Acartia tonsa copepod circovirus
Average proteome isoelectric point is 8.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7P669|L7P669_9CIRC Uncharacterized protein OS=Acartia tonsa copepod circovirus OX=1168547 GN=AtCopCV_gp2 PE=4 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.02 SLHH5 pKa = 5.86 ISKK8 pKa = 9.92 IEE10 pKa = 4.0 FSQWPQSIKK19 pKa = 10.3 PRR21 pKa = 11.84 AKK23 pKa = 8.94 RR24 pKa = 11.84 AVGIFSLTKK33 pKa = 8.98 MPSNATTNRR42 pKa = 11.84 ARR44 pKa = 11.84 GWCFTLNNPPPEE56 pKa = 4.67 MISEE60 pKa = 4.09 RR61 pKa = 11.84 RR62 pKa = 11.84 VVVPGHH68 pKa = 6.01 VSGLDD73 pKa = 3.2 AAIPTEE79 pKa = 4.28 PNLPEE84 pKa = 5.13 HH85 pKa = 6.38 RR86 pKa = 11.84 HH87 pKa = 4.79 CDD89 pKa = 3.52 YY90 pKa = 11.05 RR91 pKa = 11.84 VGQLEE96 pKa = 4.03 RR97 pKa = 11.84 GDD99 pKa = 4.0 NGTLHH104 pKa = 5.65 IQGYY108 pKa = 5.68 YY109 pKa = 8.27 HH110 pKa = 6.51 HH111 pKa = 7.39 RR112 pKa = 11.84 VMKK115 pKa = 10.57 SFNQMKK121 pKa = 10.32 KK122 pKa = 10.43 LIPNAHH128 pKa = 6.01 WEE130 pKa = 4.1 IAKK133 pKa = 10.33 GSPKK137 pKa = 10.04 QNRR140 pKa = 11.84 DD141 pKa = 3.05 YY142 pKa = 10.41 CTKK145 pKa = 10.44 EE146 pKa = 3.87 DD147 pKa = 3.52 TRR149 pKa = 11.84 VPDD152 pKa = 3.4 TRR154 pKa = 11.84 PFEE157 pKa = 4.68 EE158 pKa = 5.6 GEE160 pKa = 4.41 CPHH163 pKa = 6.26 QGTRR167 pKa = 11.84 FDD169 pKa = 4.79 LRR171 pKa = 11.84 QCADD175 pKa = 3.03 VLANGGSLLDD185 pKa = 4.25 LPGEE189 pKa = 4.18 MVIRR193 pKa = 11.84 YY194 pKa = 8.52 HH195 pKa = 7.01 RR196 pKa = 11.84 GFSAYY201 pKa = 9.77 RR202 pKa = 11.84 SLFHH206 pKa = 7.24 KK207 pKa = 10.28 RR208 pKa = 11.84 RR209 pKa = 11.84 QEE211 pKa = 3.83 APTVLWIYY219 pKa = 10.88 GPTGSGKK226 pKa = 10.32 SRR228 pKa = 11.84 FASTLRR234 pKa = 11.84 DD235 pKa = 3.14 EE236 pKa = 5.96 CYY238 pKa = 9.39 WKK240 pKa = 10.35 PPGKK244 pKa = 9.7 WFDD247 pKa = 4.35 GYY249 pKa = 10.87 DD250 pKa = 3.52 QEE252 pKa = 4.96 PLVILDD258 pKa = 4.51 DD259 pKa = 4.32 YY260 pKa = 10.82 RR261 pKa = 11.84 SEE263 pKa = 3.8 WAEE266 pKa = 3.6 WGVFGQLLRR275 pKa = 11.84 LLDD278 pKa = 4.61 RR279 pKa = 11.84 YY280 pKa = 10.45 PLMVEE285 pKa = 4.04 YY286 pKa = 10.56 KK287 pKa = 9.53 GGSVQFCSEE296 pKa = 5.04 LIVITCDD303 pKa = 3.4 RR304 pKa = 11.84 PPSEE308 pKa = 4.64 LWFSQGDD315 pKa = 3.24 IGQLTRR321 pKa = 11.84 RR322 pKa = 11.84 ISHH325 pKa = 6.55 VIHH328 pKa = 6.75 AVGMWLPVEE337 pKa = 4.26 PAVHH341 pKa = 6.15 EE342 pKa = 4.64 SIKK345 pKa = 10.78 RR346 pKa = 11.84 RR347 pKa = 11.84 DD348 pKa = 3.68 NIEE351 pKa = 3.54
Molecular weight: 40.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.684
IPC2_protein 7.922
IPC_protein 7.805
Toseland 7.556
ProMoST 8.317
Dawson 8.507
Bjellqvist 8.829
Wikipedia 8.448
Rodwell 8.521
Grimsley 7.585
Solomon 8.609
Lehninger 8.609
Nozaki 9.004
DTASelect 8.565
Thurlkill 8.624
EMBOSS 8.712
Sillero 8.931
Patrickios 4.507
IPC_peptide 8.595
IPC2_peptide 7.834
IPC2.peptide.svr19 7.854
Protein with the highest isoelectric point:
>tr|L7P669|L7P669_9CIRC Uncharacterized protein OS=Acartia tonsa copepod circovirus OX=1168547 GN=AtCopCV_gp2 PE=4 SV=1
MM1 pKa = 7.85 PNPHH5 pKa = 6.69 FLEE8 pKa = 4.54 PLNQAFNRR16 pKa = 11.84 AAYY19 pKa = 10.03 RR20 pKa = 11.84 LLRR23 pKa = 11.84 QAGGAATGAATTALYY38 pKa = 10.35 EE39 pKa = 4.27 YY40 pKa = 10.1 ARR42 pKa = 11.84 SRR44 pKa = 11.84 ARR46 pKa = 11.84 QMAYY50 pKa = 10.29 GKK52 pKa = 10.18 RR53 pKa = 11.84 SYY55 pKa = 11.41 SKK57 pKa = 10.61 GRR59 pKa = 11.84 TSYY62 pKa = 10.52 GSRR65 pKa = 11.84 YY66 pKa = 8.81 RR67 pKa = 11.84 PSYY70 pKa = 9.18 RR71 pKa = 11.84 KK72 pKa = 9.74 KK73 pKa = 9.93 RR74 pKa = 11.84 YY75 pKa = 8.08 SRR77 pKa = 11.84 KK78 pKa = 9.33 NRR80 pKa = 11.84 SSMDD84 pKa = 3.19 GASAVPPPSQSEE96 pKa = 4.32 RR97 pKa = 11.84 PRR99 pKa = 11.84 QPSTTCWQSRR109 pKa = 11.84 WCIRR113 pKa = 11.84 HH114 pKa = 5.29 GVMILIQVLVTWARR128 pKa = 11.84 LSGLLPILSSALTPHH143 pKa = 5.39 MHH145 pKa = 6.94 KK146 pKa = 10.33 SQAIAVRR153 pKa = 11.84 ALLRR157 pKa = 11.84 TKK159 pKa = 10.72
Molecular weight: 17.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.175
IPC2_protein 10.482
IPC_protein 11.506
Toseland 11.564
ProMoST 12.003
Dawson 11.579
Bjellqvist 11.55
Wikipedia 12.032
Rodwell 11.403
Grimsley 11.637
Solomon 12.032
Lehninger 11.93
Nozaki 11.564
DTASelect 11.55
Thurlkill 11.564
EMBOSS 12.047
Sillero 11.564
Patrickios 11.111
IPC_peptide 12.032
IPC2_peptide 11.008
IPC2.peptide.svr19 8.925
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
510
159
351
255.0
29.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.863 ± 2.704
1.961 ± 0.374
3.725 ± 1.646
5.098 ± 1.707
2.941 ± 0.895
7.059 ± 0.743
3.529 ± 0.539
4.706 ± 0.83
4.706 ± 0.161
8.039 ± 0.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.549 ± 0.317
2.941 ± 0.226
7.255 ± 0.179
4.314 ± 0.382
10.0 ± 1.705
8.039 ± 1.744
4.706 ± 0.507
4.902 ± 0.934
2.549 ± 0.352
4.118 ± 0.82
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here