Potato virus T (isolate Chenopodium amaranticolor/Peru/-/1992) (PVT)
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5ACE4|B5ACE4_PVT92 Coat protein OS=Potato virus T (isolate Chenopodium amaranticolor/Peru/-/1992) OX=1289386 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 4.56 PTTFVQIRR10 pKa = 11.84 DD11 pKa = 3.57 EE12 pKa = 4.23 VLNLTVAAYY21 pKa = 10.15 SSQWDD26 pKa = 3.96 GQATQALKK34 pKa = 10.91 DD35 pKa = 3.66 GAKK38 pKa = 9.95 EE39 pKa = 3.64 QMLRR43 pKa = 11.84 FLFGRR48 pKa = 11.84 IAISSASRR56 pKa = 11.84 NTIWPDD62 pKa = 3.19 TEE64 pKa = 4.04 IASEE68 pKa = 3.98 DD69 pKa = 3.66 LQIGMSAASAGPPPVAAAPISLIFRR94 pKa = 11.84 VNFNSYY100 pKa = 9.59 VKK102 pKa = 10.24 MLIALSNTSTNSFVKK117 pKa = 10.64 NKK119 pKa = 7.38 TLRR122 pKa = 11.84 QMCMPFAKK130 pKa = 10.12 YY131 pKa = 10.18 AYY133 pKa = 9.77 GYY135 pKa = 9.57 LSEE138 pKa = 4.53 MGYY141 pKa = 8.36 ATWAYY146 pKa = 9.99 EE147 pKa = 4.66 KK148 pKa = 10.22 MPKK151 pKa = 9.97 LCRR154 pKa = 11.84 KK155 pKa = 9.06 AKK157 pKa = 9.02 WVAFDD162 pKa = 4.03 FASGLLIDD170 pKa = 4.32 TTMQLNDD177 pKa = 3.88 DD178 pKa = 4.63 EE179 pKa = 4.7 KK180 pKa = 10.76 TVIQGLGARR189 pKa = 11.84 LFKK192 pKa = 9.87 TQQSIQIADD201 pKa = 3.37 STMDD205 pKa = 3.24 GEE207 pKa = 4.83 AINRR211 pKa = 11.84 EE212 pKa = 3.94 II213 pKa = 5.08
Molecular weight: 23.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.823
IPC2_protein 6.122
IPC_protein 6.351
Toseland 5.982
ProMoST 6.478
Dawson 6.605
Bjellqvist 6.326
Wikipedia 6.504
Rodwell 6.517
Grimsley 5.881
Solomon 6.693
Lehninger 6.693
Nozaki 6.517
DTASelect 6.737
Thurlkill 6.561
EMBOSS 6.678
Sillero 6.825
Patrickios 4.355
IPC_peptide 6.707
IPC2_peptide 6.3
IPC2.peptide.svr19 6.426
Protein with the highest isoelectric point:
>tr|B5ACE4|B5ACE4_PVT92 Coat protein OS=Potato virus T (isolate Chenopodium amaranticolor/Peru/-/1992) OX=1289386 PE=4 SV=1
MM1 pKa = 7.41 EE2 pKa = 6.3 LISVEE7 pKa = 3.93 KK8 pKa = 10.46 FRR10 pKa = 11.84 RR11 pKa = 11.84 QWEE14 pKa = 4.09 EE15 pKa = 3.92 RR16 pKa = 11.84 EE17 pKa = 4.56 SITGPVDD24 pKa = 2.97 SGAIYY29 pKa = 10.29 TNSAFHH35 pKa = 6.05 NLKK38 pKa = 9.39 TKK40 pKa = 7.48 WHH42 pKa = 6.03 VYY44 pKa = 10.09 KK45 pKa = 10.59 SEE47 pKa = 4.06 CSIGLDD53 pKa = 3.85 LPDD56 pKa = 3.73 NGKK59 pKa = 10.11 IISKK63 pKa = 9.9 DD64 pKa = 2.93 IPLFDD69 pKa = 4.49 QEE71 pKa = 4.73 EE72 pKa = 4.2 IDD74 pKa = 6.11 NIMKK78 pKa = 10.12 DD79 pKa = 3.45 DD80 pKa = 3.77 KK81 pKa = 11.28 QVFVHH86 pKa = 6.67 LGAFVFGLVAHH97 pKa = 6.48 FPVDD101 pKa = 3.7 EE102 pKa = 4.2 EE103 pKa = 4.85 VEE105 pKa = 4.28 GLVSIIDD112 pKa = 3.54 KK113 pKa = 10.64 RR114 pKa = 11.84 RR115 pKa = 11.84 TDD117 pKa = 3.39 LRR119 pKa = 11.84 RR120 pKa = 11.84 ATLACRR126 pKa = 11.84 KK127 pKa = 9.38 IKK129 pKa = 10.17 FVNGRR134 pKa = 11.84 CAFMMKK140 pKa = 10.13 PNFSVRR146 pKa = 11.84 KK147 pKa = 8.93 EE148 pKa = 3.88 DD149 pKa = 4.97 LRR151 pKa = 11.84 DD152 pKa = 3.4 GDD154 pKa = 4.32 TFCAAIKK161 pKa = 10.15 IKK163 pKa = 10.83 NLGFEE168 pKa = 4.24 GGFFPFSACGGVIYY182 pKa = 8.71 RR183 pKa = 11.84 TSNVSFAHH191 pKa = 6.6 AVDD194 pKa = 3.33 KK195 pKa = 10.78 TFASRR200 pKa = 11.84 TVHH203 pKa = 6.88 DD204 pKa = 4.99 LVGTDD209 pKa = 4.36 ILSLDD214 pKa = 3.57 QLDD217 pKa = 3.94 RR218 pKa = 11.84 ATLEE222 pKa = 3.99 DD223 pKa = 3.88 LEE225 pKa = 4.45 EE226 pKa = 4.2 VRR228 pKa = 11.84 RR229 pKa = 11.84 SPILRR234 pKa = 11.84 LTAPDD239 pKa = 3.59 EE240 pKa = 4.0 RR241 pKa = 11.84 VMIEE245 pKa = 3.76 RR246 pKa = 11.84 GNWFQKK252 pKa = 10.3 KK253 pKa = 8.03 PAIRR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 SFGKK263 pKa = 10.06 RR264 pKa = 11.84 RR265 pKa = 11.84 PQKK268 pKa = 9.68 SSSVRR273 pKa = 11.84 SASLPRR279 pKa = 11.84 FSCSEE284 pKa = 3.56 RR285 pKa = 11.84 LEE287 pKa = 4.3 RR288 pKa = 11.84 GFEE291 pKa = 4.12 SEE293 pKa = 4.27 SLAGLVLDD301 pKa = 5.05 QKK303 pKa = 11.12 YY304 pKa = 10.49 GPNNFCADD312 pKa = 3.29 KK313 pKa = 10.65 RR314 pKa = 11.84 RR315 pKa = 11.84 SPEE318 pKa = 3.72 SDD320 pKa = 3.14 SGSLQQSMGRR330 pKa = 11.84 SSHH333 pKa = 6.28 SGSEE337 pKa = 4.08 GWGKK341 pKa = 8.5 GTDD344 pKa = 3.34 VAIPLRR350 pKa = 11.84 KK351 pKa = 9.35 NRR353 pKa = 11.84 NIFSEE358 pKa = 4.3
Molecular weight: 40.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.983
IPC2_protein 8.083
IPC_protein 7.98
Toseland 8.141
ProMoST 8.551
Dawson 8.843
Bjellqvist 8.99
Wikipedia 8.814
Rodwell 8.96
Grimsley 7.966
Solomon 8.99
Lehninger 8.99
Nozaki 9.311
DTASelect 8.77
Thurlkill 8.916
EMBOSS 9.063
Sillero 9.18
Patrickios 5.27
IPC_peptide 8.99
IPC2_peptide 7.746
IPC2.peptide.svr19 7.775
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2177
213
1606
725.7
82.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.293 ± 1.386
1.791 ± 0.217
6.798 ± 0.296
7.304 ± 0.849
6.615 ± 0.528
5.374 ± 0.792
2.021 ± 0.537
5.926 ± 0.288
7.625 ± 0.723
9.371 ± 0.934
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.389 ± 0.608
3.95 ± 0.138
3.491 ± 0.206
2.848 ± 0.709
6.293 ± 1.233
7.671 ± 0.918
4.593 ± 0.674
5.926 ± 0.433
1.378 ± 0.15
2.343 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here