Phasianus colchicus (Common pheasant)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A669Q1U1|A0A669Q1U1_PHACC SPARC (osteonectin) cwcv and kazal like domains proteoglycan 1 OS=Phasianus colchicus OX=9054 PE=4 SV=1
MM1 pKa = 7.43SSSLGRR7 pKa = 11.84STTWVRR13 pKa = 11.84PSRR16 pKa = 11.84APCPGPAMPPGTQSSARR33 pKa = 11.84SAALSRR39 pKa = 11.84DD40 pKa = 3.53GAGDD44 pKa = 3.49PGMWIPQGQALPAAGEE60 pKa = 4.29GAAEE64 pKa = 3.96QAGAGARR71 pKa = 11.84EE72 pKa = 4.47GWTGVMMMVVMVGVMVGMVMVGGDD96 pKa = 4.14DD97 pKa = 5.0DD98 pKa = 6.47DD99 pKa = 7.27DD100 pKa = 6.09GFDD103 pKa = 4.05SDD105 pKa = 5.77GGGDD109 pKa = 4.32DD110 pKa = 5.32DD111 pKa = 5.71DD112 pKa = 6.31GSGGSVVDD120 pKa = 4.82GDD122 pKa = 5.66DD123 pKa = 3.56VDD125 pKa = 6.0DD126 pKa = 6.08DD127 pKa = 5.11GGDD130 pKa = 3.54VDD132 pKa = 6.37DD133 pKa = 6.36GDD135 pKa = 6.01DD136 pKa = 5.0GDD138 pKa = 5.52DD139 pKa = 3.86VDD141 pKa = 6.6DD142 pKa = 6.71GGDD145 pKa = 3.69DD146 pKa = 4.46DD147 pKa = 6.07NDD149 pKa = 4.11GGGDD153 pKa = 3.91DD154 pKa = 5.86DD155 pKa = 6.54GGGDD159 pKa = 5.68DD160 pKa = 6.6DD161 pKa = 4.91GTLQNASGSATDD173 pKa = 4.14ASASEE178 pKa = 4.48AFWNEE183 pKa = 3.31QVLGVTHH190 pKa = 6.98SSGLGDD196 pKa = 3.93PGSVQWLLALCLLAAWLVVFLCMLRR221 pKa = 11.84GIHH224 pKa = 6.69SSGKK228 pKa = 9.38VVYY231 pKa = 8.85ITATFPYY238 pKa = 10.21LILLILIIRR247 pKa = 11.84GATLPGSLDD256 pKa = 3.18GVRR259 pKa = 11.84FYY261 pKa = 11.48LSSDD265 pKa = 2.67WSKK268 pKa = 11.28LLSAQVWSDD277 pKa = 2.9AASQIFYY284 pKa = 10.98SLGIGFGGLLSMASYY299 pKa = 11.56NKK301 pKa = 9.66FDD303 pKa = 4.22NNVVRR308 pKa = 11.84DD309 pKa = 3.88TLVIAVGNCCTSFFAGFAIFSVLGHH334 pKa = 6.21MALKK338 pKa = 10.34RR339 pKa = 11.84DD340 pKa = 3.54IPVGSVAEE348 pKa = 4.35SGPGLAFVAYY358 pKa = 9.43PEE360 pKa = 4.58ALSLLPGSPFWSILFFLMLFMLGVDD385 pKa = 3.52TLFGNIEE392 pKa = 4.85AITTAIMDD400 pKa = 4.18EE401 pKa = 4.63FPALRR406 pKa = 11.84EE407 pKa = 4.02GRR409 pKa = 11.84RR410 pKa = 11.84KK411 pKa = 10.47VMLLAVLCFSFFLLGLLLVTQGGIFWFTLIDD442 pKa = 3.63TYY444 pKa = 11.09STGFGLIIITLLMCIGIASCYY465 pKa = 10.0GIEE468 pKa = 4.24RR469 pKa = 11.84FCRR472 pKa = 11.84DD473 pKa = 3.74IVTMICLCPPWYY485 pKa = 10.42SRR487 pKa = 11.84VLGCFKK493 pKa = 10.68VCWVFFTPCLLLFTLIFTFLDD514 pKa = 3.77MYY516 pKa = 10.68NIPLSYY522 pKa = 9.43GTYY525 pKa = 10.12EE526 pKa = 4.1FPAWGTSLGVCMGVLSCVQIPIGAIVALCHH556 pKa = 5.06QTGTLTDD563 pKa = 4.07RR564 pKa = 11.84FQKK567 pKa = 10.68AMQPLHH573 pKa = 6.0SWGTAAASAEE583 pKa = 4.48DD584 pKa = 3.94IIEE587 pKa = 4.23VPFTITLSSSDD598 pKa = 3.82FTTPPHH604 pKa = 6.6SEE606 pKa = 3.97SS607 pKa = 3.4

Molecular weight: 64.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A669QY88|A0A669QY88_PHACC Cyclin L2 OS=Phasianus colchicus OX=9054 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15705

9802

25507

17908591

25

8758

702.1

78.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.837 ± 0.015

2.302 ± 0.015

4.974 ± 0.01

7.202 ± 0.019

3.7 ± 0.01

6.194 ± 0.018

2.529 ± 0.007

4.758 ± 0.011

6.052 ± 0.017

9.684 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.006

3.972 ± 0.01

5.608 ± 0.019

4.721 ± 0.014

5.448 ± 0.014

8.324 ± 0.017

5.308 ± 0.01

6.121 ± 0.01

1.201 ± 0.005

2.792 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski