Tenacibaculum phage JQ
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XUV2|A0A6G6XUV2_9CAUD Uncharacterized protein OS=Tenacibaculum phage JQ OX=2712935 PE=4 SV=1
MM1 pKa = 7.79 ASNIIIQFNSALQSGDD17 pKa = 3.47 FLRR20 pKa = 11.84 LQDD23 pKa = 3.74 SYY25 pKa = 11.37 TPNVEE30 pKa = 3.83 MGFYY34 pKa = 9.29 WGSASGFLPVTGNISSDD51 pKa = 2.83 INNLKK56 pKa = 10.31 NRR58 pKa = 11.84 INTAYY63 pKa = 10.65 NSTNRR68 pKa = 11.84 YY69 pKa = 6.18 TVTSDD74 pKa = 2.79 ATSITITDD82 pKa = 3.74 NIGFSEE88 pKa = 4.51 FSEE91 pKa = 4.66 VNNSTGARR99 pKa = 11.84 LDD101 pKa = 3.27 ITIVNDD107 pKa = 3.44 SQQIGVNIDD116 pKa = 3.55 NIEE119 pKa = 4.43 LIEE122 pKa = 4.34 NATNPCDD129 pKa = 3.68 LVDD132 pKa = 4.51 VRR134 pKa = 11.84 ITTNEE139 pKa = 3.68 APFDD143 pKa = 3.94 LLQPVSVTDD152 pKa = 3.59 IQTTTYY158 pKa = 9.81 TISDD162 pKa = 3.77 VQRR165 pKa = 11.84 SINFINVEE173 pKa = 4.18 VKK175 pKa = 10.4 KK176 pKa = 11.29 GNITDD181 pKa = 3.97 LEE183 pKa = 4.46 EE184 pKa = 4.39 IFAPVINSEE193 pKa = 4.17 LFKK196 pKa = 10.92 LDD198 pKa = 3.41 VVNTPSGATLSIRR211 pKa = 11.84 WKK213 pKa = 10.19 GVRR216 pKa = 11.84 NPFFDD221 pKa = 4.63 LEE223 pKa = 4.1 YY224 pKa = 10.51 SINGVDD230 pKa = 4.16 YY231 pKa = 11.03 YY232 pKa = 11.51 SSTSFSGLEE241 pKa = 3.95 DD242 pKa = 3.45 GNYY245 pKa = 9.02 TLYY248 pKa = 10.4 IRR250 pKa = 11.84 DD251 pKa = 4.27 SIGCSITIPFEE262 pKa = 4.0 VTEE265 pKa = 4.39 FEE267 pKa = 4.67 PNVYY271 pKa = 10.11 EE272 pKa = 3.77 RR273 pKa = 11.84 TSFFEE278 pKa = 4.04 ISEE281 pKa = 4.2 QNSNIWIKK289 pKa = 10.15 QEE291 pKa = 4.05 YY292 pKa = 9.71 KK293 pKa = 10.67 DD294 pKa = 3.66 SCNVYY299 pKa = 10.9 SNLTNTLSYY308 pKa = 11.58 EE309 pKa = 3.89 EE310 pKa = 4.2 TTQVNNQSFKK320 pKa = 11.19 QLFQKK325 pKa = 10.77 CDD327 pKa = 3.91 GIVTNQYY334 pKa = 7.53 KK335 pKa = 9.73 TNYY338 pKa = 8.73 EE339 pKa = 3.87 NVEE342 pKa = 4.08 IKK344 pKa = 10.89 LIDD347 pKa = 3.81 CDD349 pKa = 4.21 GNEE352 pKa = 4.22 SLLVSNQISQNINITDD368 pKa = 3.47 VRR370 pKa = 11.84 DD371 pKa = 3.78 VTIEE375 pKa = 3.78 AYY377 pKa = 9.64 EE378 pKa = 4.01 YY379 pKa = 10.94 NEE381 pKa = 4.1 SSFVGVTYY389 pKa = 10.93 VSGNTYY395 pKa = 11.03 DD396 pKa = 4.72 SNTLTANGSYY406 pKa = 10.57 YY407 pKa = 10.53 LASSVPSFMNIDD419 pKa = 3.71 DD420 pKa = 5.53 FIQIEE425 pKa = 4.29 GAGWYY430 pKa = 9.21 RR431 pKa = 11.84 VRR433 pKa = 11.84 DD434 pKa = 3.5 IIFYY438 pKa = 11.28 NNIQTLILDD447 pKa = 3.82 VLEE450 pKa = 4.56 SDD452 pKa = 5.25 FPISLNQTVKK462 pKa = 9.73 GTSIYY467 pKa = 10.45 NQLDD471 pKa = 3.66 YY472 pKa = 11.28 EE473 pKa = 4.51 LYY475 pKa = 9.39 EE476 pKa = 4.06 ASYY479 pKa = 11.39 DD480 pKa = 3.94 LSTLNGDD487 pKa = 3.75 YY488 pKa = 11.0 YY489 pKa = 10.92 LTLDD493 pKa = 3.74 ATDD496 pKa = 4.2 SEE498 pKa = 5.07 FDD500 pKa = 3.61 TVSYY504 pKa = 10.19 RR505 pKa = 11.84 SEE507 pKa = 3.94 WFNVASMQYY516 pKa = 8.5 NTYY519 pKa = 10.72 LLQYY523 pKa = 9.93 YY524 pKa = 10.19 NSEE527 pKa = 4.25 NNEE530 pKa = 4.01 TNYY533 pKa = 11.03 SNGIRR538 pKa = 11.84 NKK540 pKa = 10.1 FRR542 pKa = 11.84 IRR544 pKa = 11.84 YY545 pKa = 7.38 QKK547 pKa = 10.33 QFTYY551 pKa = 10.4 IPNDD555 pKa = 3.47 TNEE558 pKa = 4.12 VYY560 pKa = 9.5 LTDD563 pKa = 3.75 TNAVQTDD570 pKa = 3.55 SQYY573 pKa = 11.38 RR574 pKa = 11.84 DD575 pKa = 3.69 YY576 pKa = 11.66 LKK578 pKa = 10.74 LDD580 pKa = 4.15 LMPMPVNMVRR590 pKa = 11.84 KK591 pKa = 10.15 LGLAFSNDD599 pKa = 2.74 RR600 pKa = 11.84 VFINGLSLIKK610 pKa = 10.82 NNEE613 pKa = 4.05 LEE615 pKa = 4.06 VEE617 pKa = 4.53 RR618 pKa = 11.84 IGEE621 pKa = 4.16 SNEE624 pKa = 3.66 YY625 pKa = 10.03 RR626 pKa = 11.84 FSAQFVRR633 pKa = 11.84 SDD635 pKa = 3.31 YY636 pKa = 11.53 AFDD639 pKa = 3.54 VTISDD644 pKa = 3.7 NSIVLPQGQPLQINDD659 pKa = 3.52 NAEE662 pKa = 3.74 GLLFINN668 pKa = 5.04
Molecular weight: 76.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.843
IPC2_protein 4.024
IPC_protein 4.024
Toseland 3.821
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.897
Rodwell 3.859
Grimsley 3.732
Solomon 3.999
Lehninger 3.948
Nozaki 4.101
DTASelect 4.317
Thurlkill 3.859
EMBOSS 3.91
Sillero 4.139
Patrickios 0.769
IPC_peptide 3.999
IPC2_peptide 4.126
IPC2.peptide.svr19 4.023
Protein with the highest isoelectric point:
>tr|A0A6G6XUY7|A0A6G6XUY7_9CAUD Uncharacterized protein OS=Tenacibaculum phage JQ OX=2712935 PE=4 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 LIVVNRR8 pKa = 11.84 RR9 pKa = 11.84 YY10 pKa = 10.46 LLRR13 pKa = 11.84 GKK15 pKa = 9.5 FFRR18 pKa = 11.84 YY19 pKa = 9.96 NRR21 pKa = 11.84 MKK23 pKa = 10.58 GVKK26 pKa = 9.97 DD27 pKa = 3.67 LGFKK31 pKa = 10.11 FAIGIYY37 pKa = 7.61 EE38 pKa = 3.77 FRR40 pKa = 11.84 FYY42 pKa = 11.21 RR43 pKa = 4.1
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 10.423
IPC_protein 11.359
Toseland 11.359
ProMoST 11.828
Dawson 11.403
Bjellqvist 11.316
Wikipedia 11.798
Rodwell 11.389
Grimsley 11.462
Solomon 11.784
Lehninger 11.696
Nozaki 11.359
DTASelect 11.316
Thurlkill 11.359
EMBOSS 11.828
Sillero 11.374
Patrickios 11.14
IPC_peptide 11.784
IPC2_peptide 10.701
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
11129
42
755
154.6
17.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.502 ± 0.334
1.339 ± 0.201
6.092 ± 0.208
7.674 ± 0.33
4.421 ± 0.292
4.996 ± 0.315
1.465 ± 0.199
7.898 ± 0.275
10.154 ± 0.871
8.536 ± 0.318
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.13 ± 0.161
7.79 ± 0.356
2.309 ± 0.151
3.271 ± 0.195
4.035 ± 0.272
5.895 ± 0.387
5.661 ± 0.314
5.571 ± 0.269
1.195 ± 0.12
5.068 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here