Caldibacillus phage CBP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A290GDV5|A0A290GDV5_9CAUD Uncharacterized protein OS=Caldibacillus phage CBP1 OX=2036768 PE=4 SV=1
MM1 pKa = 7.66AIDD4 pKa = 4.36LEE6 pKa = 4.81HH7 pKa = 6.83PVVTAIRR14 pKa = 11.84RR15 pKa = 11.84YY16 pKa = 9.93GYY18 pKa = 9.01PDD20 pKa = 4.3PEE22 pKa = 4.5PEE24 pKa = 4.06PFGDD28 pKa = 3.86DD29 pKa = 3.43FFGNEE34 pKa = 4.4VYY36 pKa = 10.46PGDD39 pKa = 4.32TILVLDD45 pKa = 5.13DD46 pKa = 4.25EE47 pKa = 5.49FFLKK51 pKa = 10.86DD52 pKa = 4.12EE53 pKa = 4.65LSEE56 pKa = 4.04DD57 pKa = 3.79AIAILEE63 pKa = 4.19HH64 pKa = 6.53FGAVEE69 pKa = 4.06ITAKK73 pKa = 10.59

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A290FZK7|A0A290FZK7_9CAUD Roantirepressor OS=Caldibacillus phage CBP1 OX=2036768 PE=4 SV=1
MM1 pKa = 7.87VIISYY6 pKa = 9.19VNKK9 pKa = 8.55GTEE12 pKa = 3.85VTAIRR17 pKa = 11.84QGIRR21 pKa = 11.84KK22 pKa = 8.96IGKK25 pKa = 7.85ICAKK29 pKa = 9.95IMSWPGGWRR38 pKa = 11.84KK39 pKa = 9.64

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

10175

38

560

154.2

17.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.133 ± 0.476

0.953 ± 0.135

5.808 ± 0.237

9.199 ± 0.271

4.098 ± 0.234

6.3 ± 0.322

1.602 ± 0.161

7.892 ± 0.277

9.16 ± 0.399

8.206 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.168

5.17 ± 0.245

3.656 ± 0.206

3.686 ± 0.247

5.435 ± 0.307

4.727 ± 0.25

4.668 ± 0.263

5.789 ± 0.239

1.258 ± 0.101

3.686 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski