Amino acid dipepetide frequency for Mycobacterium phage RhynO

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.797AlaAla: 10.797 ± 1.318
0.82AlaCys: 0.82 ± 0.286
5.057AlaAsp: 5.057 ± 0.729
7.312AlaGlu: 7.312 ± 0.745
2.733AlaPhe: 2.733 ± 0.432
7.243AlaGly: 7.243 ± 0.793
2.05AlaHis: 2.05 ± 0.349
4.373AlaIle: 4.373 ± 0.515
4.852AlaLys: 4.852 ± 0.812
9.157AlaLeu: 9.157 ± 0.916
2.118AlaMet: 2.118 ± 0.384
2.528AlaAsn: 2.528 ± 0.437
6.423AlaPro: 6.423 ± 0.794
3.895AlaGln: 3.895 ± 0.558
5.808AlaArg: 5.808 ± 0.549
4.51AlaSer: 4.51 ± 0.512
5.398AlaThr: 5.398 ± 0.667
8.2AlaVal: 8.2 ± 0.814
2.665AlaTrp: 2.665 ± 0.475
2.46AlaTyr: 2.46 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.82CysAla: 0.82 ± 0.209
0.0CysCys: 0.0 ± 0.0
0.547CysAsp: 0.547 ± 0.16
0.82CysGlu: 0.82 ± 0.24
0.137CysPhe: 0.137 ± 0.085
0.82CysGly: 0.82 ± 0.27
0.137CysHis: 0.137 ± 0.103
0.273CysIle: 0.273 ± 0.12
0.342CysLys: 0.342 ± 0.143
0.615CysLeu: 0.615 ± 0.24
0.342CysMet: 0.342 ± 0.202
0.273CysAsn: 0.273 ± 0.157
0.547CysPro: 0.547 ± 0.254
0.273CysGln: 0.273 ± 0.138
0.752CysArg: 0.752 ± 0.214
0.273CysSer: 0.273 ± 0.109
0.41CysThr: 0.41 ± 0.166
0.615CysVal: 0.615 ± 0.193
0.205CysTrp: 0.205 ± 0.116
0.547CysTyr: 0.547 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
6.492AspAla: 6.492 ± 0.846
0.547AspCys: 0.547 ± 0.18
2.665AspAsp: 2.665 ± 0.45
4.647AspGlu: 4.647 ± 0.679
2.392AspPhe: 2.392 ± 0.387
5.808AspGly: 5.808 ± 0.611
1.572AspHis: 1.572 ± 0.344
2.392AspIle: 2.392 ± 0.41
2.255AspLys: 2.255 ± 0.47
4.988AspLeu: 4.988 ± 0.59
1.367AspMet: 1.367 ± 0.339
1.708AspAsn: 1.708 ± 0.33
5.262AspPro: 5.262 ± 0.666
2.392AspGln: 2.392 ± 0.409
4.647AspArg: 4.647 ± 0.626
3.007AspSer: 3.007 ± 0.467
3.69AspThr: 3.69 ± 0.463
3.758AspVal: 3.758 ± 0.498
1.162AspTrp: 1.162 ± 0.313
2.323AspTyr: 2.323 ± 0.327
0.0AspXaa: 0.0 ± 0.0
Glu
6.628GluAla: 6.628 ± 0.884
0.342GluCys: 0.342 ± 0.141
5.398GluAsp: 5.398 ± 0.629
4.715GluGlu: 4.715 ± 0.64
2.187GluPhe: 2.187 ± 0.33
6.013GluGly: 6.013 ± 0.566
1.435GluHis: 1.435 ± 0.382
2.733GluIle: 2.733 ± 0.368
2.05GluLys: 2.05 ± 0.358
6.287GluLeu: 6.287 ± 0.668
1.64GluMet: 1.64 ± 0.313
3.143GluAsn: 3.143 ± 0.468
3.143GluPro: 3.143 ± 0.535
2.802GluGln: 2.802 ± 0.422
4.715GluArg: 4.715 ± 0.52
3.143GluSer: 3.143 ± 0.436
3.827GluThr: 3.827 ± 0.529
4.647GluVal: 4.647 ± 0.528
1.708GluTrp: 1.708 ± 0.295
1.64GluTyr: 1.64 ± 0.3
0.0GluXaa: 0.0 ± 0.0
Phe
3.212PheAla: 3.212 ± 0.542
0.342PheCys: 0.342 ± 0.192
2.733PheAsp: 2.733 ± 0.44
2.392PheGlu: 2.392 ± 0.395
0.41PhePhe: 0.41 ± 0.189
3.075PheGly: 3.075 ± 0.464
0.615PheHis: 0.615 ± 0.242
1.367PheIle: 1.367 ± 0.288
0.888PheLys: 0.888 ± 0.275
3.007PheLeu: 3.007 ± 0.458
0.683PheMet: 0.683 ± 0.222
1.23PheAsn: 1.23 ± 0.283
1.64PhePro: 1.64 ± 0.349
0.752PheGln: 0.752 ± 0.217
1.572PheArg: 1.572 ± 0.349
1.708PheSer: 1.708 ± 0.283
1.913PheThr: 1.913 ± 0.299
2.802PheVal: 2.802 ± 0.434
0.478PheTrp: 0.478 ± 0.213
0.342PheTyr: 0.342 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
5.672GlyAla: 5.672 ± 0.854
0.752GlyCys: 0.752 ± 0.208
6.218GlyAsp: 6.218 ± 1.035
5.398GlyGlu: 5.398 ± 0.599
3.348GlyPhe: 3.348 ± 0.698
8.063GlyGly: 8.063 ± 1.121
2.187GlyHis: 2.187 ± 0.37
4.51GlyIle: 4.51 ± 0.538
4.647GlyLys: 4.647 ± 0.506
5.877GlyLeu: 5.877 ± 0.689
2.187GlyMet: 2.187 ± 0.323
3.143GlyAsn: 3.143 ± 0.561
7.175GlyPro: 7.175 ± 2.752
3.143GlyGln: 3.143 ± 0.503
5.33GlyArg: 5.33 ± 0.692
5.125GlySer: 5.125 ± 0.639
5.398GlyThr: 5.398 ± 0.672
6.082GlyVal: 6.082 ± 0.796
1.64GlyTrp: 1.64 ± 0.376
3.553GlyTyr: 3.553 ± 0.572
0.0GlyXaa: 0.0 ± 0.0
His
1.367HisAla: 1.367 ± 0.299
0.068HisCys: 0.068 ± 0.07
1.367HisAsp: 1.367 ± 0.314
1.572HisGlu: 1.572 ± 0.363
0.888HisPhe: 0.888 ± 0.269
2.118HisGly: 2.118 ± 0.559
0.82HisHis: 0.82 ± 0.254
1.162HisIle: 1.162 ± 0.248
0.957HisLys: 0.957 ± 0.262
1.845HisLeu: 1.845 ± 0.357
0.273HisMet: 0.273 ± 0.122
0.41HisAsn: 0.41 ± 0.162
1.435HisPro: 1.435 ± 0.276
0.752HisGln: 0.752 ± 0.258
1.845HisArg: 1.845 ± 0.406
0.82HisSer: 0.82 ± 0.259
0.752HisThr: 0.752 ± 0.252
1.298HisVal: 1.298 ± 0.258
0.82HisTrp: 0.82 ± 0.223
0.888HisTyr: 0.888 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
4.92IleAla: 4.92 ± 0.607
0.478IleCys: 0.478 ± 0.213
3.553IleAsp: 3.553 ± 0.486
3.212IleGlu: 3.212 ± 0.431
1.025IlePhe: 1.025 ± 0.32
3.963IleGly: 3.963 ± 0.475
1.162IleHis: 1.162 ± 0.293
1.64IleIle: 1.64 ± 0.412
1.982IleLys: 1.982 ± 0.351
3.348IleLeu: 3.348 ± 0.542
0.615IleMet: 0.615 ± 0.17
1.777IleAsn: 1.777 ± 0.296
3.143IlePro: 3.143 ± 0.392
1.435IleGln: 1.435 ± 0.28
3.212IleArg: 3.212 ± 0.504
2.255IleSer: 2.255 ± 0.534
2.938IleThr: 2.938 ± 0.422
3.69IleVal: 3.69 ± 0.5
0.615IleTrp: 0.615 ± 0.239
0.888IleTyr: 0.888 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
