Gammapapillomavirus 14
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2ALR3|A0A2D2ALR3_9PAPI E4 OS=Gammapapillomavirus 14 OX=1513259 GN=E4 PE=4 SV=1
MM1 pKa = 7.15 SLKK4 pKa = 10.03 RR5 pKa = 11.84 KK6 pKa = 9.37 KK7 pKa = 10.01 RR8 pKa = 11.84 ASPQDD13 pKa = 3.25 LYY15 pKa = 11.2 RR16 pKa = 11.84 RR17 pKa = 11.84 CAQGGDD23 pKa = 4.63 CIPDD27 pKa = 3.39 VQNKK31 pKa = 9.52 YY32 pKa = 10.79 EE33 pKa = 4.08 NTTIADD39 pKa = 3.7 WLLKK43 pKa = 10.38 IFGSVIYY50 pKa = 10.31 LGNLGIGTGRR60 pKa = 11.84 GTGGSFGYY68 pKa = 10.34 RR69 pKa = 11.84 PFGSAGTGRR78 pKa = 11.84 PAQEE82 pKa = 4.14 LPIARR87 pKa = 11.84 PNVVIDD93 pKa = 4.91 PITPSSLIPVEE104 pKa = 4.41 PGAPSIVPLVEE115 pKa = 3.8 GTPDD119 pKa = 2.91 IGFAGPDD126 pKa = 3.4 AGPTVAGEE134 pKa = 4.4 DD135 pKa = 3.35 IEE137 pKa = 6.19 LYY139 pKa = 10.41 TLTTPTTDD147 pKa = 2.91 VAGVGGGPSVITTEE161 pKa = 4.11 EE162 pKa = 4.57 LEE164 pKa = 4.16 TAIIDD169 pKa = 3.69 AHH171 pKa = 6.53 PAPATPKK178 pKa = 9.75 QVIYY182 pKa = 10.98 DD183 pKa = 3.69 SVAQVAIEE191 pKa = 3.86 THH193 pKa = 6.12 VNPFYY198 pKa = 11.45 NPDD201 pKa = 3.56 ANNLNIYY208 pKa = 9.84 VDD210 pKa = 3.84 PLITGDD216 pKa = 3.9 TVGGSYY222 pKa = 10.75 FEE224 pKa = 5.91 DD225 pKa = 3.6 IPLEE229 pKa = 4.12 RR230 pKa = 11.84 LDD232 pKa = 3.76 LQSFEE237 pKa = 4.69 VEE239 pKa = 4.27 EE240 pKa = 5.01 PPTEE244 pKa = 4.12 STPTSIGGRR253 pKa = 11.84 LTNRR257 pKa = 11.84 ARR259 pKa = 11.84 DD260 pKa = 3.54 LYY262 pKa = 11.31 SRR264 pKa = 11.84 FIQQVPVNEE273 pKa = 4.45 PDD275 pKa = 4.43 FLVQPSRR282 pKa = 11.84 LVQFEE287 pKa = 4.24 IEE289 pKa = 4.63 NPAFDD294 pKa = 5.29 PDD296 pKa = 3.3 VSLIFEE302 pKa = 4.89 RR303 pKa = 11.84 DD304 pKa = 3.43 LEE306 pKa = 4.33 GLRR309 pKa = 11.84 AAPNEE314 pKa = 3.91 EE315 pKa = 3.78 FADD318 pKa = 3.76 IVRR321 pKa = 11.84 LSQPRR326 pKa = 11.84 VTMTQEE332 pKa = 3.5 GNVRR336 pKa = 11.84 VSRR339 pKa = 11.84 IGTKK343 pKa = 10.24 AAITTRR349 pKa = 11.84 SGLTVGPQVHH359 pKa = 5.99 YY360 pKa = 10.75 FMDD363 pKa = 4.37 LSVIEE368 pKa = 4.75 AVPEE372 pKa = 4.29 TIEE375 pKa = 4.38 LQTLNLPNDD384 pKa = 3.69 SNTIVDD390 pKa = 4.86 DD391 pKa = 4.44 LLAHH395 pKa = 5.71 TTFIDD400 pKa = 3.53 PTNAATLHH408 pKa = 5.82 YY409 pKa = 9.49 EE410 pKa = 3.77 EE411 pKa = 5.71 SDD413 pKa = 3.33 IYY415 pKa = 11.31 DD416 pKa = 4.24 ALPEE420 pKa = 4.49 SFDD423 pKa = 3.38 NTQLVLSTNEE433 pKa = 3.92 EE434 pKa = 4.36 GEE436 pKa = 4.53 SLVFPAFTEE445 pKa = 4.36 SGLKK449 pKa = 9.77 TIFVPGFGSILKK461 pKa = 8.48 TSPTIPPSSVIFSNDD476 pKa = 2.3 TDD478 pKa = 3.7 TFLLHH483 pKa = 6.34 VADD486 pKa = 4.98 INLDD490 pKa = 3.8 YY491 pKa = 10.99 DD492 pKa = 3.94 LHH494 pKa = 6.43 PALLPRR500 pKa = 11.84 KK501 pKa = 9.31 RR502 pKa = 11.84 RR503 pKa = 11.84 RR504 pKa = 11.84 LDD506 pKa = 3.09 AFF508 pKa = 3.85
Molecular weight: 55.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.434
IPC2_protein 4.342
IPC_protein 4.317
Toseland 4.139
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.164
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.228
Nozaki 4.38
DTASelect 4.571
Thurlkill 4.151
EMBOSS 4.177
Sillero 4.431
Patrickios 3.795
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.358
Protein with the highest isoelectric point:
>tr|A0A2D2ALT9|A0A2D2ALT9_9PAPI Protein E7 OS=Gammapapillomavirus 14 OX=1513259 GN=E7 PE=3 SV=1
MM1 pKa = 6.82 QAEE4 pKa = 4.42 TRR6 pKa = 11.84 EE7 pKa = 4.26 SLSARR12 pKa = 11.84 FVAQQDD18 pKa = 3.44 IQLSLIEE25 pKa = 4.76 KK26 pKa = 9.56 DD27 pKa = 3.53 SKK29 pKa = 11.28 NIEE32 pKa = 3.72 DD33 pKa = 5.14 HH34 pKa = 5.21 IAYY37 pKa = 9.04 WEE39 pKa = 4.62 AIRR42 pKa = 11.84 KK43 pKa = 8.73 EE44 pKa = 4.13 NVISYY49 pKa = 6.91 YY50 pKa = 10.66 ARR52 pKa = 11.84 KK53 pKa = 8.91 EE54 pKa = 3.72 HH55 pKa = 6.29 MGRR58 pKa = 11.84 LGLQPLPTTAVSEE71 pKa = 4.35 HH72 pKa = 6.4 RR73 pKa = 11.84 AKK75 pKa = 10.62 EE76 pKa = 4.06 AIKK79 pKa = 8.67 MQIYY83 pKa = 10.01 LRR85 pKa = 11.84 SLARR89 pKa = 11.84 SEE91 pKa = 4.2 YY92 pKa = 10.36 GRR94 pKa = 11.84 EE95 pKa = 3.55 PWTLTEE101 pKa = 4.84 CSAEE105 pKa = 4.22 MFNAPPRR112 pKa = 11.84 DD113 pKa = 3.86 CFKK116 pKa = 10.69 KK117 pKa = 10.12 KK118 pKa = 10.28 PIMVTVWFDD127 pKa = 3.37 NDD129 pKa = 3.41 EE130 pKa = 4.56 KK131 pKa = 11.63 NSFPYY136 pKa = 8.96 TAYY139 pKa = 10.29 EE140 pKa = 3.86 YY141 pKa = 10.15 IYY143 pKa = 10.92 YY144 pKa = 10.29 KK145 pKa = 10.58 DD146 pKa = 5.67 DD147 pKa = 3.54 NDD149 pKa = 2.78 VWYY152 pKa = 10.03 KK153 pKa = 10.02 VQGLVDD159 pKa = 3.53 HH160 pKa = 7.51 DD161 pKa = 3.55 GLYY164 pKa = 10.62 FKK166 pKa = 10.49 EE167 pKa = 4.28 HH168 pKa = 6.38 TGDD171 pKa = 3.12 TVYY174 pKa = 11.18 YY175 pKa = 10.5 QLFQPDD181 pKa = 3.45 ALKK184 pKa = 11.1 YY185 pKa = 8.42 GTTGQWTVKK194 pKa = 10.25 FKK196 pKa = 11.09 NQTVLASVTSSTRR209 pKa = 11.84 SFSKK213 pKa = 10.06 RR214 pKa = 11.84 SEE216 pKa = 4.26 VVSGGTTSHH225 pKa = 6.94 ASTSKK230 pKa = 8.15 EE231 pKa = 4.2 TPRR234 pKa = 11.84 GGPVEE239 pKa = 4.07 TSEE242 pKa = 5.27 DD243 pKa = 3.73 PSSPSSTTPRR253 pKa = 11.84 LRR255 pKa = 11.84 SGRR258 pKa = 11.84 RR259 pKa = 11.84 RR260 pKa = 11.84 RR261 pKa = 11.84 QQGEE265 pKa = 3.98 YY266 pKa = 10.18 SSEE269 pKa = 3.92 SRR271 pKa = 11.84 AKK273 pKa = 10.06 RR274 pKa = 11.84 PRR276 pKa = 11.84 VGSDD280 pKa = 2.95 TAPTPDD286 pKa = 3.05 EE287 pKa = 4.07 VGRR290 pKa = 11.84 RR291 pKa = 11.84 SQSVAAHH298 pKa = 6.06 GLPRR302 pKa = 11.84 LRR304 pKa = 11.84 RR305 pKa = 11.84 LQEE308 pKa = 3.73 EE309 pKa = 4.16 ARR311 pKa = 11.84 DD312 pKa = 3.78 PPIILITGRR321 pKa = 11.84 QNTLKK326 pKa = 10.39 CWRR329 pKa = 11.84 NRR331 pKa = 11.84 CKK333 pKa = 10.65 EE334 pKa = 3.82 NEE336 pKa = 4.27 KK337 pKa = 10.25 ISKK340 pKa = 10.3 LFMCCSSVWKK350 pKa = 9.72 WLGTEE355 pKa = 4.31 DD356 pKa = 4.47 NGLSEE361 pKa = 4.51 GKK363 pKa = 8.97 MLIAFRR369 pKa = 11.84 TIDD372 pKa = 3.44 QRR374 pKa = 11.84 TAFINSVTLPKK385 pKa = 10.46 HH386 pKa = 5.41 CTLSLGKK393 pKa = 10.06 LDD395 pKa = 4.05 SLL397 pKa = 4.89
Molecular weight: 45.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.581
IPC2_protein 8.478
IPC_protein 8.375
Toseland 8.916
ProMoST 8.873
Dawson 9.282
Bjellqvist 9.18
Wikipedia 9.443
Rodwell 9.443
Grimsley 9.297
Solomon 9.355
Lehninger 9.311
Nozaki 9.297
DTASelect 9.077
Thurlkill 9.18
EMBOSS 9.428
Sillero 9.355
Patrickios 4.698
IPC_peptide 9.341
IPC2_peptide 7.922
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2378
94
601
339.7
38.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.056 ± 0.397
2.229 ± 0.73
6.56 ± 0.327
6.686 ± 0.676
4.71 ± 0.661
5.299 ± 0.674
1.766 ± 0.228
5.214 ± 0.672
5.341 ± 0.947
9.378 ± 0.628
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.598 ± 0.277
4.794 ± 0.612
6.476 ± 0.808
3.574 ± 0.418
5.509 ± 0.988
7.317 ± 0.525
6.728 ± 0.686
5.929 ± 0.46
1.262 ± 0.251
3.574 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here