Pseudomonas phage JBD68

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JTB2|A0A1W6JTB2_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD68 OX=1542116 GN=jbd68_52 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.54FMLKK6 pKa = 9.89PDD8 pKa = 3.79LQVGVPGQEE17 pKa = 3.5RR18 pKa = 11.84VSSVSVSGLRR28 pKa = 11.84LTIDD32 pKa = 3.31GEE34 pKa = 4.49EE35 pKa = 4.3YY36 pKa = 10.92DD37 pKa = 4.86FSPLAAGGYY46 pKa = 9.16LPPDD50 pKa = 3.97AYY52 pKa = 10.02ISVTPLQEE60 pKa = 3.86VEE62 pKa = 4.07VRR64 pKa = 11.84GDD66 pKa = 3.52FLFVRR71 pKa = 11.84YY72 pKa = 9.16IHH74 pKa = 6.28QVTADD79 pKa = 3.19ISTAYY84 pKa = 9.53CAEE87 pKa = 4.01VEE89 pKa = 4.49PILVEE94 pKa = 3.54VDD96 pKa = 3.88GPVEE100 pKa = 3.93LPKK103 pKa = 10.87

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JTE9|A0A1W6JTE9_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD68 OX=1542116 GN=jbd68_43 PE=4 SV=1
MM1 pKa = 7.97ISIKK5 pKa = 10.58PEE7 pKa = 3.88GFQQQLADD15 pKa = 3.9LTEE18 pKa = 4.29LEE20 pKa = 4.3QRR22 pKa = 11.84QIPYY26 pKa = 8.86ATATALTRR34 pKa = 11.84TAQGLMDD41 pKa = 4.53RR42 pKa = 11.84LRR44 pKa = 11.84DD45 pKa = 3.56EE46 pKa = 4.08MRR48 pKa = 11.84VVFDD52 pKa = 4.2RR53 pKa = 11.84PTPYY57 pKa = 10.22TLNSLLMVPARR68 pKa = 11.84KK69 pKa = 9.51DD70 pKa = 3.29RR71 pKa = 11.84LEE73 pKa = 3.87ARR75 pKa = 11.84VWFKK79 pKa = 11.63DD80 pKa = 3.61EE81 pKa = 4.8ADD83 pKa = 3.79GAQPASVWIAPEE95 pKa = 4.21VYY97 pKa = 10.21GGPRR101 pKa = 11.84RR102 pKa = 11.84NKK104 pKa = 9.51PAEE107 pKa = 3.87LQLRR111 pKa = 11.84AKK113 pKa = 10.65GILPEE118 pKa = 4.25GKK120 pKa = 9.97YY121 pKa = 9.77VVPGAGADD129 pKa = 3.49LDD131 pKa = 3.93RR132 pKa = 11.84YY133 pKa = 10.08GNIRR137 pKa = 11.84RR138 pKa = 11.84GQVTKK143 pKa = 10.52ALSGIRR149 pKa = 11.84GFTQAGYY156 pKa = 9.74NANATDD162 pKa = 3.7SRR164 pKa = 11.84RR165 pKa = 11.84SRR167 pKa = 11.84AKK169 pKa = 10.36GNARR173 pKa = 11.84RR174 pKa = 11.84YY175 pKa = 10.02FVMTRR180 pKa = 11.84KK181 pKa = 9.3GQPIGIAEE189 pKa = 4.02RR190 pKa = 11.84TGRR193 pKa = 11.84GRR195 pKa = 11.84DD196 pKa = 3.23AVSIIMAFVSRR207 pKa = 11.84PSYY210 pKa = 10.27RR211 pKa = 11.84RR212 pKa = 11.84QLSFFEE218 pKa = 4.53IAQQYY223 pKa = 10.6ADD225 pKa = 3.82EE226 pKa = 4.28NLPRR230 pKa = 11.84EE231 pKa = 4.46FEE233 pKa = 4.03VAMRR237 pKa = 11.84GVAARR242 pKa = 11.84FAARR246 pKa = 11.84RR247 pKa = 3.66

Molecular weight: 27.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12457

40

697

191.6

20.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.415 ± 0.474

0.963 ± 0.13

5.579 ± 0.201

6.454 ± 0.304

2.97 ± 0.176

8.421 ± 0.277

1.541 ± 0.143

4.592 ± 0.164

3.821 ± 0.309

9.176 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.138

3.075 ± 0.187

5.162 ± 0.259

4.231 ± 0.232

7.08 ± 0.356

6.189 ± 0.281

5.555 ± 0.292

7.04 ± 0.28

1.694 ± 0.189

2.432 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski