 ± 0.512
9.111 ± 0.504
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.25
5.628 ± 0.328
2.368 ± 0.239
3.817 ± 0.365
5.043 ± 0.431
5.099 ± 0.443
5.266 ± 0.453
5.545 ± 0.38
0.892 ± 0.147
4.542 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here