4.237LysAla: 4.237 ± 0.573
0.273LysCys: 0.273 ± 0.117
2.665LysAsp: 2.665 ± 0.413
2.323LysGlu: 2.323 ± 0.442
1.025LysPhe: 1.025 ± 0.289
4.578LysGly: 4.578 ± 0.97
1.435LysHis: 1.435 ± 0.369
1.982LysIle: 1.982 ± 0.39
2.118LysLys: 2.118 ± 0.409
3.417LysLeu: 3.417 ± 0.44
0.547LysMet: 0.547 ± 0.186
1.162LysAsn: 1.162 ± 0.264
2.665LysPro: 2.665 ± 0.503
1.64LysGln: 1.64 ± 0.372
3.417LysArg: 3.417 ± 0.631
1.708LysSer: 1.708 ± 0.328
2.05LysThr: 2.05 ± 0.432
3.28LysVal: 3.28 ± 0.533
0.957LysTrp: 0.957 ± 0.306
0.82LysTyr: 0.82 ± 0.203
0.0LysXaa: 0.0 ± 0.0
Leu
9.567LeuAla: 9.567 ± 1.025
0.683LeuCys: 0.683 ± 0.245
5.467LeuAsp: 5.467 ± 0.496
5.193LeuGlu: 5.193 ± 0.62
2.187LeuPhe: 2.187 ± 0.328
6.287LeuGly: 6.287 ± 0.729
1.572LeuHis: 1.572 ± 0.342
4.168LeuIle: 4.168 ± 0.541
3.553LeuLys: 3.553 ± 0.611
5.193LeuLeu: 5.193 ± 0.625
2.323LeuMet: 2.323 ± 0.364
2.323LeuAsn: 2.323 ± 0.451
5.467LeuPro: 5.467 ± 0.568
2.187LeuGln: 2.187 ± 0.463
6.287LeuArg: 6.287 ± 0.678
4.715LeuSer: 4.715 ± 0.51
4.578LeuThr: 4.578 ± 0.608
4.783LeuVal: 4.783 ± 0.454
1.367LeuTrp: 1.367 ± 0.314
2.597LeuTyr: 2.597 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.87MetAla: 2.87 ± 0.436
0.0MetCys: 0.0 ± 0.0
1.298MetAsp: 1.298 ± 0.296
1.025MetGlu: 1.025 ± 0.214
0.615MetPhe: 0.615 ± 0.208
1.982MetGly: 1.982 ± 0.37
0.342MetHis: 0.342 ± 0.148
1.23MetIle: 1.23 ± 0.29
1.23MetLys: 1.23 ± 0.303
1.162MetLeu: 1.162 ± 0.292
0.273MetMet: 0.273 ± 0.133
1.025MetAsn: 1.025 ± 0.281
1.435MetPro: 1.435 ± 0.317
0.888MetGln: 0.888 ± 0.21
1.777MetArg: 1.777 ± 0.315
1.913MetSer: 1.913 ± 0.34
1.572MetThr: 1.572 ± 0.317
1.435MetVal: 1.435 ± 0.279
0.273MetTrp: 0.273 ± 0.142
0.342MetTyr: 0.342 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.622AsnAla: 3.622 ± 0.556
0.342AsnCys: 0.342 ± 0.167
1.572AsnAsp: 1.572 ± 0.255
1.708AsnGlu: 1.708 ± 0.38
0.752AsnPhe: 0.752 ± 0.214
3.827AsnGly: 3.827 ± 0.497
0.888AsnHis: 0.888 ± 0.238
1.708AsnIle: 1.708 ± 0.461
1.025AsnLys: 1.025 ± 0.232
2.733AsnLeu: 2.733 ± 0.56
0.615AsnMet: 0.615 ± 0.182
0.41AsnAsn: 0.41 ± 0.162
2.528AsnPro: 2.528 ± 0.447
0.683AsnGln: 0.683 ± 0.183
1.913AsnArg: 1.913 ± 0.436
1.367AsnSer: 1.367 ± 0.383
1.503AsnThr: 1.503 ± 0.269
2.528AsnVal: 2.528 ± 0.312
0.82AsnTrp: 0.82 ± 0.214
1.435AsnTyr: 1.435 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
5.603ProAla: 5.603 ± 0.739
0.342ProCys: 0.342 ± 0.175
4.305ProAsp: 4.305 ± 0.526
4.92ProGlu: 4.92 ± 0.514
2.05ProPhe: 2.05 ± 0.307
5.877ProGly: 5.877 ± 0.803
1.298ProHis: 1.298 ± 0.275
2.46ProIle: 2.46 ± 0.365
2.665ProLys: 2.665 ± 0.728
4.305ProLeu: 4.305 ± 0.593
1.298ProMet: 1.298 ± 0.349
1.913ProAsn: 1.913 ± 0.353
3.417ProPro: 3.417 ± 0.546
2.528ProGln: 2.528 ± 0.851
3.007ProArg: 3.007 ± 0.547
3.143ProSer: 3.143 ± 0.402
4.237ProThr: 4.237 ± 0.639
5.057ProVal: 5.057 ± 0.65
1.572ProTrp: 1.572 ± 0.463
1.982ProTyr: 1.982 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
3.485GlnAla: 3.485 ± 0.507
0.273GlnCys: 0.273 ± 0.135
1.845GlnAsp: 1.845 ± 0.435
1.913GlnGlu: 1.913 ± 0.39
1.23GlnPhe: 1.23 ± 0.292
4.373GlnGly: 4.373 ± 1.371
0.41GlnHis: 0.41 ± 0.144
2.323GlnIle: 2.323 ± 0.367
0.82GlnLys: 0.82 ± 0.165
4.032GlnLeu: 4.032 ± 0.597
0.82GlnMet: 0.82 ± 0.215
1.093GlnAsn: 1.093 ± 0.236
2.255GlnPro: 2.255 ± 0.384
1.23GlnGln: 1.23 ± 0.24
2.597GlnArg: 2.597 ± 0.385
1.23GlnSer: 1.23 ± 0.257
1.913GlnThr: 1.913 ± 0.335
2.733GlnVal: 2.733 ± 0.408
0.683GlnTrp: 0.683 ± 0.206
0.957GlnTyr: 0.957 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
6.833ArgAla: 6.833 ± 0.875
1.025ArgCys: 1.025 ± 0.355
3.758ArgAsp: 3.758 ± 0.532
4.852ArgGlu: 4.852 ± 0.615
2.187ArgPhe: 2.187 ± 0.481
5.33ArgGly: 5.33 ± 0.785
1.093ArgHis: 1.093 ± 0.249
3.007ArgIle: 3.007 ± 0.389
3.827ArgLys: 3.827 ± 0.602
5.74ArgLeu: 5.74 ± 0.653
1.777ArgMet: 1.777 ± 0.339
2.392ArgAsn: 2.392 ± 0.413
2.597ArgPro: 2.597 ± 0.413
2.392ArgGln: 2.392 ± 0.503
5.535ArgArg: 5.535 ± 0.605
3.348ArgSer: 3.348 ± 0.497
3.007ArgThr: 3.007 ± 0.506
4.988ArgVal: 4.988 ± 0.628
1.503ArgTrp: 1.503 ± 0.339
2.665ArgTyr: 2.665 ± 0.484
0.0ArgXaa: 0.0 ± 0.0
Ser
4.373SerAla: 4.373 ± 0.562
0.342SerCys: 0.342 ± 0.156
2.87SerAsp: 2.87 ± 0.493
3.758SerGlu: 3.758 ± 0.482
1.845SerPhe: 1.845 ± 0.27
5.193SerGly: 5.193 ± 0.636
1.025SerHis: 1.025 ± 0.294
2.665SerIle: 2.665 ± 0.371
2.05SerLys: 2.05 ± 0.581
4.305SerLeu: 4.305 ± 0.474
1.162SerMet: 1.162 ± 0.268
1.572SerAsn: 1.572 ± 0.329
2.665SerPro: 2.665 ± 0.32
1.913SerGln: 1.913 ± 0.36
3.827SerArg: 3.827 ± 0.478
2.118SerSer: 2.118 ± 0.364
2.665SerThr: 2.665 ± 0.391
3.622SerVal: 3.622 ± 0.501
0.888SerTrp: 0.888 ± 0.256
0.82SerTyr: 0.82 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
6.013ThrAla: 6.013 ± 0.54
0.683ThrCys: 0.683 ± 0.265
3.007ThrAsp: 3.007 ± 0.481
3.963ThrGlu: 3.963 ± 0.496
2.187ThrPhe: 2.187 ± 0.396
5.262ThrGly: 5.262 ± 0.865
1.025ThrHis: 1.025 ± 0.303
2.255ThrIle: 2.255 ± 0.408
2.118ThrLys: 2.118 ± 0.447
4.92ThrLeu: 4.92 ± 0.681
2.05ThrMet: 2.05 ± 0.398
1.777ThrAsn: 1.777 ± 0.355
3.69ThrPro: 3.69 ± 0.402
2.255ThrGln: 2.255 ± 0.383
3.485ThrArg: 3.485 ± 0.598
2.255ThrSer: 2.255 ± 0.478
2.802ThrThr: 2.802 ± 0.328
3.827ThrVal: 3.827 ± 0.627
0.888ThrTrp: 0.888 ± 0.227
1.64ThrTyr: 1.64 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
7.38ValAla: 7.38 ± 0.639
0.752ValCys: 0.752 ± 0.228
4.783ValAsp: 4.783 ± 0.507
5.125ValGlu: 5.125 ± 0.559
2.392ValPhe: 2.392 ± 0.439
5.74ValGly: 5.74 ± 0.909
1.23ValHis: 1.23 ± 0.275
2.87ValIle: 2.87 ± 0.451
3.485ValLys: 3.485 ± 0.502
6.082ValLeu: 6.082 ± 0.688
1.23ValMet: 1.23 ± 0.366
2.323ValAsn: 2.323 ± 0.382
3.69ValPro: 3.69 ± 0.49
2.665ValGln: 2.665 ± 0.488
4.988ValArg: 4.988 ± 0.516
4.715ValSer: 4.715 ± 0.529
4.647ValThr: 4.647 ± 0.474
5.945ValVal: 5.945 ± 0.468
1.435ValTrp: 1.435 ± 0.342
2.255ValTyr: 2.255 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
1.982TrpAla: 1.982 ± 0.478
0.273TrpCys: 0.273 ± 0.14
1.435TrpAsp: 1.435 ± 0.293
1.503TrpGlu: 1.503 ± 0.356
0.683TrpPhe: 0.683 ± 0.208
1.708TrpGly: 1.708 ± 0.346
0.478TrpHis: 0.478 ± 0.225
1.435TrpIle: 1.435 ± 0.297
0.547TrpLys: 0.547 ± 0.203
1.23TrpLeu: 1.23 ± 0.299
0.547TrpMet: 0.547 ± 0.185
0.615TrpAsn: 0.615 ± 0.213
1.23TrpPro: 1.23 ± 0.29
1.093TrpGln: 1.093 ± 0.265
0.957TrpArg: 0.957 ± 0.23
1.162TrpSer: 1.162 ± 0.305
1.025TrpThr: 1.025 ± 0.277
2.05TrpVal: 2.05 ± 0.394
0.82TrpTrp: 0.82 ± 0.285
0.273TrpTyr: 0.273 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.528TyrAla: 2.528 ± 0.414
0.41TyrCys: 0.41 ± 0.161
2.528TyrAsp: 2.528 ± 0.447
1.845TyrGlu: 1.845 ± 0.43
0.888TyrPhe: 0.888 ± 0.201
2.187TyrGly: 2.187 ± 0.3
0.683TyrHis: 0.683 ± 0.236
1.298TyrIle: 1.298 ± 0.349
0.888TyrLys: 0.888 ± 0.261
2.255TyrLeu: 2.255 ± 0.382
0.752TyrMet: 0.752 ± 0.215
1.162TyrAsn: 1.162 ± 0.198
1.572TyrPro: 1.572 ± 0.28
1.298TyrGln: 1.298 ± 0.231
2.255TyrArg: 2.255 ± 0.452
1.162TyrSer: 1.162 ± 0.316
1.777TyrThr: 1.777 ± 0.308
2.392TyrVal: 2.392 ± 0.401
0.547TyrTrp: 0.547 ± 0.18
1.162TyrTyr: 1.162 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (14635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski