Amino acid dipepetide frequency for Methylophilales bacterium HTCC2181

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.404AlaAla: 5.404 ± 0.162
0.739AlaCys: 0.739 ± 0.042
3.524AlaAsp: 3.524 ± 0.102
4.239AlaGlu: 4.239 ± 0.113
3.112AlaPhe: 3.112 ± 0.087
4.95AlaGly: 4.95 ± 0.177
1.399AlaHis: 1.399 ± 0.057
6.549AlaIle: 6.549 ± 0.15
5.231AlaLys: 5.231 ± 0.155
7.729AlaLeu: 7.729 ± 0.154
1.989AlaMet: 1.989 ± 0.071
3.351AlaAsn: 3.351 ± 0.096
2.181AlaPro: 2.181 ± 0.072
2.357AlaGln: 2.357 ± 0.077
2.779AlaArg: 2.779 ± 0.086
5.011AlaSer: 5.011 ± 0.13
3.515AlaThr: 3.515 ± 0.108
4.124AlaVal: 4.124 ± 0.106
0.736AlaTrp: 0.736 ± 0.045
2.074AlaTyr: 2.074 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.604CysAla: 0.604 ± 0.04
0.093CysCys: 0.093 ± 0.017
0.553CysAsp: 0.553 ± 0.039
0.526CysGlu: 0.526 ± 0.037
0.436CysPhe: 0.436 ± 0.031
0.846CysGly: 0.846 ± 0.05
0.3CysHis: 0.3 ± 0.027
0.685CysIle: 0.685 ± 0.045
0.544CysLys: 0.544 ± 0.035
0.882CysLeu: 0.882 ± 0.047
0.193CysMet: 0.193 ± 0.021
0.456CysAsn: 0.456 ± 0.035
0.502CysPro: 0.502 ± 0.034
0.288CysGln: 0.288 ± 0.025
0.331CysArg: 0.331 ± 0.029
0.7CysSer: 0.7 ± 0.041
0.424CysThr: 0.424 ± 0.03
0.551CysVal: 0.551 ± 0.034
0.08CysTrp: 0.08 ± 0.014
0.266CysTyr: 0.266 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
3.775AspAla: 3.775 ± 0.117
0.558AspCys: 0.558 ± 0.04
2.92AspAsp: 2.92 ± 0.089
3.795AspGlu: 3.795 ± 0.116
2.788AspPhe: 2.788 ± 0.088
3.79AspGly: 3.79 ± 0.113
1.204AspHis: 1.204 ± 0.052
4.938AspIle: 4.938 ± 0.1
3.666AspLys: 3.666 ± 0.108
5.874AspLeu: 5.874 ± 0.115
1.455AspMet: 1.455 ± 0.057
2.693AspAsn: 2.693 ± 0.082
2.228AspPro: 2.228 ± 0.077
2.147AspGln: 2.147 ± 0.087
2.308AspArg: 2.308 ± 0.085
3.312AspSer: 3.312 ± 0.099
2.418AspThr: 2.418 ± 0.076
3.388AspVal: 3.388 ± 0.103
0.665AspTrp: 0.665 ± 0.042
2.028AspTyr: 2.028 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
4.619GluAla: 4.619 ± 0.146
0.468GluCys: 0.468 ± 0.032
3.405GluAsp: 3.405 ± 0.099
4.078GluGlu: 4.078 ± 0.114
2.515GluPhe: 2.515 ± 0.081
3.775GluGly: 3.775 ± 0.115
1.311GluHis: 1.311 ± 0.055
5.474GluIle: 5.474 ± 0.116
5.703GluLys: 5.703 ± 0.127
5.942GluLeu: 5.942 ± 0.123
1.579GluMet: 1.579 ± 0.066
3.266GluAsn: 3.266 ± 0.096
1.709GluPro: 1.709 ± 0.075
1.955GluGln: 1.955 ± 0.068
2.627GluArg: 2.627 ± 0.083
4.212GluSer: 4.212 ± 0.093
2.774GluThr: 2.774 ± 0.087
4.083GluVal: 4.083 ± 0.115
0.58GluTrp: 0.58 ± 0.037
1.733GluTyr: 1.733 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
2.849PheAla: 2.849 ± 0.084
0.461PheCys: 0.461 ± 0.033
2.954PheAsp: 2.954 ± 0.092
2.51PheGlu: 2.51 ± 0.081
2.457PhePhe: 2.457 ± 0.103
3.176PheGly: 3.176 ± 0.095
0.904PheHis: 0.904 ± 0.048
4.034PheIle: 4.034 ± 0.121
2.888PheLys: 2.888 ± 0.08
4.221PheLeu: 4.221 ± 0.129
1.111PheMet: 1.111 ± 0.055
2.818PheAsn: 2.818 ± 0.105
1.616PhePro: 1.616 ± 0.066
1.277PheGln: 1.277 ± 0.058
1.521PheArg: 1.521 ± 0.06
3.736PheSer: 3.736 ± 0.097
2.315PheThr: 2.315 ± 0.077
2.593PheVal: 2.593 ± 0.083
0.461PheTrp: 0.461 ± 0.039
1.709PheTyr: 1.709 ± 0.072
0.0PheXaa: 0.0 ± 0.0
Gly
4.794GlyAla: 4.794 ± 0.166
0.787GlyCys: 0.787 ± 0.054
3.481GlyAsp: 3.481 ± 0.103
3.783GlyGlu: 3.783 ± 0.095
3.339GlyPhe: 3.339 ± 0.088
5.17GlyGly: 5.17 ± 0.478
1.543GlyHis: 1.543 ± 0.066
5.862GlyIle: 5.862 ± 0.119
4.626GlyLys: 4.626 ± 0.122
6.893GlyLeu: 6.893 ± 0.122
1.913GlyMet: 1.913 ± 0.066
2.905GlyAsn: 2.905 ± 0.129
1.726GlyPro: 1.726 ± 0.064
2.198GlyGln: 2.198 ± 0.089
2.771GlyArg: 2.771 ± 0.1
4.655GlySer: 4.655 ± 0.23
3.307GlyThr: 3.307 ± 0.137
4.446GlyVal: 4.446 ± 0.113
0.846GlyTrp: 0.846 ± 0.051
2.374GlyTyr: 2.374 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.567HisAla: 1.567 ± 0.067
0.28HisCys: 0.28 ± 0.03
1.126HisAsp: 1.126 ± 0.054
1.187HisGlu: 1.187 ± 0.054
0.977HisPhe: 0.977 ± 0.049
1.54HisGly: 1.54 ± 0.062
0.707HisHis: 0.707 ± 0.041
1.682HisIle: 1.682 ± 0.062
1.353HisLys: 1.353 ± 0.05
2.089HisLeu: 2.089 ± 0.074
0.48HisMet: 0.48 ± 0.034
1.026HisAsn: 1.026 ± 0.05
1.111HisPro: 1.111 ± 0.055
0.88HisGln: 0.88 ± 0.049
0.773HisArg: 0.773 ± 0.043
1.316HisSer: 1.316 ± 0.057
1.055HisThr: 1.055 ± 0.055
1.153HisVal: 1.153 ± 0.059
0.207HisTrp: 0.207 ± 0.023
0.802HisTyr: 0.802 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.425IleAla: 6.425 ± 0.14
0.7IleCys: 0.7 ± 0.038
5.735IleAsp: 5.735 ± 0.132
5.818IleGlu: 5.818 ± 0.104
3.812IlePhe: 3.812 ± 0.118
5.659IleGly: 5.659 ± 0.127
1.757IleHis: 1.757 ± 0.071
6.771IleIle: 6.771 ± 0.154
7.21IleLys: 7.21 ± 0.15
7.322IleLeu: 7.322 ± 0.163
1.901IleMet: 1.901 ± 0.065
5.165IleAsn: 5.165 ± 0.126
3.442IlePro: 3.442 ± 0.086
2.805IleGln: 2.805 ± 0.092
3.144IleArg: 3.144 ± 0.089
6.052IleSer: 6.052 ± 0.141
4.726IleThr: 4.726 ± 0.115
4.897IleVal: 4.897 ± 0.12
0.536IleTrp: 0.536 ± 0.036
2.508IleTyr: 2.508 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
5.248LysAla: 5.248 ± 0.134
0.431LysCys: 0.431 ± 0.034
4.229LysAsp: 4.229 ± 0.116
5.16LysGlu: 5.16 ± 0.15
2.491LysPhe: 2.491 ± 0.08
4.136LysGly: 4.136 ± 0.114
1.423LysHis: 1.423 ± 0.065
7.034LysIle: 7.034 ± 0.144
7.748LysLys: 7.748 ± 0.219
6.466LysLeu: 6.466 ± 0.118
1.789LysMet: 1.789 ± 0.073
5.182LysAsn: 5.182 ± 0.12
2.652LysPro: 2.652 ± 0.106
2.469LysGln: 2.469 ± 0.077
2.818LysArg: 2.818 ± 0.104
4.702LysSer: 4.702 ± 0.109
3.853LysThr: 3.853 ± 0.099
4.326LysVal: 4.326 ± 0.112
0.578LysTrp: 0.578 ± 0.035
2.108LysTyr: 2.108 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
7.351LeuAla: 7.351 ± 0.147
0.948LeuCys: 0.948 ± 0.049
5.296LeuAsp: 5.296 ± 0.131
5.847LeuGlu: 5.847 ± 0.131
4.421LeuPhe: 4.421 ± 0.125
6.566LeuGly: 6.566 ± 0.156
1.789LeuHis: 1.789 ± 0.062
8.47LeuIle: 8.47 ± 0.162
7.046LeuLys: 7.046 ± 0.143
9.311LeuLeu: 9.311 ± 0.208
2.662LeuMet: 2.662 ± 0.092
5.35LeuAsn: 5.35 ± 0.115
3.929LeuPro: 3.929 ± 0.103
2.744LeuGln: 2.744 ± 0.082
3.797LeuArg: 3.797 ± 0.103
7.797LeuSer: 7.797 ± 0.176
5.333LeuThr: 5.333 ± 0.137
5.74LeuVal: 5.74 ± 0.136
0.895LeuTrp: 0.895 ± 0.055
2.552LeuTyr: 2.552 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
2.106MetAla: 2.106 ± 0.084
0.144MetCys: 0.144 ± 0.019
1.343MetAsp: 1.343 ± 0.062
1.419MetGlu: 1.419 ± 0.054
0.955MetPhe: 0.955 ± 0.054
1.847MetGly: 1.847 ± 0.074
0.59MetHis: 0.59 ± 0.045
2.018MetIle: 2.018 ± 0.071
1.779MetLys: 1.779 ± 0.062
2.513MetLeu: 2.513 ± 0.082
0.724MetMet: 0.724 ± 0.048
1.377MetAsn: 1.377 ± 0.063
1.075MetPro: 1.075 ± 0.056
1.094MetGln: 1.094 ± 0.054
1.133MetArg: 1.133 ± 0.049
1.767MetSer: 1.767 ± 0.062
1.45MetThr: 1.45 ± 0.062
1.609MetVal: 1.609 ± 0.065
0.188MetTrp: 0.188 ± 0.02
0.478MetTyr: 0.478 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.583AsnAla: 3.583 ± 0.101
0.478AsnCys: 0.478 ± 0.04
2.976AsnAsp: 2.976 ± 0.096
3.078AsnGlu: 3.078 ± 0.084
2.481AsnPhe: 2.481 ± 0.08
3.156AsnGly: 3.156 ± 0.139
1.265AsnHis: 1.265 ± 0.062
5.104AsnIle: 5.104 ± 0.107
4.204AsnLys: 4.204 ± 0.114
5.048AsnLeu: 5.048 ± 0.122
1.238AsnMet: 1.238 ± 0.053
3.349AsnAsn: 3.349 ± 0.109
2.491AsnPro: 2.491 ± 0.076
2.201AsnGln: 2.201 ± 0.075
1.972AsnArg: 1.972 ± 0.074
3.086AsnSer: 3.086 ± 0.115
2.569AsnThr: 2.569 ± 0.102
2.896AsnVal: 2.896 ± 0.088
0.522AsnTrp: 0.522 ± 0.037
1.762AsnTyr: 1.762 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
2.264ProAla: 2.264 ± 0.101
0.334ProCys: 0.334 ± 0.03
2.169ProAsp: 2.169 ± 0.079
2.666ProGlu: 2.666 ± 0.095
1.757ProPhe: 1.757 ± 0.069
2.411ProGly: 2.411 ± 0.079
0.778ProHis: 0.778 ± 0.046
3.1ProIle: 3.1 ± 0.091
2.652ProLys: 2.652 ± 0.082
3.556ProLeu: 3.556 ± 0.1
1.029ProMet: 1.029 ± 0.054
2.011ProAsn: 2.011 ± 0.072
1.114ProPro: 1.114 ± 0.054
1.146ProGln: 1.146 ± 0.056
1.221ProArg: 1.221 ± 0.063
2.693ProSer: 2.693 ± 0.085
1.882ProThr: 1.882 ± 0.074
2.389ProVal: 2.389 ± 0.077
0.356ProTrp: 0.356 ± 0.031
1.216ProTyr: 1.216 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
2.532GlnAla: 2.532 ± 0.082
0.349GlnCys: 0.349 ± 0.031
1.726GlnAsp: 1.726 ± 0.067
2.152GlnGlu: 2.152 ± 0.074
1.64GlnPhe: 1.64 ± 0.057
2.106GlnGly: 2.106 ± 0.078
0.778GlnHis: 0.778 ± 0.044
2.786GlnIle: 2.786 ± 0.079
2.659GlnLys: 2.659 ± 0.081
3.434GlnLeu: 3.434 ± 0.104
0.838GlnMet: 0.838 ± 0.042
1.54GlnAsn: 1.54 ± 0.068
1.182GlnPro: 1.182 ± 0.048
1.197GlnGln: 1.197 ± 0.063
1.345GlnArg: 1.345 ± 0.059
2.471GlnSer: 2.471 ± 0.081
1.601GlnThr: 1.601 ± 0.068
1.989GlnVal: 1.989 ± 0.063
0.461GlnTrp: 0.461 ± 0.04
1.094GlnTyr: 1.094 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.601ArgAla: 2.601 ± 0.071
0.327ArgCys: 0.327 ± 0.029
2.108ArgAsp: 2.108 ± 0.081
2.506ArgGlu: 2.506 ± 0.092
1.945ArgPhe: 1.945 ± 0.083
2.437ArgGly: 2.437 ± 0.089
0.802ArgHis: 0.802 ± 0.046
3.071ArgIle: 3.071 ± 0.1
2.693ArgLys: 2.693 ± 0.09
4.075ArgLeu: 4.075 ± 0.096
1.126ArgMet: 1.126 ± 0.056
1.882ArgAsn: 1.882 ± 0.069
1.438ArgPro: 1.438 ± 0.067
1.297ArgGln: 1.297 ± 0.065
1.65ArgArg: 1.65 ± 0.074
2.547ArgSer: 2.547 ± 0.068
1.796ArgThr: 1.796 ± 0.071
2.666ArgVal: 2.666 ± 0.087
0.363ArgTrp: 0.363 ± 0.03
1.331ArgTyr: 1.331 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
4.526SerAla: 4.526 ± 0.121
0.643SerCys: 0.643 ± 0.04
3.751SerAsp: 3.751 ± 0.099
3.927SerGlu: 3.927 ± 0.11
3.393SerPhe: 3.393 ± 0.093
4.955SerGly: 4.955 ± 0.242
1.501SerHis: 1.501 ± 0.062
6.288SerIle: 6.288 ± 0.138
4.616SerLys: 4.616 ± 0.118
7.341SerLeu: 7.341 ± 0.156
1.879SerMet: 1.879 ± 0.065
3.766SerAsn: 3.766 ± 0.106
2.401SerPro: 2.401 ± 0.075
2.557SerGln: 2.557 ± 0.083
2.581SerArg: 2.581 ± 0.078
5.243SerSer: 5.243 ± 0.141
3.247SerThr: 3.247 ± 0.104
4.039SerVal: 4.039 ± 0.09
0.778SerTrp: 0.778 ± 0.045
2.172SerTyr: 2.172 ± 0.077
0.0SerXaa: 0.0 ± 0.0
Thr
3.32ThrAla: 3.32 ± 0.11
0.409ThrCys: 0.409 ± 0.033
2.681ThrAsp: 2.681 ± 0.077
2.752ThrGlu: 2.752 ± 0.082
2.274ThrPhe: 2.274 ± 0.077
3.595ThrGly: 3.595 ± 0.154
1.228ThrHis: 1.228 ± 0.056
4.358ThrIle: 4.358 ± 0.103
3.668ThrLys: 3.668 ± 0.107
5.235ThrLeu: 5.235 ± 0.174
1.136ThrMet: 1.136 ± 0.057
2.615ThrAsn: 2.615 ± 0.088
2.281ThrPro: 2.281 ± 0.081
1.84ThrGln: 1.84 ± 0.06
1.655ThrArg: 1.655 ± 0.067
3.346ThrSer: 3.346 ± 0.129
2.562ThrThr: 2.562 ± 0.124
3.11ThrVal: 3.11 ± 0.097
0.363ThrTrp: 0.363 ± 0.029
1.531ThrTyr: 1.531 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
4.531ValAla: 4.531 ± 0.12
0.651ValCys: 0.651 ± 0.034
3.79ValAsp: 3.79 ± 0.089
3.775ValGlu: 3.775 ± 0.098
2.574ValPhe: 2.574 ± 0.101
4.426ValGly: 4.426 ± 0.118
1.121ValHis: 1.121 ± 0.056
5.321ValIle: 5.321 ± 0.113
4.024ValLys: 4.024 ± 0.127
5.55ValLeu: 5.55 ± 0.12
1.767ValMet: 1.767 ± 0.062
2.937ValAsn: 2.937 ± 0.085
2.089ValPro: 2.089 ± 0.077
1.599ValGln: 1.599 ± 0.068
2.381ValArg: 2.381 ± 0.083
4.28ValSer: 4.28 ± 0.106
3.364ValThr: 3.364 ± 0.113
4.058ValVal: 4.058 ± 0.103
0.646ValTrp: 0.646 ± 0.05
1.575ValTyr: 1.575 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.641TrpAla: 0.641 ± 0.044
0.11TrpCys: 0.11 ± 0.016
0.485TrpAsp: 0.485 ± 0.04
0.563TrpGlu: 0.563 ± 0.04
0.556TrpPhe: 0.556 ± 0.041
0.622TrpGly: 0.622 ± 0.039
0.246TrpHis: 0.246 ± 0.025
0.802TrpIle: 0.802 ± 0.047
0.597TrpLys: 0.597 ± 0.042
1.221TrpLeu: 1.221 ± 0.054
0.241TrpMet: 0.241 ± 0.027
0.439TrpAsn: 0.439 ± 0.039
0.305TrpPro: 0.305 ± 0.032
0.412TrpGln: 0.412 ± 0.032
0.473TrpArg: 0.473 ± 0.037
0.595TrpSer: 0.595 ± 0.041
0.39TrpThr: 0.39 ± 0.031
0.673TrpVal: 0.673 ± 0.037
0.107TrpTrp: 0.107 ± 0.017
0.271TrpTyr: 0.271 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.252TyrAla: 2.252 ± 0.071
0.351TyrCys: 0.351 ± 0.032
1.699TyrAsp: 1.699 ± 0.068
2.028TyrGlu: 2.028 ± 0.076
1.687TyrPhe: 1.687 ± 0.067
2.255TyrGly: 2.255 ± 0.074
0.665TyrHis: 0.665 ± 0.041
2.038TyrIle: 2.038 ± 0.071
1.938TyrLys: 1.938 ± 0.084
3.142TyrLeu: 3.142 ± 0.1
0.556TyrMet: 0.556 ± 0.039
1.331TyrAsn: 1.331 ± 0.05
1.272TyrPro: 1.272 ± 0.054
1.419TyrGln: 1.419 ± 0.061
1.38TyrArg: 1.38 ± 0.059
2.081TyrSer: 2.081 ± 0.082
1.38TyrThr: 1.38 ± 0.058
1.731TyrVal: 1.731 ± 0.064
0.38TyrTrp: 0.38 ± 0.03
0.873TyrTyr: 0.873 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1336 proteins (410286 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski