Torque teno midi virus 2
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4GZA1|A4GZA1_9VIRU Capsid protein OS=Torque teno midi virus 2 OX=687380 PE=3 SV=1
MM1 pKa = 7.24 QNISYY6 pKa = 10.24 TDD8 pKa = 4.05 FYY10 pKa = 11.48 KK11 pKa = 9.67 PTPYY15 pKa = 9.58 NTDD18 pKa = 2.73 TKK20 pKa = 9.85 NQIWLSQLADD30 pKa = 4.2 SHH32 pKa = 7.36 DD33 pKa = 3.57 NWCNCDD39 pKa = 3.79 FPFAHH44 pKa = 7.26 LLASIFPPGHH54 pKa = 6.98 KK55 pKa = 10.31 DD56 pKa = 2.73 RR57 pKa = 11.84 TRR59 pKa = 11.84 TINQILQRR67 pKa = 11.84 DD68 pKa = 4.31 FRR70 pKa = 11.84 QKK72 pKa = 10.5 CLSGGADD79 pKa = 3.34 AKK81 pKa = 11.19 DD82 pKa = 3.29 SGLAGEE88 pKa = 5.2 DD89 pKa = 4.13 LEE91 pKa = 6.61 KK92 pKa = 10.99 DD93 pKa = 3.61 GQKK96 pKa = 10.98 DD97 pKa = 3.15 GGKK100 pKa = 8.49 YY101 pKa = 8.52 TEE103 pKa = 4.52 EE104 pKa = 4.18 DD105 pKa = 3.47 LPRR108 pKa = 11.84 DD109 pKa = 3.52 EE110 pKa = 5.63 VEE112 pKa = 4.12 EE113 pKa = 3.85 LLAAVEE119 pKa = 4.64 SAASRR124 pKa = 3.62
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.681
IPC2_protein 4.698
IPC_protein 4.609
Toseland 4.457
ProMoST 4.749
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.507
Rodwell 4.457
Grimsley 4.368
Solomon 4.584
Lehninger 4.546
Nozaki 4.698
DTASelect 4.927
Thurlkill 4.469
EMBOSS 4.52
Sillero 4.749
Patrickios 3.923
IPC_peptide 4.584
IPC2_peptide 4.736
IPC2.peptide.svr19 4.69
Protein with the highest isoelectric point:
>tr|A4GZA1|A4GZA1_9VIRU Capsid protein OS=Torque teno midi virus 2 OX=687380 PE=3 SV=1
MM1 pKa = 7.35 PFWWGRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 10.22 RR10 pKa = 11.84 FWLGRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 FRR19 pKa = 11.84 KK20 pKa = 8.78 RR21 pKa = 11.84 WPKK24 pKa = 9.9 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 KK28 pKa = 7.51 IHH30 pKa = 6.02 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 FTKK36 pKa = 10.08 RR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 RR41 pKa = 11.84 TTRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 9.21 RR49 pKa = 11.84 RR50 pKa = 11.84 FKK52 pKa = 10.79 VRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 9.51 KK57 pKa = 10.47 PKK59 pKa = 9.15 IPIYY63 pKa = 9.73 QWQPDD68 pKa = 4.33 SIRR71 pKa = 11.84 KK72 pKa = 9.16 CKK74 pKa = 10.08 IKK76 pKa = 10.8 GVGTLVLGAHH86 pKa = 7.12 GKK88 pKa = 9.59 QFVCYY93 pKa = 9.31 TDD95 pKa = 3.25 VEE97 pKa = 4.81 TRR99 pKa = 11.84 APPPKK104 pKa = 10.39 APGGGGFGCKK114 pKa = 8.44 QFSLQYY120 pKa = 10.42 LYY122 pKa = 11.14 EE123 pKa = 4.19 EE124 pKa = 4.13 YY125 pKa = 10.63 RR126 pKa = 11.84 FRR128 pKa = 11.84 NNIWTHH134 pKa = 4.39 TNINLDD140 pKa = 3.4 LVRR143 pKa = 11.84 YY144 pKa = 9.23 LRR146 pKa = 11.84 AAFTFYY152 pKa = 10.65 RR153 pKa = 11.84 HH154 pKa = 6.56 PDD156 pKa = 2.51 IDD158 pKa = 4.5 FIINYY163 pKa = 8.94 DD164 pKa = 3.87 RR165 pKa = 11.84 QPPFYY170 pKa = 9.82 LDD172 pKa = 2.95 KK173 pKa = 10.33 FTYY176 pKa = 9.35 PLCHH180 pKa = 6.4 PQNLLLGKK188 pKa = 9.87 HH189 pKa = 6.05 KK190 pKa = 10.53 IILLSKK196 pKa = 10.09 ASKK199 pKa = 10.57 PNGKK203 pKa = 8.95 VKK205 pKa = 10.38 KK206 pKa = 10.1 RR207 pKa = 11.84 IIIKK211 pKa = 9.68 PPKK214 pKa = 10.17 QMITKK219 pKa = 9.29 WFFQEE224 pKa = 4.3 QFTTQPLLSLRR235 pKa = 11.84 AAAASFQYY243 pKa = 10.07 PHH245 pKa = 7.26 IGCCMPNRR253 pKa = 11.84 VVTFSALNPGFYY265 pKa = 9.96 QQGNWSLAQQEE276 pKa = 4.41 THH278 pKa = 7.24 PYY280 pKa = 9.62 SPWPQIPRR288 pKa = 11.84 RR289 pKa = 11.84 LIFWDD294 pKa = 4.25 VPASEE299 pKa = 5.15 IPTDD303 pKa = 3.65 GQQRR307 pKa = 11.84 LDD309 pKa = 3.62 FSKK312 pKa = 10.44 KK313 pKa = 9.27 HH314 pKa = 6.0 AFITDD319 pKa = 2.9 IHH321 pKa = 7.14 SYY323 pKa = 10.26 SDD325 pKa = 3.3 SVSYY329 pKa = 11.04 NKK331 pKa = 10.6 GYY333 pKa = 7.92 FTSKK337 pKa = 9.62 ILKK340 pKa = 10.07 AKK342 pKa = 9.93 FVSKK346 pKa = 10.49 DD347 pKa = 3.05 ISDD350 pKa = 3.57 TAGIGNLPLNLCRR363 pKa = 11.84 YY364 pKa = 9.41 NPAIDD369 pKa = 3.47 TGKK372 pKa = 11.15 GNTIWLHH379 pKa = 5.47 SNLVNSYY386 pKa = 10.82 SKK388 pKa = 10.02 PQHH391 pKa = 6.82 DD392 pKa = 4.37 EE393 pKa = 4.51 DD394 pKa = 5.81 IIITGMPIWMLLFGWLSYY412 pKa = 9.29 VQHH415 pKa = 6.05 VKK417 pKa = 10.58 KK418 pKa = 10.54 PPDD421 pKa = 3.78 FYY423 pKa = 11.6 LSFTVLIEE431 pKa = 4.17 SPAIEE436 pKa = 4.31 VASASATPTPVIPIDD451 pKa = 3.38 TSFINGNPPYY461 pKa = 9.97 NQTITIRR468 pKa = 11.84 DD469 pKa = 3.72 KK470 pKa = 10.86 AHH472 pKa = 6.61 WWPDD476 pKa = 3.13 VYY478 pKa = 11.49 NQTEE482 pKa = 4.3 VLNSLVTAGPYY493 pKa = 9.33 VPKK496 pKa = 10.35 LDD498 pKa = 4.49 NIRR501 pKa = 11.84 NSTWEE506 pKa = 3.39 LHH508 pKa = 5.22 YY509 pKa = 10.39 FYY511 pKa = 11.31 NFLFKK516 pKa = 10.25 WGGPEE521 pKa = 4.17 STDD524 pKa = 3.6 QPVADD529 pKa = 4.87 PSKK532 pKa = 10.81 QPIYY536 pKa = 10.62 DD537 pKa = 4.08 ALDD540 pKa = 3.56 KK541 pKa = 10.79 QQQTVQIRR549 pKa = 11.84 NPAKK553 pKa = 10.04 QKK555 pKa = 10.26 YY556 pKa = 7.21 ATIMHH561 pKa = 6.99 PWDD564 pKa = 3.42 VRR566 pKa = 11.84 RR567 pKa = 11.84 GIITNKK573 pKa = 9.42 ALKK576 pKa = 10.28 RR577 pKa = 11.84 MYY579 pKa = 10.2 EE580 pKa = 3.98 NLSIDD585 pKa = 3.6 STFEE589 pKa = 3.92 ADD591 pKa = 3.95 SIASKK596 pKa = 10.34 RR597 pKa = 11.84 KK598 pKa = 9.41 RR599 pKa = 11.84 VTGPCFTALQEE610 pKa = 4.23 INQEE614 pKa = 3.93 EE615 pKa = 4.71 RR616 pKa = 11.84 TCLQTLCEE624 pKa = 4.0 EE625 pKa = 4.89 NIFQEE630 pKa = 4.8 TPQEE634 pKa = 4.0 EE635 pKa = 4.37 NLQQLILQQQQHH647 pKa = 4.92 QQDD650 pKa = 3.12 IKK652 pKa = 11.17 YY653 pKa = 10.33 NILKK657 pKa = 10.27 LISQMKK663 pKa = 8.29 EE664 pKa = 3.57 QQNMLKK670 pKa = 10.46 LHH672 pKa = 6.43 TGLLNN677 pKa = 3.66
Molecular weight: 79.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.199
IPC2_protein 9.428
IPC_protein 9.458
Toseland 10.101
ProMoST 9.78
Dawson 10.292
Bjellqvist 9.97
Wikipedia 10.467
Rodwell 10.73
Grimsley 10.365
Solomon 10.321
Lehninger 10.277
Nozaki 10.116
DTASelect 9.955
Thurlkill 10.145
EMBOSS 10.511
Sillero 10.218
Patrickios 10.277
IPC_peptide 10.321
IPC2_peptide 8.682
IPC2.peptide.svr19 8.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1310
124
677
327.5
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.906
1.603 ± 0.171
5.802 ± 1.275
4.58 ± 0.862
4.427 ± 0.585
4.885 ± 0.3
2.748 ± 0.209
5.878 ± 1.201
8.015 ± 0.556
8.092 ± 0.287
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.45 ± 0.2
5.344 ± 0.29
7.023 ± 0.674
6.489 ± 0.577
6.489 ± 0.63
6.794 ± 1.265
6.412 ± 0.302
2.824 ± 0.645
1.908 ± 0.458
3.511 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here