Enterobacter phage phiT5282H

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Novemvirus; Enterobacter virus phiT5282H

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386K5Y7|A0A386K5Y7_9CAUD Uncharacterized protein OS=Enterobacter phage phiT5282H OX=2340712 GN=OGLPLLMI_00007 PE=4 SV=1
MM1 pKa = 7.65SEE3 pKa = 4.62AKK5 pKa = 10.59LNSNFIAIVAGDD17 pKa = 3.49ITVFNYY23 pKa = 10.51DD24 pKa = 3.13GEE26 pKa = 4.47TRR28 pKa = 11.84EE29 pKa = 4.12YY30 pKa = 10.63LSSSVEE36 pKa = 3.84YY37 pKa = 10.29LAVGVGIPANSCTDD51 pKa = 3.06APGEE55 pKa = 4.24SKK57 pKa = 10.93EE58 pKa = 4.61DD59 pKa = 3.5FTICRR64 pKa = 11.84SADD67 pKa = 3.34FTAWEE72 pKa = 4.32YY73 pKa = 11.09VADD76 pKa = 3.91HH77 pKa = 6.76RR78 pKa = 11.84GEE80 pKa = 4.3TVYY83 pKa = 10.0STEE86 pKa = 3.71TGEE89 pKa = 4.2AVIISAPGDD98 pKa = 3.79YY99 pKa = 10.58PDD101 pKa = 5.14DD102 pKa = 4.09TTTLAPPTPYY112 pKa = 9.57DD113 pKa = 3.19TWNGSEE119 pKa = 3.92WVTDD123 pKa = 3.55TKK125 pKa = 10.97AQHH128 pKa = 6.14AADD131 pKa = 3.84VEE133 pKa = 4.12AAEE136 pKa = 4.11QQKK139 pKa = 10.72AALLAEE145 pKa = 4.36AQTTISLWQTEE156 pKa = 4.4LQLGIISDD164 pKa = 3.91EE165 pKa = 4.51DD166 pKa = 3.77KK167 pKa = 11.32ASLIAWMKK175 pKa = 9.52YY176 pKa = 8.43IKK178 pKa = 10.29AVQAVDD184 pKa = 3.28TSKK187 pKa = 11.26APDD190 pKa = 3.44ITWPDD195 pKa = 3.27KK196 pKa = 11.32PEE198 pKa = 3.63

Molecular weight: 21.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386K513|A0A386K513_9CAUD Uncharacterized protein OS=Enterobacter phage phiT5282H OX=2340712 GN=OGLPLLMI_00026 PE=4 SV=1
MM1 pKa = 7.44SNDD4 pKa = 3.62LFRR7 pKa = 11.84EE8 pKa = 4.16LDD10 pKa = 3.5QVFSDD15 pKa = 3.74ILAGTSQAGRR25 pKa = 11.84IRR27 pKa = 11.84TARR30 pKa = 11.84AVGQALRR37 pKa = 11.84KK38 pKa = 9.43SQQQRR43 pKa = 11.84IKK45 pKa = 10.66AQQNPEE51 pKa = 3.82GSPYY55 pKa = 8.19PARR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84VLRR64 pKa = 11.84SQQGIVFVWQGEE76 pKa = 3.95IRR78 pKa = 11.84RR79 pKa = 11.84LKK81 pKa = 9.23NWHH84 pKa = 6.33GGRR87 pKa = 11.84GKK89 pKa = 10.51YY90 pKa = 10.19GRR92 pKa = 11.84TITGFDD98 pKa = 3.6EE99 pKa = 4.01EE100 pKa = 5.1RR101 pKa = 11.84NDD103 pKa = 2.54IRR105 pKa = 11.84TFYY108 pKa = 10.75RR109 pKa = 11.84SDD111 pKa = 3.02IEE113 pKa = 4.75RR114 pKa = 11.84YY115 pKa = 9.35IEE117 pKa = 3.89INTRR121 pKa = 11.84SVRR124 pKa = 11.84RR125 pKa = 11.84STAKK129 pKa = 9.94KK130 pKa = 9.17VPMFQRR136 pKa = 11.84LRR138 pKa = 11.84SYY140 pKa = 11.2RR141 pKa = 11.84FLKK144 pKa = 9.84MRR146 pKa = 11.84ADD148 pKa = 3.57AGGTSVGYY156 pKa = 10.5DD157 pKa = 3.22GVAARR162 pKa = 11.84IARR165 pKa = 11.84VHH167 pKa = 6.16QYY169 pKa = 8.76GQRR172 pKa = 11.84DD173 pKa = 3.57QVGQGAFAKK182 pKa = 10.46YY183 pKa = 9.27PVRR186 pKa = 11.84EE187 pKa = 4.08LLGFTAGDD195 pKa = 3.02EE196 pKa = 4.27RR197 pKa = 11.84MITEE201 pKa = 3.84QVINSLGSAARR212 pKa = 3.6

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

9718

30

988

249.2

27.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.364 ± 0.658

0.978 ± 0.175

6.195 ± 0.306

6.102 ± 0.292

3.55 ± 0.239

7.471 ± 0.299

1.863 ± 0.195

5.176 ± 0.208

5.454 ± 0.286

9.158 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.171

4.137 ± 0.288

4.044 ± 0.242

4.095 ± 0.287

6.606 ± 0.437

6.565 ± 0.307

6.154 ± 0.325

6.349 ± 0.31

1.441 ± 0.171

2.83 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski