Dorea sp. CAG:317

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; environmental samples

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6XNN9|R6XNN9_9FIRM 50S ribosomal protein L4 OS=Dorea sp. CAG:317 OX=1262873 GN=rplD PE=3 SV=1
MM1 pKa = 7.82KK2 pKa = 10.34KK3 pKa = 10.11RR4 pKa = 11.84LLSVLLCAGLIASLAVGCGGGKK26 pKa = 9.87DD27 pKa = 3.29AGKK30 pKa = 10.35DD31 pKa = 3.7GEE33 pKa = 4.62KK34 pKa = 9.44EE35 pKa = 4.28TLSVWLPPLDD45 pKa = 5.46DD46 pKa = 3.89EE47 pKa = 4.85TEE49 pKa = 4.08KK50 pKa = 11.33NYY52 pKa = 10.22VPLLDD57 pKa = 4.79KK58 pKa = 11.22FEE60 pKa = 4.61EE61 pKa = 4.39EE62 pKa = 4.46NNCEE66 pKa = 4.45LDD68 pKa = 3.74VQIIPWDD75 pKa = 3.85TYY77 pKa = 8.08EE78 pKa = 4.13EE79 pKa = 4.83KK80 pKa = 11.3YY81 pKa = 7.38MTGINADD88 pKa = 3.62EE89 pKa = 4.99GPDD92 pKa = 3.38VGYY95 pKa = 9.25MYY97 pKa = 11.51VEE99 pKa = 4.26MFPTYY104 pKa = 9.21IDD106 pKa = 3.25SGAVVDD112 pKa = 3.96MSEE115 pKa = 4.09YY116 pKa = 10.95LSDD119 pKa = 3.66EE120 pKa = 4.51DD121 pKa = 4.15YY122 pKa = 11.7EE123 pKa = 4.46EE124 pKa = 4.25YY125 pKa = 10.83LYY127 pKa = 11.07LDD129 pKa = 4.18KK130 pKa = 11.68GEE132 pKa = 4.3MMGGQYY138 pKa = 10.33GVPIVTGNPFIMYY151 pKa = 9.11YY152 pKa = 10.57NKK154 pKa = 10.61DD155 pKa = 3.23ILDD158 pKa = 3.93EE159 pKa = 4.69LGEE162 pKa = 4.29KK163 pKa = 10.51APEE166 pKa = 3.61TWEE169 pKa = 3.89DD170 pKa = 3.63FKK172 pKa = 11.39RR173 pKa = 11.84ICEE176 pKa = 4.1KK177 pKa = 10.1ATKK180 pKa = 9.36DD181 pKa = 3.1TDD183 pKa = 3.62GDD185 pKa = 4.12GKK187 pKa = 10.26IDD189 pKa = 3.5QYY191 pKa = 11.91GYY193 pKa = 11.25AAGFNNGDD201 pKa = 3.58MSPLYY206 pKa = 10.56LLNSYY211 pKa = 10.29YY212 pKa = 10.89YY213 pKa = 10.76SLLWQSGSDD222 pKa = 4.1IYY224 pKa = 11.57NDD226 pKa = 3.55DD227 pKa = 3.89LKK229 pKa = 11.14SVRR232 pKa = 11.84FNDD235 pKa = 3.45EE236 pKa = 3.95AGVKK240 pKa = 9.59AVEE243 pKa = 4.2YY244 pKa = 10.22LKK246 pKa = 11.05SLTPYY251 pKa = 9.71MPEE254 pKa = 4.72DD255 pKa = 4.21YY256 pKa = 9.92MSLAATDD263 pKa = 3.82AFSSVFGGGKK273 pKa = 9.75AAFACARR280 pKa = 11.84AMQAQTEE287 pKa = 4.76SFKK290 pKa = 10.39EE291 pKa = 4.34TYY293 pKa = 10.08PDD295 pKa = 4.15LNWDD299 pKa = 3.71YY300 pKa = 10.35VTSLKK305 pKa = 10.83GEE307 pKa = 4.0QYY309 pKa = 9.87GTFGAADD316 pKa = 4.37CLTLMSACEE325 pKa = 3.92NKK327 pKa = 10.06EE328 pKa = 3.93LGMKK332 pKa = 9.9LIKK335 pKa = 10.8YY336 pKa = 7.36MVGSEE341 pKa = 4.44VMTAYY346 pKa = 10.4HH347 pKa = 6.68KK348 pKa = 10.48EE349 pKa = 4.03HH350 pKa = 6.88FGAPMTADD358 pKa = 3.24EE359 pKa = 5.43PYY361 pKa = 10.67QGDD364 pKa = 3.72EE365 pKa = 3.84KK366 pKa = 11.23LEE368 pKa = 4.67RR369 pKa = 11.84ILTEE373 pKa = 5.86DD374 pKa = 3.39RR375 pKa = 11.84DD376 pKa = 3.43KK377 pKa = 10.95WRR379 pKa = 11.84PLQAGPCGSDD389 pKa = 2.48ILLNLTSQLQAVMSGDD405 pKa = 3.81AEE407 pKa = 4.16AKK409 pKa = 8.99EE410 pKa = 4.39ALDD413 pKa = 3.79EE414 pKa = 4.38AADD417 pKa = 3.91YY418 pKa = 11.53SNEE421 pKa = 4.04LLDD424 pKa = 5.26EE425 pKa = 4.14YY426 pKa = 10.53WADD429 pKa = 3.72KK430 pKa = 10.38EE431 pKa = 4.21

Molecular weight: 48.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6XJZ0|R6XJZ0_9FIRM Tripeptide aminopeptidase OS=Dorea sp. CAG:317 OX=1262873 GN=BN605_02204 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2206

0

2206

683009

29

3000

309.6

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.377 ± 0.047

1.48 ± 0.023

5.472 ± 0.043

8.31 ± 0.071

3.975 ± 0.038

7.249 ± 0.048

1.785 ± 0.021

7.387 ± 0.049

7.25 ± 0.042

8.976 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.243 ± 0.03

4.08 ± 0.036

3.151 ± 0.024

3.156 ± 0.03

4.29 ± 0.038

5.414 ± 0.038

5.277 ± 0.046

7.221 ± 0.043

0.82 ± 0.019

4.085 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski